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  1. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...valproic acid in 200 mL deionized water Sterilize by passage through a sterile 0.22 µm filter. Prepare 10 mL...rpm. Pro-Tip Do not use cells that are over 30 passages. Section 2: Transfection Check the cell density...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ...your cells. Thaw a new vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media...

  3. General Transfection

    Type
    Protocol
    ...antibiotics to the media. Use cells that are below passage 20 for viral production. The optimal mass DNA:mass...

  4. Lentivirus Production

    Type
    Protocol
    ...pen-strep to the media. Use cells that are below passage 15 for viral production. Procedure Seed 293T packaging...

  5. Colony Formation Titering Assay

    Type
    Protocol
    ...your cells. Thaw a new vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media...

  7. AAV Production in HEK293 Cells

    Type
    Protocol
    ...transfection. Thaw a new vial of cells after 30 passages. Procedure Trypsinize and resuspend the HEK293T...
Showing: 1 - 8 of 8 results