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Addgene

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We narrowed to 41 results for: gc

Showing: 1 - 20 of 41 results

  1. AAV Packaged on Request

    Type
    Collection
    ...Small 10 × 100 µL aliquots 1 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 25 × 100 µL aliquots 2.5 mL Large... a minimum titer of 2 × 10 12 GC/mL and a typical titer of 7 × 10 12 GC/mL. For more details, see our ...

  3. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ..., the researchers looked at intrinsic properties (GC content or rare codon percentage) and comparative... and optimizing their algorithm, they found that  GC content and rare codon percentage were not good predictors... predictors. There is not much variation in GC content between many organisms and DNA synthesis companies...companies often work within a narrow range for GC content. Rare codons also are not good predictors because...

  4. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...expect titers of 1 × 1013 GC/mL for AAV1, AAV5, AAV8, and AAVrg and 7 × 1012 GC/mL for AAV2. There are no...

  5. KLF Research Plasmids

    Type
    Collection
    ...Krüppel-like zinc fingers which bind to CACCC elements or GC-rich regions on DNA to mediate transcriptional activation...

  6. Open Enzyme Collection

    Type
    Collection
    ...165506 pOpen-Eco31I Eco31I 165507 pOpen-NotIR (GC) NotI (GC-rich recognition site) 165508 pOpen-HindIIIR...

  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...genome copies (GC) per reaction. Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must...concentration can then be used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = ...

  9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...secondary structure, palindromic nature, and high GC content. This instability can cause partial loss ...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger sequencing...

  10. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... AAV applications.  Tips & FAQs Due to their high GC content and hairpin structures, ITRs are prone to...might fail to correctly read through ITR sequences. GC tails are often located immediately outside of the...

  12. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...x 10 12 GC/mL to >2 x 10 13 GC/mL and we use an internal reference virus that is 1 x 10 13 GC/mL. We recommend...titer <1 x 10 12 GC/mL, use dilutions 3–6 If sample is expected to have a titer >3 x 10 13 GC/mL, use dilutions...

  13. AAV ddPCR Titration

    Type
    Protocol
    ...based on an AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents the...data Sample Dilution Concentration Titer (GC/mL) Average Titer (GC/mL) AAV Sample 1 6400000 965 3.09E+13 ...sample: $$T = {R*C*D*1000\over V}$$ Where: T = Titer, GC/mL R = Reaction volume (20 µL) C = Copies/µL D = ...

  14. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...match the size of your template. Amplification of GC-rich plasmids is facilitated by the addition of DMSO...functionally important regions span several kb (or are GC-rich and therefore difficult to sequence), it may...

  20. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... Tips and FAQ Tips If your target DNA sequence is GC-rich, increase the time of the denaturing. Your 5...helps with denaturation of the DNA, particularly for GC-rich templates. What does each ingredient specifically...
Showing: 1 - 20 of 41 results