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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 335 results for: dre

Showing: 21 - 40 of 335 results

  1. Worm Expression Resources

    Type
    Collection
    ...experiments. The C. elegans genome is well studied and hundreds of reporters have been developed and characterized... General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research...fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without PATC, which...

  2. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 206555 Anti-Alpha-2C adrenergic receptor [N330A/80R] Alpha-2C adrenergic receptor Human Mouse IgG2a... Mouse IgG2a 220412 Beta-2 adrenergic receptor [N430/30R] Beta-2 adrenergic receptor Mouse Mouse IgG2a...scFv] N324/2 Rem2 Rat Mouse 219492 Anti-Alpha-2C adrenergic receptor scFv [N330A/80 scFv] N330A/80 Alpha-...Protocols (Link opens in a new window) References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched...

  3. Bacterial Expression Systems

    Type
    Collection
    ...pDawn FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 68940 pRMC2 Pxyl/TetO Anhydrotetracycline... pR_FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 26092 pET15-MHL T7-lacO Lactose/IPTG Escherichia...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PAQR2 ADM adrenomedullin AM ADM2 adrenomedullin 2 AM2, FLJ21135, dJ579N16.4 ADRB1 adrenergic, beta-1-,...-, receptor ADRB1R, B1AR, BETA1AR, RHR ADRB2 adrenergic, beta-2-, receptor, surface ADRB2R, ADRBR, B2AR...MSH-R, SHEP2 MC2R melanocortin 2 receptor (adrenocorticotropic hormone) ACTHR, MGC125798 MC3R melanocortin...

  7. Mammalian RNAi Tools

    Type
    Collection
    ...Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer...

  8. Synthetic Biology - Overview

    Type
    Collection
    ...George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff Hasty...

  9. Antibody Plasmid Collection

    Type
    Collection
    ...recombinant antibodies. Sci Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian expression of mouse...

  11. TALEN Guide

    Type
    Collection
    ... soon with more features, is already receiving hundreds of unique visitors every month. The Voytas/Bogdanove...

  12. Open Enzyme Collection

    Type
    Collection
    ...Vectors Synthetic Biology Plasmids BIOFAB Plasmid Set Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids Blog...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling...

  14. The Pleiades Promoter Project

    Type
    Collection
    ...Chen J, Chen Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK, Glenn R, Hatakka K, Hearty...

  16. Genetic Code Expansion

    Type
    Collection
    ...azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio 127411 pEVOL-pylT-N346A/C348A PylRS M...

  17. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...used in chemogenetics are DREADDs and PSAMs. Using DREADDs DREADDs DREADDS, or designer receptors exclusively... Other DREADDs, such as the KORD receptor, increase this threshold and silence neurons. DREADD-activated...function (Saloman, JL, et al., 2016).   DREADD ligands Several DREADDs, including hM3D(Gq), have been derived...cellular responses. The cellular response set off by a DREADD depends on what G-protein and, consequently, what...what pathway the receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal...popular agonist for muscarinic receptor derived DREADDs has been clozapine-N-oxide (CNO). However, concerns...been raised over the reliability of using CNO as a DREADD agonist due to its ability to be metabolized into...

  18. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...Exclusively Activated by Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of the...inhibition). AAV preps from DREADD plasmids allow you to easily express a DREADD receptor in a specific neuronal...This article was written by Alyssa Cecchetelli and Andrew Hempstead. What do smell, taste and sight have ...Tools for Studying GPCR signaling  So with the hundreds of GPCRs out there, how can scientists decipher...receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. GPCRs in chemogenetics  While there’s still...molecule ligands such as clozapine-N-oxide (CNO). DREADDs are ideal for chemogenetics as they are relatively...no constitutive activity. There are an array of DREADDs that activate different G proteins, work in different...

  19. A Control for All Seasons

    Type
    Blog Post
    ...isotype-matched control antibody. This control addresses situations where a primary antibody not only binds...   Figure 3: A preimmune serum control addresses situations where a primary antibody not only binds...groups, such as aldehydes in fixed samples. To address this, block positively charged groups with generally... species-on-species staining can be partially addressed with stringent staining protocols and the use ...of noise will determine whether it needs to be addressed. In order to correctly interpret your results,...interferes with data interpretation and can be addressed with a label control. In a label control, the ...to more than their target primary antibody. To address this, include a control wherein each primary antibody...

  20. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...because the DREADD receptors need to be located on the plasma membrane to be activated by the DREADD actuator...Receptor Exclusively Activated by Designer Drugs (DREADDs) (Armbruster et al., 2007).  We initially used ...to the hM4Di might improve the transport of this DREADD to the plasma membrane. The haemagglutinin (HA)...interfered with the transport to the membrane of the DREADDs. In studies where the fluorescence is not needed...proteins. For example, we saw that another type of DREADD, hM3Dq, is successfully expressed at the plasma... Smith Y (2019) Ultrastructural localization of DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https...
Showing: 21 - 40 of 335 results