We narrowed to 315 results for: idua

...cells. Monoclonal antibodies are derived from an individual clone targeting a single antigen. An example ...

...library allows researchers to reconstruct the full individual sequence. Traditional NGS workflows are limited...costly library prep, as each sample requires an individual library. The plexWell™ workflow is a sequential...continuing to process 100s to 1000s of samples individually, plexWell™ reduces every 96 well plate to a ...often arises from the fact that large numbers of individual input DNA samples (e.g. a collection of 96 plasmids... prep methods because the yield or quality of individual libraries can be influenced by the quantity or...usually include a separate normalization step where individual input DNA samples are brought in line with each...normalization, it is also often the case that individual libraries prepared from multiple samples will...

...they cause. The standard format for providing individual plasmids is a bacterial stab (of a laboratory...

...by a specific topic, but can only be ordered individually. CRISPR Read Addgene's CRISPR Guide for a general...

...not require an MTA. Pricing can be found on the individual product pages. Orders of the 20 µg DNA size are...

...nuclease, the components of the array recognize individual nucleotides. For over ten years, zinc finger ...

...assembly. Plasmids Explore the collection and order individual plasmids via the links below: DNA Ligases DNA...

...underrepresented individuals too nicely. When the work of the underrepresented individual is then judged...The National Academies define a postdoc as “An individual who has received a doctoral degree (or equivalent...to pursue his or her chosen career path,” but individual institutions can have many different names for...provided a concrete example of how underrepresented individuals are discluded/not treated equitably even when...it can come as a shock to the underrepresented individual. In an attempt to be nice, the mentor fails to...trainees despite the fact that the underrepresented individual is welcomed into the lab. We need some way of...

...available for request on the Addgene website, both as individual plasmids and as the Fragmid kit. You can also...timeline of the Golden Gate (GG) cloning approach. Individual modules and a sample ligation are depicted. (...Pol 2 protomer, Cas protein, ect...). Whatever individual components you choose will assemble in the expected...lab tested the Fragmid system by sequencing 60 individual assemblies created over five months. When they...one plasmid was the correct sequence for every individual assembly. What destination vectors are available...kit. While you can order each of the plasmids individually, if you’re going to be using more than six or...

...and a variety of other species. In addition to individual plasmids, Addgene carries several useful toolkits...

...sample for further downstream applications. As individual libraries can vary significantly, it’s important... an amplification protocol developed for each individual pooled library; generalized amplification protocols... you need 30 to 200 times as many colonies as individual plasmids in your library. As an example, if you...  Pro tip! Since you probably do not want to individually count 500,000 colonies, you’ll likely rely on... DNA, you will likely need to perform several individual transformations, such as splitting 400 ng of ...of DNA in 100 μL of cells over four individual electroporations. The specifics of this can be based on ...

...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...

...), barcoded for tractability. These ORFs are individually overexpressed and available for lentiviral packaging...identify both up- and down-regulated pathways. Individual cellular fates were then matched with candidate...pooled library. Louvain community clusters of individual cells along with color coded nominated reference...fluorescent controls. Alternatively, you can order individual plasmids from the library to further investigate...to the size of the collection, we’re releasing individual plasmids in batches. If there’s something you...

... together teams of individuals who enjoy and are experts in each of the individual tasks required to develop... but through the coordinated efforts of many individuals. Check out Joanne's Reddit AMA   Benefits of...each component of the project completed by an individual who is a local expert in that task (or has the...Set Concrete Goals with Your Mentor through an Individual Development Plan Resources on the Addgene Website...

...cDNA libraries. However, using CRISPRa to target individual genes can be relatively laborious because multiple...in defined substructures of the CNS as well as individual neuronal populations.  The Yang Lab’s AAV plasmids...straightforward than generating cDNA for each gene isoform individually. To highlight this advantage, they selected ...CRISPRa represents a powerful method to dissect the individual and shared contributions of transcript isoforms...overexpression approaches may still be preferable for individual gene activation in select in vitro and in vivo...

...distinct differences of red-to-green fluorescence in individual mitochondria in the networks marks the differences...in a Golden Gate cloning step to assemble the individual components into a destination vector in a predefined...UTRs, coding sequences, terminators, etc) into individual Level 0 plasmids, or choose from a growing number... assembly system, which reduces the number of individual repeat-variable di-residue (RVD) module plasmids...toolkit for high-resolution fluorescent imaging of individual neurons. The technology generates a unique spectral...TheGreenGate kit available at Addgene includes six individual types of pre-cloned insert modules (plant promoter...

... an individual to another individual perspective? A: CAP-B10 and CAP-B22 were tested individually in 4... 4 marmosets each. Brain potency for each individual is reported in Figure 4B (see left for image and ...

...construct and can be accessed using the links to view individual plasmid webpages. These plasmids are described...

...electroporation. To achieve sparse labeling of individual neurons one could reduce the plasmids amounts... of recombinase restricts their expression to individual cells. With this technique, a Neuroscientist ...inducible Cre recombinase (CreERT2), visualizes individual neurons and their synapses in a conditional knockout...for more. In order to analyze the function of individual proteins in neuronal circuits or synaptic remodeling...

...amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ...