We narrowed to 495 results for: control

...pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng....LacZ.bGH Control James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. ...eGFP.WPRE.rBG Control James M. Wilson AV-1-PV1963 105542-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG Control James M....pENN.AAV.CMVs.TurboRFP.WPRE.RBG Control James M. Wilson AV-1-PV2642 105552-AAV1 pENN.AAV.hSyn.TurboRFP.WPRE.RBG Control James M...eYFP.WPRE.hGH Control Karl Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James....SV40 Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren ....EGFP.WPRE.bGH Control James M. Wilson AV-2-27056 27056-AAV2 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth...

...AAV PHP.eB Controls Wilson New viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New...pAAV-CaMKIIa-EGFP AAV PHP.eB Controls Roth New serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype...  AAV PHP.eB Controls Gradinaru New serotype pAAV-CaMKIIa-mCherry  AAV PHP.eB Controls Deisseroth New...New serotype pAAV-Ef1a-mCherry  AAV PHP.eB Controls Deisseroth New serotype Looking for more viral...

... rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a biological...Listen to this episode of Hot Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed ...protein domains, they have developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et...opens up the potential for spatial and temporal control in many genetic targeting and editing experiments...photostable, and is compatible with optogenetic control. The researchers also showed that Archon1 is functional... both GOIs, expression levels are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning...photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which change in protein...

...SARS-COV-1 plasmids. Export control license process    An export control license, once approved, allows...repository, including rabies plasmids, are export-controlled materials in the USA, where Addgene is located...applicable research project when purchasing export-controlled materials. Addgene will then apply for the license...   Due to recent regulation changes, an export control license for rabies materials may be required for...application process.  More information about export control licenses is available through the US Bureau of ...

...delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls in a variety...virus, and quality control data for each of the materials we distribute. Quality control info can be found...The viral preparations undergo rigorous quality control testing at Addgene meaning they come ready made...used to activate expression of a gene under the control of the tet operator. AAV AAVs are the workhorses...are already available allowing you to chemically control neuronal activity while studying your phenotype...of interest. Optogenetic tools for similarly controlling neuronal activity with light will be available...determined by the serotype. You can use these as controls to determine if your AAVs are getting to the right...

...spatial control. Optogenetics uses genetically encoded tools, such as microbial opsins, to control cellular...tools allow scientists to use light to externally control the location, timing, and reversibility of the ...process. Read on to learn about the various light-controlled CRISPR tools available to researchers - some ...published in early 2015 focused on using light to control transcription. Two separate labs, Moritoshi Sato...gene expression while imparting spatiotemporal control, reversibility, and repeatability. The system developed...expression of the human ASCL1 gene could be spatially controlled in a reversible and repeatable manner. With their...researchers also demonstrated the ability to spatially control gene expression. Both of these techniques take ...

...intersectional studies And more! Control AAV We’re always excited to add new control AAVs to our inventory since...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs ...our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium ...scientists, we now offer trial size 20 µL samples of control AAV that scientists can use for serotype testing...used in combination with Cre for intersectional control of specific neuronal populations. We now offer ... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ... and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825  AAVrg (20 µL...

...Include proper controls To ensure accurate data analysis, include a complete set of controls. Ideally, every...will have a negative control that does not express the target and a positive control that does. The negative... further the shift of a cell from the negative control, the higher the level of expression. A routine ...including sample preparation, staining procedures, and controls. To prevent instrument clogs, ensure that samples...negative control is critical for determining the background level of non-specific staining.  If you’re using...antibodies for detection, include an unstained control with cells that went through the staining procedure...procedure minus the antibody. An unstained control allows you to detect any autofluorescence from your target...

.... Find the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers to not only interpret...many of our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for ...mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control offered by the...for their own experiments. To this end, we offer control AAV in smaller aliquots of 20ul for serotype testing... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ... AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO...

...26973 AAVrg pAAV-hSyn-hChR2(H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret...inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics...more efficient tools for light mediated neuronal control and we are expanding our viral service to make ...own experiments. To this end, we offer specific control AAV in smaller aliquots of 20 ul for serotype testing... service has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing...response to action potentials, is now offered under control of the CAG promoter for ubiquitous expression. ... 44362  AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Controls Plasmid Serotype Name 50459 AAV1 pAAV-hSyn-DIO-mCherry...

...described the first light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2),...scientists have developed many optogenetic tools to control cellular activity with exquisite precision using...light. Optogenetics, or light activated tools, can control everything from electrical activity to gene expression...BioRender.com.   Optogenetic tools Types of control We will discuss three types of tools: light-gated...Some tools turn on and off rapidly with light to control precise timing of the perturbation. Other tools...intra-cellular signaling cascades. Optical methods for controlling GPCR activation have been developed to selectively...types of GPCRs with greater spatial and temporal control than is achieved by traditional pharmacologic methods...

