We narrowed to 46 results for: cas12

...for in vivo research and potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019...Strecker, Jonathan, et al. "Engineering of CRISPR-Cas12b for human genome editing." Nature communications...PMC6342934. Teng, Fei, et al. "Repurposing CRISPR-Cas12b for mammalian genome engineering." Cell discovery...

...CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates double...ends” rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing than SpCas9 ...Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM which makes it easier...

...: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of ...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene ...

... Type V-K CAST systems use a nuclease-deficient Cas12k (Gelsinger et al., 2024). In either type of CAST...systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K systems; Cascade shown) combines with...

...appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide...

...simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures...

...dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates staggered DNA nicks... Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered...

...Additionally, alternative Cas enzymes exist, such as Cpf1/Cas12a, which recognizes the PAM sequences of TTTV. While... sequence  Additional notes SpCas9 NGG - Cas12a TTTV Great for AT-rich regions Cas9 EQR NGAG...

... It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a may promote...

... liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers yet published...Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat Commun 10: .https...

.... elegans. In 2017, the Korswagen lab developed Cas12a (Cpf1) for editing in C. elegans (Ebbing et al....motif required by the Acidaminococcus sp. BV3L6 Cas12a (AsCpf1) eases this constraint and allows editing...

.... Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, ...

...COVID-19 research, including: CRISPR tools, like Cas12a and Cas13a plasmids, to reliably detect nucleic...

...cancer research. The Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library...

...Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing!  A recent...

...the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from...

...be useful for targeting ncRNAs. For example, the Cas12a variant can cleave single-stranded RNA using gRNAs...

...selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa...

...with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact that it only has...

...CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and...