We narrowed to 54 results for: aav gfp cre

...Chronos-eGFP]-WPRE EF1a Chronos EGFP Flp dependent 2 Ivo Spiegel 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre ...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Jaws GFP Constitutive 5, 8, rg* Edward Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2...Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 ...PdCO mScarlet Cre dependent 1, 5 Ofer Yizhar 198513 pAAV_hSyn-PdCO-EGFP-WPRE Syn PdCO EGFP Constitutive...soma-targeted) GFP Constitutive 9 Edward Boyden 107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-...Deisseroth 122063 pAAV-EF1α1.1-ChrimsonR-GFP EF1a ChrimsonR GFP Constitutive 2 Edward Boyden 124650 pAAV-CamKIIa-C1V1...Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, rg* Edward Boyden 59171...

... a 'Cre-on' dependent manner. 1 Don Arnold 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent...2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby... 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a. 5, rg* Nathaniel Heintz...Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression...with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev 200778 AAV-∆Hapln1 hSyn-driven...99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...

... Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP Cre-dependent 5, 9 ...mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent...dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity...Scott Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson...Sternson 119744 AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson 121538...52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent 1 Scott Sternson 228902 ...Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics AAV Use our chemogenetics AAV to chemically...

...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Edward Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn... Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Eric Schreiter Calcium...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Eric Schreiter Dopamine...Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1...Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our AAV that express ...Synapsin Other Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent...

...Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible... M. Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Hongkui...expression. See our Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not...Wilson 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps that encode site-specific...

...generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved variant...regulation. To create iGluSnFR, the Looger lab wedged GFP between two halves of a bacterially derived periplasmic...increase in fluorescence intensity from the inserted GFP. After characterizing iGluSnFR in vitro, showing ...Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter, FLEXed for expression in Cre-expressing...and beyond. These new iGluSnFR3 variants come in AAV packaged virus aliquots being deposited at Addgene...hSynap-FLEX: Synapsin-1 promoter, FLEXed for expression in Cre-expressing cells. hSynap-FLP: Synapsin-1 promoter... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An...

...mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP 50457 AAV2 pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium...50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-.... Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics...Testing AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE... 114471 AAV5 pAAV-Ef1a-fDIO mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE...Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h 113049 AAV9 pAAV-hSyn-GRAB_DA1m 123308 AAV9, AAVrg pAAV-hSyn-GRAB_NE1m...

...S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE AAV1, AAV5 Biosensor... AAV1, AAV2, AAV9 Biosensor Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s...: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) AAV-PHPeB Optogenetics Jonathan Ting...: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) AAV-PHPeB Optogenetics Jonathan Ting...pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) AAV-PHPeB Optogenetics Jonathan Ting New viral prep...prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral prep pAAV-Ef1a-fDIO EYFP AAV5. AAV8...serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep ...

... from the default GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with a ...Tissue-specific Cre recombination in this mouse generates cells that randomly express either nuclear GFP, membrane...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low...and activate fluorescence of the downstream OFP, GFP, or RFP. Cells that express the construct but do ...protein genes, Cre recombination is required before fluorescence can occur. Cre recombinase can be used ...developing fish via Cre protein microinjection or crossing of Zebrabow animals to an inducible Cre line. The Schier...OFP (orange) (Livet et al., 2007). Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent...

...Voltron2 voltage sensors AAV vectors for targeting microglia The REVeRT Dual AAV Vector System Neurodegeneration...investigators found that administering these AAVs with a dilute Cre-expressing vector resulted in a sparsely-labeled... incorporated in an AAV vector (Figure 5). Efficiency and specificity of these AAV vectors for microglia...self-complementary AAV (scAAV). However, scAAV vectors have limited size compared to single-strand AAV (ssAAV). ...Figure 5: A) Representative confocal images showing GFP expression from ssAAV5s with hIBA1 or hIBA1a promoters...Quantifications showing high microglia specificity of GFP expression for the indicated ssAAV5s. C) Quantifications...Dual AAV Vector System By Vaibhav Kawde To circumvent the payload limitations of traditional AAVs, the...

