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We narrowed to 38 results for: TOP-GFP reporter element

Showing: 21 - 38 of 38 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... three components: a GFP protein that binds to the coding region of a reporter mRNA via a PP7 coat protein...protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter mRNA via an MS2 coat protein...64543or 64542) When the reporter mRNA is first transcribed, it is bound by both the GFP and the RFP proteins...their reporter mRNAs. Using the TRICK plasmids, Halstead et al demonstrated that 91% of reporter mRNAs...customized fluorescent reporters can be challenging enough, but incorporating multiple reporters into a single ...pyralis) reporter plasmids for use in high-throughput screening of pre-mRNA splicing. This reporter system...Compartment mCherry-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP...

  2. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector from the Gage lab encodes GFP and Cre-recombinase.... More detailed descriptions of the lentiviral elements and the 2nd and 3rd Generation Lentiviral Systems...Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning and...and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...Additional Resources on the Addgene blog Viral vector topic page Beginners guide to viral vectors Quick guide...

  3. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following...The vast majority of fly vectors are based on p-element, random or targeted, integration into the fly genome...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g... the protein available – you can always add an epitope tag or a fusion protein. Mammalian vectors One ... allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...e.g. pBAD LIC cloning vector (8A)) and have an epitope tag (6xHis or GST) to affinity purify the expressed...expression pattern of a defined promoter region, ectopically expressing YGOI in a pattern that allows the ...

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...cell body or the axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added...Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Mizushima Autophagy Autophagosome maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation...Clapham Return to Top Cell Physiology Target Description Article PI Autophagy Measure autophagic flux by pH-...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the... the Cas13a molecule brings a much anticipated element of diversity to the CRISPR toolbox and will continue...the Addgene Blog Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which are available..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...and test constructs composed of complex genetic elements. To demonstrate the usefulness of GAMP, the Jacks...allows for easy and rapid targeting of complex elements to specific genomic sites. For instance, the authors... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...transposon: The PB transposon is a mobile genetic element that transposes via a “cut and paste” mechanism...plasmids carry the origin of replication (oriP) element and the cis-acting EBNA-1 from Epstein-Barr virus... iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28...generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming...wide range of cell types and replicates in the cytoplasm independent of the cell cycle. A challenge of ...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... (Dlx5/6) enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating... developed a new AAV approach using regulatory elements that restrict expression to this cell type. To...reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 Listen to the podcast...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...

  10. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn...9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent...Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn... Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent Cre and Flp-dependent...Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...be helpful to the scientific community. Return to top...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Schreiter Calcium Sensor...

  11. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem...ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct...hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to increase transgene protein... translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Reports, 34(13... control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, ...fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Many of the systemic...frequently been used for screening candidate regulatory elements such as enhancers and promoters for cell type-...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer...

  13. Adenovirus Guide

    Type
    Guide
    ...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...in a new window) Adenoviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Common Uses Safety Resources and References Plasmid Elements Glossary Adenoviruses (AdV) are medium-sized, ... cold. The immune system recognizes adenoviral elements rapidly and can mount a strong immune response...plasmid components, see the Adenoviral Plasmid Elements table . Adenoviral Generations First-generation...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...genome. Instead, they remain as extrachromosomal elements within the nucleus, normally as double-stranded...

  14. CRISPR Guide

    Type
    Collection
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...have evolved in phages and other mobile genetic elements to overcome the CRISPR systems that bacteria and...integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated transposases...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...

  15. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead...: 12805549 Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...Pseudotyping Common Uses Safety Resources Plasmid Elements Glossary Gamma-retroviruses are a subtype of retrovirus...plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order to produce gamma-retroviral vectors... sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell ...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...

  16. Lentiviral Vector Guide

    Type
    Guide
    ... type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead...871 PMID: 9306402 Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Pseudotyping Common Uses Safety Resources Plasmid Elements Glossary Lentiviruses are a type of retrovirus...plasmid components, see the Lentiviral Plasmid Elements table . In order to produce lentiviral vectors... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...Tat binds. TAR in cis Trans-activating response element; found in the R region and acts as the binding ...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...

  17. Molecular Biology Reference

    Type
    Guide
    ...plasmid. Below are some common plasmid elements: Plasmid Element Description Origin of Replication ( ori... CRISPR guide . Reporter Plasmids - Used for studying the function of genetic elements. These plasmids...plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of ...Biology Basics Origins of Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids...easily study and manipulate genes and other genetic elements using specifically engineered plasmids, commonly...guide on molecular cloning techniques . Plasmid Elements Plasmids used by scientists today come in many...unique restriction enzyme recognition site. These elements allow for the propagation of the plasmid within...

  18. CRISPR Guide

    Type
    Guide
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...have evolved in phages and other mobile genetic elements to overcome the CRISPR systems that bacteria and...integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated transposases...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...
Showing: 21 - 38 of 38 results