..., VVDfast to control recruitment of an “anchor” to microtubules and iLID-SspB to control the recruitment...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...with popular light-sensitive ion channels used to control activities like neuronal signaling — think of a... a few recently-deposited optogenetic tools to control the cytoskeleton and cell membrane dynamics. First...component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, and more, often driven...specific Rho GTPase/GEF pair (Ridley, 2016). Taking control of a RhoGEF is a great way to stimulate and study...

...CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that...76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441...,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled...76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,...,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled...57,050 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. 57,050 ...18,901 human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 (Discontinued) Human...

...We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner...generation sequencing results to perform quality control on deposited plasmids. We’ve also talked about ...interface. We regularly use Snapgene for our quality control process because of its expansive feature library... main ways we use SnapGene for plasmid quality control are: To identify important plasmid features ...Identifying plasmid features While our quality control process for incoming plasmids can vary based on... quality control... plasmid quality control...

...express CAs9 and are included as a control (WT). Vinculin is a loading control. 3. In terms of making the...undigested control. Sometimes the PCR products can look smeared, so having an undigested control can help...those formed from control (GFP gRNA-targeted) BRAF sites (lanes 3, 4) were used as controls. Duplexes were...). Anti-vinculin Western blotting is a loading control. Pro-Tips: 1. It’s all about the MOI. In retrospect...and BRDN0001146266) ( 76479-LV and 76480-LV) or control GFP gRNA (BRDN0000561167) (80034-LV) were individually... gRNA-transduced Cas9-A549 cells are used as a control. DNA was analyzed by PAGE. The basis of the...sequence), and PCR-amplify the BRAF locus from control cells (the PCR product should contain the wild-...

...right negative control is critical to the success of BiFC experiments. The ideal control is a mutated variant... work. You will also need to generate negative control constructs — more on this later. For more on constructing... measure the fluorescence of a single FP. Your control construct may generate a modest level of fluorescence...intensity of your BiFC constructs to your negative control to determine whether your PIPs actually do interact...has been compromised (Kodama and Hu, 2012). Such controls are necessary because split FPs can associate,...fragments themselves cannot be used as negative controls because their expression level, stability, and...structural information, constructing appropriate controls may requires that you empirically test a number...

...markers. Compensation/reference control(s) Compensation or “reference” controls are samples stained with a ...is not the same as your single color control. Reference controls: Beads or cells? Compensation beads are...mathematical algorithm and single stained reference controls to distinguish one fluorophore from another within...autofluorescence (you can see this in your unstained flow controls). Spectral flow cytometers include a tool called... or spectral flow. In spectral flow, reference controls tell the software to look for that exact signature...using conjugated dyes, it is critical that your controls and your sample antibodies are from the same batch...on a spectral cytometer, you prepare reference controls of both beads and cells for a single fluorophore...

...place the most important controls front and center, and make specialized controls available when needed....sequence and display frequently used genes and control elements. Development of this tool required creating... DNA sequence has changed. Identifying plasmid control elements Coding sequence features are relatively...relatively straightforward to define, but for control elements such as promoters and transcription terminators...obvious. We found that the annotated boundaries for control elements in commercial plasmids were inconsistent...how we use SnapGene during the plasmid quality control process Check out full plasmid sequencing at Addgene...

...probability of artifacts. Off-target controls A seldom used control for targeted CRISPR experiments is ... how to predict and minimize them, and ways to control for and quantify them when they can’t be avoided...putative off-targets would be suitable. How to control for off-target events Sometimes there just isn’...applications, this is ok, but it still must be controlled for and acknowledged as a potential limitation...to perform key experiments on is a good way to control for off targets as well as clonal differences. ...your experiment is the only way to effectively control for off-targets and significantly decrease the ...effectively serve as a transfection or transduction control, a different gRNA would have distinct off-target...

...Photoactivatable Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with GFP ...transmitted by intermediate messengers. Optical control of kinase activity can provide greater spatiotemporal...There is no common method for creating optically controlled kinases. The Solution: In Zhou et al, the Lin... the delayed effects because you can precisely control the timing of kinase activity. 2. Studying negative...photoswitchable kinases in vivo not only allows for precise control of a signaling pathway in the context of a whole...addition, it could be possible to use pdDronpa to control other kinases and proteins. Zhou et al suggest ...Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell signaling by single-chain photoswitchable...