...dim GFPs The availability of the three dimensional structure of GFP, its brighter mutant S65T-GFP, and...carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown in eyes and clonogenic...sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...strain in which Cre expression causes the induction of both DsRed fluorescence and GFP shRNA. Mice carrying...and demonstrate that they can successfully create GFP fusion proteins with a variety of genes across the...of Dimitri Deheyn recently identified a family of GFP proteins in the cephalochordate Branchiostoma floridae...Cas9 gene editing. Dr. Masahito Ikawa has created a GFP reporter plasmid for scientists to validate the efficacy...

...Cell type-specific manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-...about Professor Cepko's recent work on developing GFP-activated proteins, read our recent blog post. Check...Multiple Genes to Cells for Gene Therapy 28:40-35:10 : GFP Activatable Tools Being Developed in the Cepko Lab...on the Addgene Blog Read our Recent Blog Post on GFP Activable Plasmid Tools Being Developed in the Cepko... with Michael Koeris Get Cepko Lab Tips on using AAVs in Your Experiments  Resources on the Addgene Website...

...license. Libraries are available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color...our blog post on AAV serotypes for more about the tissue tropism of these and other AAVs. Figure...them in our own AAV production processes. Check out our step-by-step protocols for AAV production, purification... In-Stock AAV inventory or use our Packaged on Request service for thousands of eligible AAV plasmids ...packaging plasmids from Addgene By Meghan Rego Attention AAV users: in an effort to expand your gene delivery ...postsynaptic AMPA receptor and is endocytosed, the Cre recombinase payload activates a floxed reporter gene (genetically...created with BioRender.com. When delivered by AAV to various brain regions in mice, ATLAS mediated ...

...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...Applications Viral Vectors 101: An Introduction to AAV Find AAV Calcium Biosensor Vectors at Addgene ...Calcium is arguably one of the most important metal ions in biology (sodium and potassium are up there... expression, and compatible with constitutive or Cre-dependent expression. These sensors have also been... tissue-specific targeting. By employing various AAV serotypes, researchers can precisely direct the calcium... ready to go. Pro tip: Vectors with ready-to-use AAV preparations are labeled with the green virus particle...3):1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’...

...available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff...SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia SL enzymatic ...selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors...

...restraint include an AAV-based sparse labeling system where a limiting amount of AAV are injected into the...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell... the brain of Cre-expressing mice to trigger recombination and expression of a transgene (Lin et al., ...However, this system requires precise titration of the AAV. So far, tools to overcome this challenge in Drosophila...

...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked...and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The team demonstrated...mCyFP1, the Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring...interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict ...to this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox ...

...tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn ...hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKIIa EGFP Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato...104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738...-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden AAV9-X1.1 ...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...

...Gordon Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain...Gordon Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain...Gordon Fishell 83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain...CAG-NLS-GFP AAV expression of nuclear localized GFP with SV40 NLS Viviana Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2...Yulong Li 115892-AAV8 pAAV-Syn-Archon1-KGC-GFP-ER2 AAV-mediated expression of Archon1-KGC-GFP-ER2 under the... 115893-AAV8 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] AAV-mediated expression of Archon1-KGC-GFP-ER2 under...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...

...target gene expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested ...astrocytes (60%) than AAV9 (Ravindra Kumar et al., 2020). AAV9-X1.1 is evolved from AAV9 and transduces GLUT1...    Figure 1: AAV toolkit for targeted gene delivery to the brain. The AAV (a) capsid, (b) cargo...However, these natural serotypes (AAV9, AAVrh10, and to a certain extent, AAV8) have low transduction efficiency...capsids are derived from AAV9, their efficacy is generally compared to the AAV9 serotype or an engineered...Systemic capsids packaging Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse...precious cargo space in AAV vectors. Dual expression systems, which use two or more AAVs to transduce the same...