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We narrowed to 50 results for: cdna expression plasmid

Showing: 21 - 40 of 50 results

  1. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...encoded on separate plasmids and are co-transfected into cells alongside a plasmid encoding the minigenome...four viral genes in the same plasmid (as opposed to four separate plasmids), there are a few reasons arguing...separate (and thus smaller) plasmids. Finally, having four separate plasmids allows researchers to partially... 2 μg minigenome plasmid per well (in a 6 well plate format). However, since plasmid quality and transfection...additional plasmid containing the T7 RNA polymerase gene or using cells that stably express T7 (e.g. BSR...Minigenome System). From a more technical standpoint, a plasmid with all four genes and necessary control elements...relatively large. Cloning the viral genes in this plasmid would be more difficult compared to if each gene...

  2. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression cassettes ...chromosomal integration and gene expression, the system includes a toolkit to express recombinant proteins within...comparisons between CRISPR components expressed from different plasmid backbones. They noted that backbone...or distance in the placement of plasmid elements can impact expression levels and consequently convolute... tools can be used in bacterial and cell-free expression systems, mammalian cells, yeast, flies, and even...from individual transcription units (TUs) used to express your favorite gene-of-interest, all the way to ..., solubility and purification tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... as a donor plasmid containing the transposon, along with a helper plasmid that expresses the transposase...lingering expression of reprogramming factors. Non-replicating Plasmids: Generating iPSCs with plasmid-based...plasmid-based expression requires serial transfection of 1 or 2 plasmids that express the reprogramming factors...reprogramming. Additionally, lingering expression or re-expression of viral transgenes in iPSC-derived cells... generating iPSCs by plasmid transfection where 2 of 11 clones tested had plasmid integration. Another...the dose of plasmid the cells receive over the whole reprogramming period and the plasmid will get diluted...larger plasmids have lower rates of transfection. Minicircles: Minicircles are like mini-plasmids. They...

  4. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...SV40, SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ... mediated genome modification and gene expression. The plasmids associated with this article can be obtained...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase... of the integrases is supplied on its own expression plasmid or in various combinations, while the cognate...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT...

  5. Open Enzyme Collection

    Type
    Collection
    ...Bacterial Expression Vectors Synthetic Biology Plasmids BIOFAB Plasmid Set Drew Endy Lab Plasmids Jennifer...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification... and protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...pOpen-PstIR PstI 165528 pOpen-SfiIR (cDNA) SfiI (used to create cDNA libraries) 165529 pOpen-DpnIR DpnI ...Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology...Jennifer Molloy Lab Plasmids Blog: Golden Gate Cloning (Link opens in a new window) The Open Enzyme Collection...

  6. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ... [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for...H. Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts...four reprogramming factors can be delivered and expressed in multiple somatic cells via various methods....Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...transdifferentiated in vivo to pancreatic β-like-cells with the expression of pancreatic and duodenal homoeobox gene1 (PDX1...transdifferentiation is usually carried out by expressing transcription factors specific to the lineage...Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced...

  7. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...move antibiotic resistance genes between plasmids or from plasmids to the bacteria’s genome. In humans, if...Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase so they ...activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise...codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first reported active DNA...increase in transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve...for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular dystrophy...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...proteins are made by connecting its DNA code to the cDNA encoding for another protein. Effectively, it equips... "Live cell imaging of phosphoinositides with expressed inositide binding protein domains." Methods 46.3...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

  9. Plasmid Collections

    Type
    Collection
    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 162,525 plasmids . We've...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion... Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids from a depositor...

  10. Cancer Research Plasmids and Resources

    Type
    Collection
    ...component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR ...knockdown, and overexpression constructs. Each collection page listed below organizes the plasmids and resources...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Special Collections Cancer Research Cancer Research Plasmids and Resources Addgene has a large variety of resources...Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn ,...vectors from the Sabatini & Wood Labs containing cDNAs for studying oncogenic signaling pathways. ClonTracer...™) to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53...

  11. Guide to Using Pooled Libraries

    Type
    Guide
    ... In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains...different plasmids. Plasmids within a given library have the same backbone, but they express or target... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To: Pooled Library... others, notably pooled lentiviral plasmid libraries, the plasmids must first be used to make virus. This...infected cell receives only one plasmid. To make sure that every plasmid is adequately represented in the... given plasmid, think of each cell carrying it as being a biological replicate for that plasmid. Library...collected, their plasmids are PCR-amplified and sequenced using NGS to identify their cDNA or target gene...

  12. CRISPR Plasmids - Tagging

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    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms..., which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG...well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid. Note that the principle...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...

  13. Neurodegeneration Research Collection

    Type
    Collection
    ...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more....bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...lines expressing it. The Church lab fixed this issue by creating a new dCas9-VPR expression plasmid that...transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila and yeast...Cas9 nickase, and Cas9 activator expressing plasmids that can co-express 1-8 gRNAs. Ordon et al., 2016 tested...p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression 74334 pCR8-Magneto2.0...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ...

  15. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...as close as possible to the basal expression level, induce expression at an opportune time or go all out... following the expression pattern of a defined promoter region, ectopically expressing YGOI in a pattern...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the CMV ...for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression vectors... in molecular biology to isolate, multiply, or express the insert they carry in the target cell. These...most important thing you need to know is your expression system or environment. The host organism will...

  16. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ... of all gamma-retroviral plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or ...capacity Packaging plasmid — contains packaging genes gag and pol Envelope plasmid — contains packaging...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...stably express gag-pol and/or env , eliminating the need to deliver these genes on separate plasmids ( in...different plasmids containing the transfer, envelope, or packaging components. The transfer plasmid encodes...

  17. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...deposited mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core...demethylation in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation ... are an additional layer of control over gene expression that go beyond genomic sequence. Dysregulation...modifications are correlated with alterations in gene expression, but causality and mechanisms remain unclear....that help package DNA, were associated with gene expression or silencing. To alter DNA modifications, researchers...well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds H3K27ac marks...Tet1CD, is available from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure...

  18. Lentiviral Vector Guide

    Type
    Guide
    ...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains...Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains...systems Packaging Plasmid One plasmid encoding gag , pol , tat , and rev Two plasmids, one encoding gag...Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ...separated into distinct plasmids for safety. For a summary of all lentiviral plasmid components, see the ...four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest ...rev ; separated into two plasmids in third generation systems Envelope plasmid — contains packaging gene...

  19. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post
    ...BioRender.com.   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important...comparison of an shRNA plasmid sequence and an miRNA plasmid sequence. (A) Plasmid sequence from pLVUHshp53... transiently expressed using either AAV backbones or species-specific expression plasmids. You can also...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...more flexibility in expression levels and tissue specificity. Using your shRNA plasmids One of the most common...with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let's Go! Browse All Plasmids 101 Posts Additional...

  20. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ... pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein ...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G ...control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster, as ...
Showing: 21 - 40 of 50 results