...sell at farmers markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown... we decided to make education and outreach a cornerstone of our business, because we are building this...excited to see what the next few months have in store for us. If you’re thinking about making the switch...

...during storage over prolonged periods of time, especially at room temperature or at 4°C. Storing DNA: temperature...way to store and elute the DNA. References Murakami M (2013) Evaluation of DNA Plasmid Storage Conditions...and it will impact the time you will be able to store your sample without having substantial degradation...things when you elute your DNA: where you plan to store it and for how long. The most common solution is...

...As Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working...proteins. There are still things we haven’t solved that stop it from being used. One thing is that Cas9 is toxic...

...Michael Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer...

... and so the lab organized a sampling trip to Yellowstone National Park (YNP). Extremophiles - phylogenetically... can enable applications where reagents must be stored at elevated temperatures for long time periods ...

...graduate work in Professor Jim Collins' lab (then at Boston University, now at MIT) where he worked on understanding...

...Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including only...contaminating AAV DNA. In addition, we wrote an open-source custom Python script that uses the VGS data to determine...data in minutes and the scripts  can be easily customized to detect other sequences of interest.   While...

...Bacterial Glycerol Stocks for Long-term Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term...Bacterial glycerol stocks are important for long-term storage of plasmids. Although you can store your plasmid...for creating a glycerol stock, including additional tips for long-term storage. Protocols...LB agar plate can be stored at 4°C for a few weeks. However, if you want to store bacteria for a longer...keeping the cells alive. A glycerol stock of bacteria can be stored stably at -80°C for many years. Protocol...unexpectedly at -80°C. Freeze the glycerol stock tube at -80°C. The stock is now stable for years, as long as...concentration of long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol. You ...

...pENTR-Cre Cre with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector...Mammalian Green 8406 p252 pPr-CREM/CMV-STOP-luc CREM and CMV-STOP-luc cassette probasin Mammalian Green...Green 8408 p255 pPr-CREM/Ins/CMV-STOP-luc CREM and CMV-STOP-luc cassette, separated by insulator probasin...Expression: Placing a stop codon with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette...Lentiviral Jacks 11584 Lox-Stop-Lox TOPO Cre activates gene of interest by removing Stop sequences; Puro selection...pCMVe-betaAc-STOP-luc Cre dependent luciferase expression Mammalian Green 32145 pJFRC172-10XUAS-loxP>-dSTOP-loxP...pRRlsinPGK_CREGFP_WPRE Cre-GFP fusion PGK Lentiviral Torok-Storb 99249 pVAX1/mTyr-Cre Cre Tyrosinase Mammalian Blank...

...-A major histocompatibility complex, class I, A FLJ26655, HLAA HLA-B major histocompatibility complex,...major histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major histocompatibility complex...HLA-DOB major histocompatibility complex, class II, DO beta DOB HLA-DPA1 major histocompatibility complex, ...major histocompatibility complex, class II, DQ alpha 2 HLA-DXA HLA-DQB1 major histocompatibility complex...major histocompatibility complex, class II, DR alpha HLA-DRA1 HLA-DRB1 major histocompatibility complex... HLA-G major histocompatibility complex, class I, G MHC-G HLA-H major histocompatibility complex, class...

...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...complete. HRP-conjugated antibodies should not be stored in sodium azide, as it acts as an inhibitor to ...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...

...ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14978 pGL3...Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN hFXN Friedreich ataxia Michael Ristow 14980 pGL3...Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal) FXN hFXN Friedreich ataxia Michael Ristow 14987...ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14989 pBlue-hFX...ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14991 pET28...Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 14994 ...Michael Ristow 15403 pBlue-hFX(I154F)(AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15404...

...chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (...Mycobacterium sp. Neisseria sp. Pseudomonas sp. Ralstonia solanacearum Rickettsia sp. Salmonella sp. Shigella...Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii...protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...

...Strains Why Bacteria? E. coli are commonly used to store and replicate plasmids of all types, but beyond ...bacteria like E. coli and their relatively well understood biology to answer many interesting questions....pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA (gRNA) for bacterial gene disruption... Orange , Red , Far-Red , Near-Infrared , Large Stokes Shift , Photoactivatable , Photoconvertible , and...protein of interest in E. coli , B. subtilis and P. pastoris . All plasmids use the same restriction enzymes... light inducible inverter Red light (650 nm) Christopher Voigt When combined with pCph8 and pPLPCB(S),...s). pJT118 31395 PcpcG2 Green Light (532 nm) Christopher Voigt When combined with pPLPCB(S), encodes a...

...nuclease-deficient Cas9 (dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20...with ampicillin resistance, wherein the N20 can be custom designed to target arbitrary sequences in the genome...controlled sgRNA cassette, wherein the GN19 can be custom designed to target sequences in the genome. The...fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either ...editing bacterial genomes 44251 pgRNA-bacteria A customizable gRNA expression plasmid for use with the bacterial...

... spring of 2011, the groups describe a set of customized plasmids that can be used with the recently developed...plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled...already become the best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General...here . References Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...697-8. Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas...

...control by Addgene before being distributed to customers. Details about our production protocols, titering...vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either performed by...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...The preparations are then aliquoted, frozen, and stored at -80 °C. Titer All titering is performed on lentiviral... lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is performed...

... pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in ...one to efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description... (TAL Orthongal Repressors) that can be used to custom repress gene expression in yeast. TALORs consist... was recently shown that TALE fusions with the histone demethylase LSD1 (Lysine specific demethylase) ...with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation at the target...

...originally discovered as contaminants of adenovirus stocks. One major advantage of using AAV for research ... and learn about our options for hundreds of in-stock preps that are ready to ship right away, as well... lacks adenovirus helper functions required for stock production. Russell 127848 pUCmini-iCAP-PHP.V2 PHP.V2...capsid with the N587 residue replaces with a TAG stop codon Mali 130878 pAAV-DJ-N589X AAV-DJ-N589X Encodes...capsid with the N589 residue replaced with a TAG stop codon Mali 166921 AAV-F in pAR-9 AAV-F AAV rep/cap...

...get started. In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer...Packaged on Request Can't find what you need in our in-stock preps? Our Packaged on Request service offers you... viral vector from Addgene, with details about storage. Biosafety Guide Documentation for your institutional...