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We narrowed to 494 results for: control

Showing: 21 - 40 of 494 results

  1. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls Bryan Roth New...CN3905) AAV PHP.eB Controls Jonathan Ting New viral prep pAAV-CaMKIIa-EGFP AAV1 Controls Bryan Roth New ...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13038 - pAAV-AiE0743m...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) AAV PHP.eB Controls Jonathan Ting New viral prep AiP12408 - pAAV-AiE0600m-minBG-SYFP2...pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13863 - pAAV-AiE0888m_C4...minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) AAV PHP.eB Controls Jonathan Ting New viral prep AiP13905 - pAAV-AiE0441h...New serotype pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype pAAV_hSyn-PdCO-mScarlet-WPRE...

  2. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive...genes to various brain regions. You can partially control which cells are successfully infected by an AAV...serotype, but occasionally you may want to further control where your gene of interest is expressed.  A cell-type...cell-type specific Cre can provide you with that control in a modular way. For example, say you would like...contains a Cre-dependent mCherry cassette under the control of an otherwise ubiquitous promoter. You could ... make an entirely new mouse line with mCherry controlled by a neuron specific promoter or you could breed...pmSyn-EBFP-Cre AAV. Because Cre expression is controlled by the synapsin promoter, it and therefore mCherry...

  3. Antibody Production

    Type
    Collection
    ... undergo stringent quality control by Addgene or a trusted quality control partner before distribution...Addgene's antibody production methods and quality control processes for purified recombinant antibodies. ...production protocols, titering methods, and quality control are described below. Information for a specific...samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes testing, either ...fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are...Protein expression is compared to an untransfected control and the protein size is confirmed. Residual Plasmid...testing for loss of potency against a non-treated control in one of its recommended assays. If an antibody...

  4. New Viral Vectors - March 2024

    Type
    Blog Post
    ...AAV PHP.eB Controls Wilson New viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New...pAAV-CaMKIIa-EGFP AAV PHP.eB Controls Roth New serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype...  AAV PHP.eB Controls Gradinaru New serotype pAAV-CaMKIIa-mCherry  AAV PHP.eB Controls Deisseroth New...New serotype pAAV-Ef1a-mCherry  AAV PHP.eB Controls Deisseroth New serotype Looking for more viral...

  5. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ... rather blunt instrument for controlling CRISPR. To bring greater control to Acr activity as a biological...Listen to this episode of Hot Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed ...protein domains, they have developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et...opens up the potential for spatial and temporal control in many genetic targeting and editing experiments...photostable, and is compatible with optogenetic control. The researchers also showed that Archon1 is functional... both GOIs, expression levels are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning...photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which change in protein...

  6. Viral Production

    Type
    Collection
    ...Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview of...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma The 293T...Addgene's viral production methods and quality control for lentivirus and AAV. Viral...virus preps are titered and subjected to quality control by Addgene before being distributed to customers...production protocols, titering methods, and quality control are described below. AAV Production AAV distributed...probes targeting the ITR elements and an internal control of known titer (protocol modified from Lock et ...performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures high quality viral vectors by optimizing...

  7. Antibody Guide

    Type
    Collection
    ...negative control. The negative control may be a no-antibody control in which a sample is processed without ...standard curve can serve as the positive control); and a negative control, a sample known not to contain your...interest. The negative control should be biological (no protein expressed), which controls for your antibody...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and a negative...addition to the control used in standard IP, ChIP should additionally use a beads-only control which runs ...the machine. Controls for cell sorting methods Flow cytometry requires a negative control, a small subset...basic steps needed for each application, important controls, and any special considerations to think about...

  8. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...SARS-COV-1 plasmids. Export control license process    An export control license, once approved, allows...repository, including rabies plasmids, are export-controlled materials in the USA, where Addgene is located...applicable research project when purchasing export-controlled materials. Addgene will then apply for the license...   Due to recent regulation changes, an export control license for rabies materials may be required for...application process.  More information about export control licenses is available through the US Bureau of ...

  9. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls in a variety...virus, and quality control data for each of the materials we distribute. Quality control info can be found...The viral preparations undergo rigorous quality control testing at Addgene meaning they come ready made...used to activate expression of a gene under the control of the tet operator. AAV AAVs are the workhorses...are already available allowing you to chemically control neuronal activity while studying your phenotype...of interest. Optogenetic tools for similarly controlling neuronal activity with light will be available...determined by the serotype. You can use these as controls to determine if your AAVs are getting to the right...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...and repressible promoters are powerful tools to control gene expression and study biological targets. The...popular choices for rapid, efficient, and reversible control of gene expression in eukaryotes. To avoid secondary... While TetR and tet O could be a basic tool to control gene expression, the system has been engineered... TRE: Tet response element; tTA: tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional...activation domain); rtTA: reverse tetracycline-controlled transactivator (fusion of mutated TetR with VP16...advance was the Tet-Off system. A tetracycline-controlled transactivator (tTA) was created by fusing TetR...presence of tetracycline. A new reverse tetracycline-controlled transactivator (rtTA) was created by fusing rTetR...

