...(Cas9, Cas12), ssRNA (Cas13), or ssDNA (Cas14). Efficient Editing and Multiplexing with Cas12a One of ...SHERLOCKv2 to detect multiple targets. For example, Cas12 can be added to detect the presence of infectious...-2 ) and genetic mutations. Similar to Cas9 and Cas12, Cas13 can be converted to an RNA-binding protein...alternatives is Cas12a (Cpf1) . There are many Cas12a orthologs, but the most common is AsCas12a (from Acidaminococcus...The popularity can be attributed to Cas12a’s many benefits. Cas12a exhibits increased efficiency with ...versions of Cas12a recognize different PAM variants, with one of the most popular being EnCas12a, an engineered...of Cas9 exist, along with other Cas enzymes like Cas12a, which are available for specialized applications...

... infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may be...2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific ssDNA degradation to develop the ...DETECTR (Link opens in a new window) Find Doudna Lab Cas12a plasmids Addgene's full CRISPR guide and summary...2020.05.04.20091231 (Link opens in a new window) AapCas12b plasmid from the Abudayyeh-Gootenberg lab. A one-enzyme...

...Doench and Root 2nd 2 40,710 Inzolia Human CRISPR/Cas12a Multiplex Knockout 209551 209552 Knockout Human...Turner N/A 10 13,500 Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library 123361 Knockout Mouse ...

.... Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, ...