We narrowed to 39 results for: TOP-GFP reporter element

...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...variety of improvements. A synthetic Tet Response Element (TRE) typically features seven copies of tet O ...been designed by combining tet O sequences with elements like the H1 RNA promoter. And, most importantly...center: Tet-Off; right: Tet-On. TRE: Tet response element; tTA: tetracycline-controlled transactivator (fusion...activation domain). For simplicity, other promoter elements and transcription machinery are not shown. Tetracycline...

... 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent 1 Scott Sternson 228902... CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent...PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D...be helpful to the scientific community. Return to top...119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP Cre-dependent 5, 9 Scott Sternson... 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson 119744 AAV...AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson 121538 pAAV SYN1...

...Dre-dependent plasmids. Return to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition...system allows scientists to utilize recombination elements such as Cre to turn off the expression of one ...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and... and Flp-FRT — A Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description Vector... and Flp-FRT — A Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector...

...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...cell body or the axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added...Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling...

...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Eligible for Viral Packaging PI Return to top Other Reporters ONE-GO Biosensors Kit includes BRET-based...Mizushima Autophagy Autophagosome maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation...

... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the... the Cas13a molecule brings a much anticipated element of diversity to the CRISPR toolbox and will continue...the Addgene Blog Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing...

...LgBiT, and the authors used a p53 response-element NanoLuc reporter to show that the mutants abolished transcriptional...apart. While the classic Green Fluorescent Protein (GFP) and other fluorescent proteins revolutionized live-cell...This work highlights HiBiT tagging as a sensitive reporter for tracking proteomic cargo flow via EVs. Find...., & Wood, K. V. (2012). Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone...carcinoma in human tissue and in vitro. Oncology Reports, 50(5). https://doi.org/10.3892/or.2023.8631 Tsien...unique surface epitopes for many proteins, but some proteins do not have such epitopes. These classical... to capture true protein behavior. Traditional epitope knock-ins maintain the native protein context, ...

...tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which are available..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...and test constructs composed of complex genetic elements. To demonstrate the usefulness of GAMP, the Jacks...allows for easy and rapid targeting of complex elements to specific genomic sites. For instance, the authors... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using...

...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...transposon: The PB transposon is a mobile genetic element that transposes via a “cut and paste” mechanism...plasmids carry the origin of replication (oriP) element and the cis-acting EBNA-1 from Epstein-Barr virus... iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28...generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming...wide range of cell types and replicates in the cytoplasm independent of the cell cycle. A challenge of ...

... (Dlx5/6) enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating... developed a new AAV approach using regulatory elements that restrict expression to this cell type. To...reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 Listen to the podcast...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...

...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem...ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct...hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to increase transgene protein... translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ...

...enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Reports, 34(13... control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, ...fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations..., adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Many of the systemic...frequently been used for screening candidate regulatory elements such as enhancers and promoters for cell type-...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....Chuapoco, M. R., Flytzanis, N. C., Goeden, N., Christopher Octeau, J., Roxas, K. M., Chan, K. Y., Scherrer...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...have evolved in phages and other mobile genetic elements to overcome the CRISPR systems that bacteria and...integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated transposases...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...

...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...in a new window) Adenoviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Adenoviral Types Common Uses Safety Resources Plasmid Elements Glossary Credits Adenoviruses (AdV) are medium-sized... cold. The immune system recognizes adenoviral elements rapidly and can mount a strong immune response...plasmid components, see the Adenoviral Plasmid Elements table . Adenoviral Generations First-generation...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...genome. Instead, they remain as extrachromosomal elements within the nucleus, normally as double-stranded...

... type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead...: 12805549 Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...Pseudotyping Common Uses Safety Resources Plasmid Elements Glossary Credits Gamma-retroviruses are a subtype...plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order to produce gamma-retroviral vectors... sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell ...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...

... type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead...871 PMID: 9306402 Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Pseudotyping Common Uses Safety Resources Plasmid Elements Glossary Credits Lentiviruses are a type of retrovirus...plasmid components, see the Lentiviral Plasmid Elements table . In order to produce lentiviral vectors... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...Tat binds. TAR in cis Trans-activating response element; found in the R region and acts as the binding ...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...

...promoters, small RNAs, and other genetic elements. Plasmid Elements Plasmids come in many sizes and vary ...Engineering Kits Reporter Plasmids Used for studying the function of genetic elements. These plasmids ...plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout of the activity of the genetic... Recombinant DNA Working with Plasmids Plasmid Elements Types of Plasmids Molecular Cloning Common E. ...easily study and manipulate genes and other genetic elements using specifically engineered plasmids. These ...create and modify plasmids containing the genetic element that you are interested in. Self-replicate — Once...restriction enzyme recognition site (Figure 2). These elements allow for the propagation of the plasmid within...

...Volker Sieber Plasmids with control elements and fluorescent reporters to be used in combination with the...Voytas 93 additional plasmids including regulatory elements, coding sequences, CRISPR parts GreenGate Cloning...a combinatorial library of constructs expressing GFP at 18 levels for rapid characterization in new organisms...MetClo DNA Assembly Kit Bacterial Expression Christopher A. O'Callaghan Toolkit for assembling large DNA...Expression, Plant Expression, Bacterial expression Christopher Dupont 95 plasmids including backbones, fluorescent...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...have evolved in phages and other mobile genetic elements to overcome the CRISPR systems that bacteria and...integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated transposases...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...