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  1. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of ...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene ...

  3. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide...

  5. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates staggered DNA nicks... Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered...

  6. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...Additionally, alternative Cas enzymes exist, such as Cpf1/Cas12a, which recognizes the PAM sequences of TTTV. While... sequence  Additional notes SpCas9 NGG - Cas12a TTTV Great for AT-rich regions Cas9 EQR NGAG...

  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ... liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers yet published...Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat Commun 10: .https...

  18. Hot Plasmids - October 2020

    Type
    Blog Post
    ...CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and...
Showing: 21 - 40 of 42 results