We narrowed to 41 results for: gc

1. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...bp with the template and if the sequence has high GC or AT content, increase the overlapping region of...

2. A Look at Addgene's QC Process

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   ...assemble using the Illumina MiSeq platform, such as GC-rich regions and highly repetitive sequences and/...

3. Plasmids 101: Terminators and PolyA signals

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   Blog Post

   ... termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed by a ...

4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but a PAM is not always available near...

5. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...Cas9. Third, it can be used to target “difficult” GC-rich regions of the genome, which seem resistant ...

6. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing in AT-rich genomes...

7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ...efficiency such as the length of the RTT overhang, the GC content of the primer binding site, and many more...

8. High Complexity Golden Gate Assembly with NEB’s DAD

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   ...position; (5) avoid overhangs with either 0% or 100% GC.) In fact, the NEB® team’s data showed that high-...

9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   ... Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020) Structure-based...

10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In Situ Capture...

11. MXS Chaining

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    Blog Post

    ...References 1. Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible...

12. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...plasmids just won’t amplify! Examples of this are the GC-rich ITRs of AAV genomic DNA encoding plasmids, or...

13. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...that of Cas9 (3’-NGG) in both genomic location and GC-content. After sequencing and searching for cellular...

14. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post

    ...mismatches more distal to the PAM, and 2) guides with high GC content have greater off-target activity. Short gRNAs...

15. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...after a ‘G’ base because CBE is inefficient in a ‘GC’ sequence context. If this cannot be avoided, select...

16. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    ...vector that you want to use is very large, has a high GC content, contains a lot of repeats--any of which ...

17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post

    ...nt length, ~60 oC annealing temperature, and ~50% GC content) so that individual parts and/or strings ...

18. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ... (concentration, given as viral genome copies/mL (GC/mL)), and total injection volume. You’ll often hear...

19. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ...provided in the original reference library, as well as GC%, reverse compliment sequences, and more. Output_...

20. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    Blog Post

    ... guide RNA (sgRNA) design, and the abnormally low GC content of the Plasmodium falciparum genome. To put...