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We narrowed to 105 results for: UBC

Showing: 21 - 40 of 105 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...strength Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough...

  2. MXS Chaining

    Type
    Blog Post
    ...generated with MXS-chaining were used to label subcellular structures in live cells. Four individual expression...detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining gave...Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm/ 456nm histone ...

  3. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ..., it cannot be used for subcellular analysis such as morphology or subcellular localization studies. For...raised in the same host species, of the same Ig subclass, and conjugated to the same fluorochrome as your...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... shown to be useful tools for light-mediated subcellular localization in mammalian cell culture and reversible...were used in a variety of experiments including subcellular visualization of 6 different organelles, a cell...dynamics. These fusion proteins have been used as subcellular markers to visualize the dynamics and interactions...proteins can act as markers for visualization of subcellular structure, for performing live-cell imaging experiments...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR; Long terminal repeats; U3...bigger than ∼3 kb are packaged less efficiently. Subcomponents: U3; Unique 3'; region at the 3' end of viral...

  7. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...be from the same host species, have the same Ig subclass, and be in the same fluorophore (e.g., if you ...staining for anti-mouse Ly6C in APC with the IgG2c subclass, you will use the APC IgG2c isotype control). ...

  8. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4. This can be important...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...

  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... cells (e.g., a neuron subtype) and specific subcellular compartments for measurements. All of the sensors...Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally, ...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...lysosome-associated membrane protein, and following its subcellular localization overtime (Subach et al.) Monitor...particular biological event (trafficking to a subcellular location, start of gene expression, development...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... be included in a single PCR, for example for subcloning genes from different templates. All PCRs are ...can be included at the 5’ end of either primer. Subcloning - To move a gene from one vector to another simply...

  13. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...microtubule-destabilizing drugs are impossible to target to specific subcellular regions, laser ablation can damage other cellular...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared to traditional...proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes for proteomic mapping...

  16. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...
Showing: 21 - 40 of 105 results