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We narrowed to 156 results for: ado

Showing: 21 - 40 of 156 results

  1. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...consistently showing rapid development and quick adoption by the community. Our depositing labs continue... and use new tools. Because there’s such rapid adoption, we thought that requests would be biased towards...tools that fill needs in the community. The rapid adoption of new tools, illustrated in Figure 2, and the...

  2. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...marker of neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison... variable without the behavioral test. Another readout may be necessary to prove inhibition of neuronal...Monitoring behavioral output The appropriate behavioral readout for your optogenetics experiment is contained within...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K., Chen, J...., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...the cellular location of proteins, and can be a readout for protein interaction studies such as immunoprecipitation...and after treatment.  Westerns are also a useful readout for a number of cellular assays. They can determine...following immunoprecipitation and are a common readout for subcellular fractionations, a procedure that...

  5. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...recognized and cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave...

  6. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...is a PhD trained scientist at the University of Colorado, Anschutz Medical Campus.  He studies viruses ...currently a postdoctoral fellow at the University of Colorado Anschutz Medical Campus in Aurora, CO where she.... Erin is also the President of Project Bridge Colorado, an advocacy initiative that seeks to bridge the...

  7. Ras Pathway

    Type
    Collection
    ...Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein 1 SCRIB Scribbled...

  9. Science Communication for Everyone

    Type
    Blog Post
    ...Neurodegenerative and brain disorders are frequently overshadowed by other medical conditions, so these topics... The Addictive Brain, Dr. Sadangi is an eLife ambassador, SfN community leader, and an early research ...

  10. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological Material Transfer...by the National Institutes of Health (NIH) and adopted by the Association of University Technology Managers...

  12. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...Less than 9% of plastics are ever recycled, so adopting a few meaningful changes in the lab could make...to make research more environmentally friendly. Adopt one or all of the recycling practices below. Reuse...

  13. Plan Your Experiment

    Type
    Collection
    ...NHEJ repaired DSBs): Provides a semi-quantitative readout of the percentage of alleles that have been mutated...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...window) Tovote P, Esposito MS, Botta P, Chaudun F, Fadok JP, Markovic M, Wolff SB, Ramakrishnan C, Fenno ...

  16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...sequential excitations wavelengths as determined by Rosado et al: see Table 2 for more details on the effect...variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott...

  17. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...Activate the signaling molecule and perform the assay readout.  Of course, this is much easier said than done...the samples, antibodies, activation, and assay readout used. You’ll note that the secondary antibodies...

  18. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...to take. Decide which technique you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden... has to be optimized according to the strategy adopted, and to the specific reaction you are running. ...

  19. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ..., calmodulin or troponin C). Their fluorescent readouts are used to monitor calcium concentrations and...sensor option if you have the setup necessary for readout.   We’ve got a live one (calcium sensors...
Showing: 21 - 40 of 156 results