We narrowed to 1,698 results for: cas12

1. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

   Plasmid

   #140201

   Purpose

   Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Cloning of crrna an…

   Tags

   dLbCas12a crRNA scaffold

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

   Plasmid

   #149722

   Purpose

   Lentiviral expression of crRNA with GFP and a neomycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFnCas12a_NT

   Plasmid

   #169838

   Purpose

   Expresses FnCas12a and guide RNA in Bacteria

   Depositor

   John van der Oost

   Article

   Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

   Insert

   FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXynA

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-LbCas12a

   Plasmid

   #113431

   Purpose

   Expresses 10xHis-MBP fused to LbCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Has Service

   Cloning Grade DNA

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Topo_SpCas9.tracr_AsCas12a.DR

   Plasmid

   #155050

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pErCas12a EZ Clone

   Plasmid

   #132641

   Purpose

   Expresses ErCas12a and an sgRNA to target a region of interest

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   AAV and CRISPR

   Tags

   3x HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Q926R (Please see depositor comments) and BsaI si…

   Promoter

   CMV Promoter

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS nErCas12an

    Plasmid

    #132642

    Purpose

    Used for in vitro transcription of dual NLS ErCas12a mRNA

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    CRISPR; In vitro transcription

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNHEJ-Cas12a

    Plasmid

    #158713

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK2014-AaCas12b

    Plasmid

    #121949

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    10xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJV53-Cas12a

    Plasmid

    #158706

    Purpose

    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. act5c-LbCas12a

    Plasmid

    #140620

    Purpose

    Ubiquitous expression of LbCas12a in Drosophila

    Depositor

    Michael Boutros

    Article

    Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMBP-AaCas12b

    Plasmid

    #113433

    Purpose

    Expresses Expresses 10xHis-MBP fused to AaCas12b

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AaCas12b
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMV261-Cas12a

    Plasmid

    #158707

    Purpose

    expresses codon-optimized FnCpf1

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ281 (MbCas12a)

    Plasmid

    #138113

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MbCas12a without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    MbCas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AsCas12a-DR-cr in PBCSK-

    Plasmid

    #126640

    Purpose

    Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ284 (Mb2Cas12a)

    Plasmid

    #138116

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    Mb2Cas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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