We narrowed to 42 results for: gc

... Tips and FAQ Tips If your target DNA sequence is GC-rich, increase the time of the denaturing. Your 5...helps with denaturation of the DNA, particularly for GC-rich templates. What does each ingredient specifically...

...bp with the template and if the sequence has high GC or AT content, increase the overlapping region of...

...assemble using the Illumina MiSeq platform, such as GC-rich regions and highly repetitive sequences and/...

... termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed by a ...

...interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but a PAM is not always available near...

...Cas9. Third, it can be used to target “difficult” GC-rich regions of the genome, which seem resistant ...

...from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing in AT-rich genomes...

... Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020) Structure-based...

...Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In Situ Capture...

...References 1. Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible...

...plasmids just won’t amplify! Examples of this are the GC-rich ITRs of AAV genomic DNA encoding plasmids, or...

...efficiency such as the length of the RTT overhang, the GC content of the primer binding site, and many more...

...position; (5) avoid overhangs with either 0% or 100% GC.) In fact, the NEB® team’s data showed that high-...

...that of Cas9 (3’-NGG) in both genomic location and GC-content. After sequencing and searching for cellular...

...mismatches more distal to the PAM, and 2) guides with high GC content have greater off-target activity. Short gRNAs...

...after a ‘G’ base because CBE is inefficient in a ‘GC’ sequence context. If this cannot be avoided, select...

...vector that you want to use is very large, has a high GC content, contains a lot of repeats--any of which ...

...nt length, ~60 oC annealing temperature, and ~50% GC content) so that individual parts and/or strings ...

...provided in the original reference library, as well as GC%, reverse compliment sequences, and more. Output_...

... (concentration, given as viral genome copies/mL (GC/mL)), and total injection volume. You’ll often hear...