  11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...spatial control. Optogenetics uses genetically encoded tools, such as microbial opsins, to control cellular...tools allow scientists to use light to externally control the location, timing, and reversibility of the ...process. Read on to learn about the various light-controlled CRISPR tools available to researchers - some ...published in early 2015 focused on using light to control transcription. Two separate labs, Moritoshi Sato...gene expression while imparting spatiotemporal control, reversibility, and repeatability. The system developed...expression of the human ASCL1 gene could be spatially controlled in a reversible and repeatable manner. With their...researchers also demonstrated the ability to spatially control gene expression. Both of these techniques take ...

  12. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...intersectional studies And more! Control AAV We’re always excited to add new control AAVs to our inventory since...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs ...our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium ...scientists, we now offer trial size 20 µL samples of control AAV that scientists can use for serotype testing...used in combination with Cre for intersectional control of specific neuronal populations. We now offer ... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ... and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825  AAVrg (20 µL...

  13. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...Include proper controls To ensure accurate data analysis, include a complete set of controls. Ideally, every...will have a negative control that does not express the target and a positive control that does. The negative... further the shift of a cell from the negative control, the higher the level of expression. A routine ...including sample preparation, staining procedures, and controls. To prevent instrument clogs, ensure that samples...negative control is critical for determining the background level of non-specific staining.  If you’re using...antibodies for detection, include an unstained control with cells that went through the staining procedure...procedure minus the antibody. An unstained control allows you to detect any autofluorescence from your target...

  14. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    .... Find the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers to not only interpret...many of our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for ...mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control offered by the...for their own experiments. To this end, we offer control AAV in smaller aliquots of 20ul for serotype testing... Addgene in the next 6 months, pending quality control. For an estimate on availability date or to be ... AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO...

  15. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...26973 AAVrg pAAV-hSyn-hChR2(H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret...inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics...more efficient tools for light mediated neuronal control and we are expanding our viral service to make ...own experiments. To this end, we offer specific control AAV in smaller aliquots of 20 ul for serotype testing... service has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing...response to action potentials, is now offered under control of the CAG promoter for ubiquitous expression. ... 44362  AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Controls Plasmid Serotype Name 50459 AAV1 pAAV-hSyn-DIO-mCherry...

  16. Optogenetics Plasmid Collection

    Type
    Collection
    ...Optogenetics plasmids encode proteins that are controlled by light. Many of these plasmids encode for microbial...the cell. When using these opsins as a tool to control neuronal activity, scientists generally attach ...localization. Many other types of proteins can also be controlled by light, generally by utilizing a light sensitive...fusions can then be used as an optical switch to control protein interactions, dimerization, localization...the table below for plasmids that use light to control a protein or cellular activity. For example, search...

  17. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...non-standard), expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru...(non-standard), expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru...non-standard), expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...plasmid (non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...plasmid (non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...non-standard), expressing Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...non-standard), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Viviana Gradinaru...

  18. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...described the first light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2),...scientists have developed many optogenetic tools to control cellular activity with exquisite precision using...light. Optogenetics, or light activated tools, can control everything from electrical activity to gene expression...BioRender.com.   Optogenetic tools Types of control We will discuss three types of tools: light-gated...Some tools turn on and off rapidly with light to control precise timing of the perturbation. Other tools...intra-cellular signaling cascades. Optical methods for controlling GPCR activation have been developed to selectively...types of GPCRs with greater spatial and temporal control than is achieved by traditional pharmacologic methods...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ..., VVDfast to control recruitment of an “anchor” to microtubules and iLID-SspB to control the recruitment...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...with popular light-sensitive ion channels used to control activities like neuronal signaling — think of a... a few recently-deposited optogenetic tools to control the cytoskeleton and cell membrane dynamics. First...component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, and more, often driven...specific Rho GTPase/GEF pair (Ridley, 2016). Taking control of a RhoGEF is a great way to stimulate and study...

  20. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner...generation sequencing results to perform quality control on deposited plasmids. We’ve also talked about ...interface. We regularly use Snapgene for our quality control process because of its expansive feature library... main ways we use SnapGene for plasmid quality control are: To identify important plasmid features ...Identifying plasmid features While our quality control process for incoming plasmids can vary based on... quality control... plasmid quality control...
Showing: 21 - 40 of 494 results