...included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI domains....

...event from the company’s perspective, focus on the message and to keep it short. Soon, discussing with strangers...ResearchGate allow you to send personal updates as messages and keep you up-to-date on your collaborators’...and is much more effective than simply sending a message asking how things are going. Science careers where...

... be cultured and expanded in vitro for several passages to produce an adequate number of starting cells...of virus-free hiPSCs usually require about 5-10 passages. Furthermore, working with Sendai virus requires...reduces biosafety concerns involved in production and usage of Sendai virus. Reprogramming efficiency of PBMCs...

...initial paper, we used MUCICAT to optimize the gene dosage of glucose dehydrogenase and generated a strain...higher cargo copy numbers. (D) The effect of gene dosage on N-acetylglucosamine concentration. Image from...construct a strain library with different gene dosages to facilitate swift optimization of strains. Multiple...

... culture post thaw. In general, the higher the passage number of a culture the lower the transfection ...transduction efficiencies. Cultures should only be passaged 20-30 times before discarding and thawing a fresh...Type Culture Collection and freeze as many early passage vials as possible. One problem that often plagues...

... expression purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid...

...search and navigation, while also updating brand messaging, as well as simplifying the page, and making it...radically redesign our homepage to reflect this message. We concentrated on what our homepage actually ...

...viewed by clicking on the links in the "Agree" and "Disagree" columns found in the Reagent Resources table ...upload supporting documentation that agrees or disagrees with the recommendation. The goal is to easily...

...separate non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged...

...with. My inbox currently stands at about 65,000 messages and dealing with email can be half of my day sometimes...translation of results to companies.” Take home message Starting a lab can be a challenging process. Each...

...can propagate faster than the rate of dilution (passage from the infected to a fresh uninfected host cell...phage progeny is not diluted out but gets manually passaged from an infected host-cell culture to an uninfected...

...like cell phone networks. Protein kinases deliver messages to the next members of their pathways, but where...their signals last all impact transduction of the message. To study signaling pathways, scientists frequently...

...your initial query. This can reduce the number of messages in our thread or remove a good 5 minutes from ...great pick-me-up when things get crazy. Take home message Answering customer service questions is a delicate...

... to distilling a message for the appropriate audience and communicating that message in various ways. ...

...Results of Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling...downstream editing. Overall, the real take-home message thus far, based on survey respondents "Final Verdict...

...resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria, allowing them to...help educate the public about proper antibiotic usage. Although these steps are laudable, they likely ...

...discordance plot (right panel) shows the level of disagreement between the non-edited wild type (control) and...base-by-base, the average amount of signal that disagrees with the reference sequence derived from the control...

...advocate. This is the person whose job it is to disagree with the group. They are the ones who ask for ...While the best devil’s advocate is one who truly disagrees with the group (because they will fight more adamantly...

...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...

...Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications, Jan. 2011, pp. 6–12. Crossref, doi...Histochemistry & Cytochemistry, vol. 62, no. 10, SAGE Publications, July 2014, pp. 693–697. Crossref, ...