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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 249 results for: TIR

Showing: 21 - 40 of 249 results

  1. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: EGFP-expressing...

  2. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...appropriate plasmids, and code the entire page. That would be entirely too much work for one person, it ... expert) the end result is high quality and the entire process is completed more efficiently. Listen to...

  3. Clear and Accessible Writing

    Type
    Blog Post
    ...primarily negative. Stupid, overwhelmed, discouraged, tired…words that indicated there were many barriers between...paper.  Of course, when you think about writing an entire scientific paper, you may feel it’s overwhelming... writing. You can use them to help you write an entire manuscript, or you could use them to write alt ...

  4. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of... to the day before, which got rid of the issue entirely. In many cases, the answer isn't to work harder...ability to focus, especially on days when I was tired, stressed, or rushing. I would spend the time quietly...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...system relies on a transport inhibitor response 1 (TIR1) expressed under a desired promoter with a specific...) sequence attached to your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins...ubiquitin ligase complex. In the presence of auxin, this TIR1 complex binds to the degron sequence ultimately ...

  7. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Most organisms have less than five enzymes in the entire proteome that are biotinylated. In E.coli for example...responsible for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein...recall, BirA biotinylates only one lysine in the entire E.coli proteome. Using this knowledge, scientists...

  8. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...which is ideal for observing and manipulating the entire developmental cycle. A majority of studies involving...most significant advances is the sequencing of the entire axolotl genome, revealing an abundance of genes...year 2000, becoming the first plant to have its entire genome sequenced (Koornneff and Meinke, 2010). ...

  9. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...idea, but they might feel disconnected from the entire scientific process.  I've spent a lot of time debating...debating how to fix this.  As scientists, we work tirelessly in the lab where the groundbreaking research ...training as an academic scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching...

  13. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...constantly influenced by signals it receives from the entire body, making it difficult to probe due to the lack...the fiber horizon? The brain interfaces with the entire body, so the obvious next applications are to expand...

  14. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics... See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5...

  16. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...writing lab reports and empirical essays had also stirred some of my more creative faculties into motion,...neurological health risk. Having played the sport my entire life, I was curious as to what the research had...

  17. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...vector contaminants in your cloning experiments and tired of having to PCR many colonies to find a successful...making a transition into a new job or maybe to an entirely new career path, a career coach might be able ...

  18. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...Addgene Blog Mentoring for Scientists Series. The entire series and additional resources can be downloaded...Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the "Forced Choice...

  19. How Addgene Creates Content

    Type
    Blog Post
    ...truly believes that sharing speeds science, and our entire mission is to enable that process. Our repository... and knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid...
Showing: 21 - 40 of 249 results