...share links to research that isn’t behind paywalls. 5. The #1 (re)tweeted science domain by scientists was...domains more often than other scientists   Figure 5: Portion of Tweets with Scientific Domains, Categorized...Tools & Tips: Online Social Networking for Scientists 5 Reasons to Use Reddit for Science Communication 10...use Twitter professionally to network, learn more about alternative careers for scientists, and share cool....” This paper addresses the following questions about scientists on Twitter: who are they? What do they...

...predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking a double-stranded...end-joining, requires only very small homology regions (5-25 bp) for repair, making it easier to construct targeting...deletions common. In contrast, MMEJ uses regions with 5-25 bp of microhomology flanking a DSB to repair DNA...Briefly, the PITCh vector should be constructed with 5’ and 3’ microhomology to the insertion locus flanking...side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies in the genomic locus. The ...stranded breaks allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette...PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA binding sites...

...guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base pair with a target genomic DNA sequence...construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems can also be found at...

...Species Anti-Integrin alpha 5 beta 1 [IPI-ITGA5/ITGB1.2] Integrin alpha-5,Integrin beta-1 Human, Mouse...Rabbit Anti-Integrin alpha 5 beta 1 [IPI-ITGA5/ITGB1.4] Integrin alpha-5,Integrin beta-1 Human, Mouse...-Integrin alpha V beta 5 [IPI-ITGAV/ITGB5.9] Integrin alpha-V,Integrin beta-5 Human, Mouse IgG1 Rabbit... IgG1 Rabbit Anti-Integrin alpha 5 [IPI-MFR5] Integrin alpha-5 Mouse IgG1 Rabbit Anti-Integrin ... IgG2a Mouse Anti-Integrin alpha 5 [IPI-MFR5] Integrin alpha-5 Mouse IgG2a  Rat   Targeting integrins...Human IgG1 Rabbit Anti-Integrin alpha M [IPI-CBRM1/5] Integrin alpha-M Human IgG1 Rabbit Anti-Integrin...Human IgG2a Rat Anti-Integrin alpha M [IPI-CBRM1/5] Integrin alpha-M Human IgG2a Mouse Anti-Integrin...

...this is a great resource to have in your pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app...saved and cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook...enzyme, or name to discover all the information about that restriction enzyme or share it via email with... this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS... new researchers or students beginning to learn about biochemistry. The least this app will do is allow...

...topoisomerase I as this enzyme recognizes the DNA sequence 5´-(C/T)CCTT-3' and digests double stranded DNA at this...and a tyrosyl residue of topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes ...together the PCR product and TOPO Vector.  3. Incubate 5 Minutes at Room Temperature: You can place your reaction... you should reduce your incubation time on ice to 5 minutes (incubating the full 30 minutes will not significantly...significantly improve the transformation efficiency).  5. Select and Analyze 10 White or Light Blue Colonies...restriction digest, or sequencing. Pro tips Do not add 5’ phosphates to your PCR primers; you need that free...temperature, it is not recommended that you exceed the 5 minute time limit (lower transformation efficiencies...

...there have been varied reports of titer losses from 5% to 50% per freeze thaw cycle (Krajden et al., 1999...µm or 0.22 µm filter) or by a centrifugation step (5 minutes at 300-500 g). After removing packaging cell...medium with half the volume typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture...efficiency by 1.5-fold (Stockschläder et al., 1999). 5. Check your packaging cells for infection If your ...PubMed PMID: 10325307. PubMed Central PMCID: PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant ...grown at 30 °C instead of 37 °C. If you’re unsure about the integrity of your virus, perform a diagnostic...Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus New to Virus? Check ...

... vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde Retrograde AAV To target projection...it will be listed on the Function section pages above. You can also do a search of viral preps and filter...

...within both the 5' and 3' LTRs of retro/lentiviral plasmids. U5; Unique 5'; region at the 5' end of the viral...genomic RNA (but found at both the 5' and 3' ends of the provirus). 5’ LTR Acts as an RNA pol II promoter...' end of viral genomic RNA (but found at both the 5' and 3' ends of the provirus). Contains sequences ... 3’ LTR Terminates transcription started by 5' LTR by the addition of a poly A tract just after ...packaging methods. The three components described above (envelope, packaging, and transfer) are supplied...gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses...

