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We narrowed to 34 results for: gc

Showing: 1 - 20 of 34 results

  1. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ..., the researchers looked at intrinsic properties (GC content or rare codon percentage) and comparative... and optimizing their algorithm, they found that  GC content and rare codon percentage were not good predictors... predictors. There is not much variation in GC content between many organisms and DNA synthesis companies...companies often work within a narrow range for GC content. Rare codons also are not good predictors because...

  2. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...expect titers of 1 × 1013 GC/mL for AAV1, AAV5, AAV8, and AAVrg and 7 × 1012 GC/mL for AAV2. There are no...

  3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...sequence that is over 80% GC. Some IRES sequences also contain regions that are GC rich. Since NGS assembles...particularly difficult to sequence and assemble, including GC rich regions. For example, the CAG promoter, a hybrid...

  4. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...genome copies (GC) per reaction. Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must...concentration can then be used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = ...

  5. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...secondary structure, palindromic nature, and high GC content. This instability can cause partial loss ...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger sequencing...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ... AAV applications.  Tips & FAQs Due to their high GC content and hairpin structures, ITRs are prone to...might fail to correctly read through ITR sequences. GC tails are often located immediately outside of the...

  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... 8.5 x 10e10 GC/mL. The lot of viruses used (100798-AAV, lot #v15287, titer 1.7x10e13 GC/mL and 100799...100799-AAV1, lot # v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...match the size of your template. Amplification of GC-rich plasmids is facilitated by the addition of DMSO...functionally important regions span several kb (or are GC-rich and therefore difficult to sequence), it may...

  15. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...assemble using the Illumina MiSeq platform, such as GC-rich regions and highly repetitive sequences and/...

  19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing in AT-rich genomes...
Showing: 1 - 20 of 34 results