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Addgene

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Showing: 21 - 40 of 1078 results

  1. Pipetting Protocol

    Type
    Protocol
    ...consider using a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn more...

  5. AAV ddPCR Titration

    Type
    Protocol
    ...control (NTC) should be close to zero (B08). At Addgene, we consider runs with an NTC > 10 to be invalid...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus Guide Virus Blog Posts...

  7. Distribution to Industry

    Type
    Collection
    ...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...protection for material providers. Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf...A portion of Addgene’s collection is available to qualified for-profit entities. Addgene reserves the ...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...For target validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...

  8. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Request: Scope of Service Addgene's Packaged on Request Service is subject to Addgene’s Terms of Purchase and...with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs QC measures...produced through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL ... to toxicity or other reasons For these cases, Addgene will promptly notify you by email within a few ...not yield the titer guaranteed here or by email, Addgene will contact you to offer some options for moving...this analysis, you will be notified by email. If Addgene determines that our current queue is too high and...not expect to meet the general turnaround time, Addgene will notify you by email before, or within 10 business...

  9. All Antibodies

    Type
    Collection
    ...Browse Addgene's collection of recombinant monoclonal antibodies....application-specific validation and quality control by Addgene as well as by our trusted partner labs. On this...antibodies currently available from our catalog. Addgene values transparency and accessibility in research...reactivity. Reactivity is based on validation data from Addgene or trusted partner labs or is predicted based on... on sequence homology with the antigen. Addgene supplies a list of recommended applications based on our...immunohistochemistry, and immunocytochemistry. Addgene will continue to work with both depositing and ...to produce antibodies are available as part of Addgene’s larger Antibody Plasmid Collection , which also...

  10. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...production at Addgene, ordering information, and more. Additional Viral Vector Resources Addgene's Data Hub...general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from Addgene and scientists across the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation ... Viral Vector Packaging Service Addgene produces high-quality viral vector preps from select...is a common step after requesting plasmids from Addgene, and by providing this service, we can save you... to do when you receive your viral vector from Addgene, with details about storage. Biosafety Guide Documentation... the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular...

  11. Viral Production

    Type
    Collection
    ...Production AAV distributed by Addgene has been produced either in-house by Addgene scientists or through collaboration...Browse AAV preps available from Addgene. After receiving your virus from Addgene, read our detailed recipient...lentiviral preps available from Addgene After receiving your virus from Addgene, read our detailed recipient...Overview of Addgene's viral production methods and quality control for lentivirus and AAV. Viral...are titered and subjected to quality control by Addgene before being distributed to customers. Details ... -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania Vector Core...which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital PCR...

  12. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...Additional Resources Read Addgene's Blog Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids...for a summary of the Ginkgo collection at Addgene. Addgene's COVID-19 and Coronavirus Plasmids & Resources... viral proteins in several expression systems. Addgene has partnered with Ginkgo to make these plasmids...expressed or functionally validated by Ginkgo or Addgene. Plasmids Use the search bar or sort buttons to...to COVID-19 that may be of use to scientists. Addgene's Protocol for RNA Extraction Without A Kit...

  13. Antibody Production

    Type
    Collection
    ...Many antibodies distributed by Addgene have been produced in-house by Addgene scientists. They are generated...or by contacting help@addgene.org . Some of the antibodies distributed by Addgene were developed by the...Overview of Addgene's antibody production methods and quality control processes for purified recombinant...titered and undergo stringent quality control by Addgene or a trusted quality control partner before distribution... preparations developed at IPI may differ from Addgene antibodies in “Production & Usage” and “Delivery...identity. For more details on protocols see the Addgene Protocols page or the Neuromab Protocols (Link ...new window) page. Storage and Shipping Stability Addgene does not recommend repeated freeze thaw of antibodies...

  14. Microbiology Resources

    Type
    Collection
    ...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... Plasmids Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range ...additional microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and distributions...regulations. Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model...genes or applications that are not listed here. Addgene Microbiology Resources Plasmid Kits for Microbiology... depositor protocols and gRNA design software. Addgene yeast CRISPR resources Bacterial CRISPR Resources...

  15. Antibodies

    Type
    Collection
    ...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents... Antibodies Addgene distributes ready-to-use recombinant antibodies...experimental data for recommended applications. Addgene values transparency and accessibility in research...All Antibodies Ready to use antibodies? Browse Addgene’s collection of antibodies developed and validated...Production Interested in our production process? At Addgene, we provide transparency in our production process...antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody...

  16. CRISPR References and Information

    Type
    Collection
    ... Information Addgene Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR ... and news published on the Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled...protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene Church ...Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR...activation, inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software...

  17. CRISPR Plasmids and Resources

    Type
    Collection
    ...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety...zebrafish, C. elegans, parasites, and Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background...

  18. Plant Plasmids and Resources

    Type
    Collection
    ...- deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Addgene's plant resources: Find plasmids for use in plants. Plasmid...Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription...Fluorescent Proteins Other Resources Background Addgene’s repository contains many plasmid tools that were...other species. In addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections... and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids containing promoters...Description Promoter PI Return to top Other Resources Addgene Blog Posts RUBY-Red Siliques CRISPR-mediated Plant...

  19. AAV Packaged on Request

    Type
    Collection
    ...experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and...this service. Select any eligible plasmid in the Addgene repository in your choice of: serotype (AAV1, AAV2...production Density centrifugation purification Addgene’s comprehensive quality control process: Titering...remember to share your protocols and results through Addgene’s Data Hub . By contributing your AAV vector experience...let you know whether the item can be packaged. Addgene does not perform functional or in vivo testing.... we produce high-quality viral vector preps at Addgene, including purification, titration, and quality...research-grade viral vector preps. Data Hub Access Addgene’s Data Hub for protocols and results shared by other...

  20. TALEN Guide

    Type
    Collection
    ... TALENs TALEN Guide Addgene's Guide to TALEN Technologies You may also like....Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter Overview of TALEN Technology...these plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 repeats can...and has already become the best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts...Hospital also recently released a TALEN kit through Addgene. This kit, first described in Nature Biotechnology...at the Broad Institute, will be available from Addgene before the end of the year. Dr. Zhang ‘s lab published...could become a key tool in your plasmid toolbox. Addgene has rapidly become an important resource for this...
Showing: 21 - 40 of 1078 results