We narrowed to 3 results for: gc

1. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ...x 10 12 GC/mL to >2 x 10 13 GC/mL and we use an internal reference virus that is 1 x 10 13 GC/mL. We recommend...titer <1 x 10 12 GC/mL, use dilutions 3–6 If sample is expected to have a titer >3 x 10 13 GC/mL, use dilutions...

2. AAV ddPCR Titration

   Type

   Protocol

   ...based on an AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies and generally represents the...data Sample Dilution Concentration Titer (GC/mL) Average Titer (GC/mL) AAV Sample 1 6400000 965 3.09E+13 ...sample: $$T = {R*C*D*1000\over V}$$ Where: T = Titer, GC/mL R = Reaction volume (20 µL) C = Copies/µL D = ...

3. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ... Tips and FAQ Tips If your target DNA sequence is GC-rich, increase the time of the denaturing. Your 5...helps with denaturation of the DNA, particularly for GC-rich templates. What does each ingredient specifically...