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We narrowed to 37 results for: halo tag

Showing: 21 - 37 of 37 results

  1. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells...with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human cell...You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization...collection (Link opens in a new window) of fluorescently-tagged human induced pluripotent stem cell (hiPSC) lines...researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell...TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) 114413 MYL7-mEGFP AICSDP-46 mEGFP MLC-2a Sarcomeric...

  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Voltron2 voltage sensors By Brian O’Neill New viral vector preps expressing the Voltron2 voltage indicator...-2 research New viral vector preps of Voltron2 voltage sensors AAV vectors for targeting microglia The...earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an A122D mutation...ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity during...neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements...negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that administering these...changing the dye that becomes conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah...

  4. Control AAV Preps

    Type
    Collection
    ...Fluorophore/Tag Green Red None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity...non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive...dependent 1, 5 Loren Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre dependent 9 Joshua Sanes 45186... 9 Joshua Sanes 209658 pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag CAG None Cre dependent 5 Tim Brown and HHMI-JRC...

  5. Fluorescent Proteins: FRET

    Type
    Collection
    ...organic fluorophores via HaloTag or SNAP-Tag . Read more about self-labeling tags on our blog . Luciferase...QY A R 0 J(λ) Plasmids mTagBFP sfGFP 399 0.64 510 83,000 0.65 4.6 2.6 mTagBFP2-pBAD , sfGFP-pBAD ECFP ...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...expressed from the same cassette is sometimes advantageous, particularly when only a portion of the plasmid... mRNA. However, some bicistronic vectors take advantage of an element called an Internal Ribosome Entry...bicistronic vectors; however, they do have some disadvantages. These elements are quite large (500-600 bp)...vector.3  2A Peptides To overcome some of the disadvantages of the IRES element, scientists have adapted...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...

  7. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep ...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...and subsequent firing of an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride...promoter of the cre recombinase while still taking advantage of the robust expression via the promoter in the...stereotaxic frame with a digital readout. The advantage is you can “zero” your coordinates at bregma which...engineered to express various opsin genes [7-9]. The advantage of using these lines is, if one is crossed with...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...categories of biosensors or browse all the biosensors tagged in our catalog . Use the article links to find ...by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy. 2007;3(5):452-60. Tamotsu Yoshimori...Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy...Hoffman Voltage JEDI-2P voltage indicator for two-photon imaging Sustained deep-tissue voltage recording...Rafael Yuste Voltage Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium...fluorescent voltage sensor. Nat Neurosci. 2014 Apr 22. Michael Lin Voltage Fluorescent voltage reporters...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters...

  11. Optogenetics AAV Preps

    Type
    Collection
    ...PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV ...AAV collection, including channelrhodopsins, halorhodopsins, and more. Viral...

  12. Biosensor AAV Preps

    Type
    Collection
    ...Campbell Calcium Sensor: HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1...Schreiter Calcium Sensor: HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1...jRGECO1 sRGECO Far-Red/NIR Calcium Sensors HaloCaMP1a HaloCaMP1b NIR-GECO Acetylcholine Sensors iAChSnFR ...Sensors GRAB_NE nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ASAP ATP Sensors...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...ST-WPRE Syn Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-...

  13. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...CODA CODA aGTCCGCCTCCACCCGAGGTAGAG zgc:111821 OZ597 and OZ598 CODA CODA tGGCAGCAGCTTTTCGAAGAATAGg zgc:65890...gCGTGACCTCgctgatAAGAAGAGg xylt1 OZ509 and OZ510 OPEN OPEN cACCCTCTACaggacaGTAGCGGGTg zgc:153086 OZ511 and OZ512 OPEN OPEN ...cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt fox1 (a2bp1l) OZ519 and OZ520 OPEN OPEN...aGACTCCGTCccgtcgtGCTGAGGGCc hnf1a OZ521 and OZ522 OPEN OPEN aACCTTCCCCagagaGAGGTAGTGg VDAC2 OZ523 and OZ524 OPEN OPEN gCTCCCAACCactttGCTGGTGTCa...and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc Encephalopsin (Opn3) OZ541 and OZ542 OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt...tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg NRF2b (nfe212b) OZ561 and OZ562 OPEN ...tGTCTCCGGCagaggGAGGAGGAGa esrrga OZ563 and OZ564 CODA CODA tAGCTACCGCTGGCGTCTGAGGAGc HACE1 OZ565 and OZ566 CODA CODA gCTCATCTCCACCGAGCTGGAGAAg...

  14. AAV Viral Preps

    Type
    Collection
    ...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti...

  15. Optogenetics Plasmid Collection

    Type
    Collection
    ... cells. Other microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins...from cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins. These fusions can then ...

  16. Distribution to Industry

    Type
    Collection
    ...Information Featured Collections NEW NanoLuc and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2,...

  17. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...reducing the risk of immune activation, both key advantages of rAAV vectors. AAV Types Serotypes AAV serotypes...immune response, and the low risk of insertional mutagenesis. Several AAV-based gene therapies have been approved...short coding sequences of channelrhodopsins, halorhodopsins, and other optogenetic genes makes them suitable...chromosome, which reduces the risk of insertional mutagenesis. However, random integration events can occur...host organism, leading to disease. Insertional mutagenesis Random insertion of DNA elements into the genome...
Showing: 21 - 37 of 37 results