...20R] SUR2B Rat Mouse IgG2a 114481 Anti-QKI-5 [N195A/16R] QKI-5 Human Mouse IgG2a 114482 Anti-Synaptotagmin... IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a 128612 Anti-Kv2.1... IgG2a 177570 Anti-mGluR1/5 (Group I) glutamate receptor [N75/33R] mGluR1/5 (Group I) glutamate receptor...Mouse IgG2a 182119 Anti-mGluR1/5 (Group I) glutamate receptor [N75/3R] mGluR1/5 (Group I) glutamate receptor...Mouse IgG2a 206550 Anti-Synaptotagmin-5 [N274/8R] Synaptotagmin-5 Mouse Mouse IgG2a 206552 Anti-Histone... TRIP8b (exon 1a-5) (Mus musculus) recombinant monoclonal antibody. TRIP8b (exon 1a-5) Mouse Mouse IgG2a...190517 Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse...

...they have a chimeric 5'LTR. Second-generation transfer plasmids have a wildtype 5'LTR, which requires ...have a chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including ...Including a chimeric 5'LTR removes the requirement for the HIV Tat protein, thus decreasing the probability of...3rd generation packaging systems have modified the 5’ LTR of the transfer plasmid to eliminate the need...with a 2nd-generation system because the wildtype 5'LTR promoter requires Tat to function. Third-generation...Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr Library... the proper cell line is used; several common laboratory cell lines including 293 were immortalized with...

...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... by email will supersede the general guarantees above. We will have our expert viral vector production...supersede the general estimated turnaround time above. After starting viral production, if we determine...

...Nature. 1988 Jul 28;334(6180):320-5.) PubMed. 2. A segment of the 5' nontranslated region of encephalomyocarditis...Elements Translation in eukaryotes usually begins at the 5’ cap so that only a single translation event occurs...to spark translation initiation independent of the 5’ cap.  IRES elements are very useful and commonly ...S N P G P * (GSG) residues can be added to the 5' end of the peptide to improve cleavage efficiency...of an IRES fluorescent reporter for easy detection.5  Just released: Addgene's Plasmids 101 eBook And now.../journal.pone.0018556. Epub 2011 Apr 29.) PubMed. 5. Scalable signaling mediated by T cell antigen receptor-CD3...genes above with your gene or genes of interest. Some of the plasmids listed in the table above (and their...

...Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually ...Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The ...

...Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives. Cell 152, 5 P1146-1159. https... demethylases; they are dioxygenases that oxidize 5-methylcytosine to create rare DNA bases that are recognized... due to gRNA design restrictions, anything within 5 base pairs should work well, and up to 10 base pairs... 9, 597. https://doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich L, Li C, et al. (2018) OGT binds ...King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA Demethylation...Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...expression, confounding any potential conclusions about the role of methylation (Hrit et al. 2018, Kaas ...

...iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library...Nov;19(11):1371-1375. doi: 10.1038/s41592-022-01638-5. PubMed (Link opens in a new window) rEcoli XpS and...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV ... of a guide RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...

...Fishell 83896-AAV1 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAV9 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain... 140552-AAV9 pAAV-hsyn-GRAB_5-HT1.0 Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons Yulong Li 140553...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

...modifications and homologous sequences are encoded at the 5’ end. First design the template binding region, with...insertion site. Encode half the new sequence at the 5’ end of each primer, with an overlap for homologous...primer by adding a short sequence complementary to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis...codon and homologous region can be included at the 5’ end of either primer. Subcloning - To move a gene...recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA cloning...our Plasmids 101 blog posts Read our blog post on 5 factors to consider before choosing a cloning method... Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Plasmid cloning is an essential...

... Click on the player below to listen to a quick (~5 min) Hot Plasmids podcast that introduces you to 4...entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of our newsletters...Advice on Picking a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org...this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast...with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, ...each one, plug in some headphones to learn more about the latest and greatest plasmids through this podcast...