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We narrowed to 318 results for: idua

Showing: 21 - 40 of 318 results

  1. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... together teams of individuals who enjoy and are experts in each of the individual tasks required to develop... but through the coordinated efforts of many individuals. Check out Joanne's Reddit AMA   Benefits of...each component of the project completed by an individual who is a local expert in that task (or has the...Set Concrete Goals with Your Mentor through an Individual Development Plan Resources on the Addgene Website...

  2. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...cDNA libraries. However, using CRISPRa to target individual genes can be relatively laborious because multiple...in defined substructures of the CNS as well as individual neuronal populations.  The Yang Lab’s AAV plasmids...straightforward than generating cDNA for each gene isoform individually. To highlight this advantage, they selected ...CRISPRa represents a powerful method to dissect the individual and shared contributions of transcript isoforms...overexpression approaches may still be preferable for individual gene activation in select in vitro and in vivo...

  3. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...distinct differences of red-to-green fluorescence in individual mitochondria in the networks marks the differences...in a Golden Gate cloning step to assemble the individual components into a destination vector in a predefined...UTRs, coding sequences, terminators, etc) into individual Level 0 plasmids, or choose from a growing number... assembly system, which reduces the number of individual repeat-variable di-residue (RVD) module plasmids...toolkit for high-resolution fluorescent imaging of individual neurons. The technology generates a unique spectral...TheGreenGate kit available at Addgene includes six individual types of pre-cloned insert modules (plant promoter...

  4. AAV Q&A with Tim Miles

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    Blog Post
    ... an individual to another individual perspective? A: CAP-B10 and CAP-B22 were tested individually in 4... 4 marmosets each. Brain potency for each individual is reported in Figure 4B (see left for image and ...

  5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post
    ...electroporation. To achieve sparse labeling of individual neurons one could reduce the plasmids amounts... of recombinase restricts their expression to individual cells. With this technique, a Neuroscientist ...inducible Cre recombinase (CreERT2), visualizes individual neurons and their synapses in a conditional knockout...for more. In order to analyze the function of individual proteins in neuronal circuits or synaptic remodeling...

  6. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ...screening success? Before you start analyzing individual clones, it’s wise to quickly assess whether a...haystack before going on to generate and screen individual clones. If a clonal line isn’t your end goal,... can determine if it is worthwhile to isolate individual clones from that population. You can additionally... a bulk population and also identify edits in individual clones. The drawback is that you must generate...

  7. Finding Your Perfect Job After University

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    Blog Post
    ...did, however, find it very hard to speak with individuals who had very strong beliefs in traditional medicine... can also get a lot of office politics (many individuals trying to divert more resources towards their...their projects and away from other individuals projects, this can cause conflicts and favoritism, as well...than was anticipated and there can be a glut of individuals with similar skill sets. This is not to say that...

  8. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...instance there are few resources to help get individuals started on the path to internationalization and...development and training in a globalized world where individuals can exchange ideas, network, collaborate, and... supportive and protective environment where individuals are empowered to productively navigate through...Country. Mentees represented a diverse group of individuals with technical and career path needs, and came...

  9. 27 Hot Plasmids from 2016

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    Blog Post
    ... death. The Wu lab library consists of 77,406 individual gRNAs targeting a total of 20,121 genes within...stocks in a single plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et ...these methods can be used to easily modify an individual locus within the genome, they tend to show their... as prior optimizations are required for each individual target species. Indeed, in order to use MAGE,...translational protein fusion would be advantageous when individual protein expression is preferred or required, ...guide RNA transcript and a repair template for an individual tagging event. Commonly used sequences, including...

  10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    Blog Post
    ... ascribed relative to the basic needs of each individual; the  community as a whole has yet to settle ...Instead, I have broken down the tools into the individual features that make each tool unique. The CRISPR...that it is all but impossible to fairly judge individual tools. I believe at this time it is only possible...possible to objectively break the tools into their individual offerings and allow you to select them based ...

  11. Tips for Improving Your Next Manuscript

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    Blog Post
    ... course of the class, the moderator discussed individual sections of a paper, the abstract, figures, introduction...class, we met as a group for sessions covering individual portions of the manuscript. Stephen Blanke, Beronda...generating a quilt with your lab mates, you add the individual figures and results to make the final product...

  12. Hot Plasmids - February 2022

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    Blog Post
    ...SDS-Page gels. A: individually expressed proteins run in separate lanes. B: individually expressed and coexpressed...60, 80 and 100 kD proteins in E. coli, either individually (~20,000 lanes/50 mLs) or through two polycistronic...

  13. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post
    ...Letters of recommendation wisdom  Reach out to 2-3 individuals in your professional circle to vouch for the ...for internship applications. Thus these noble individuals are inundated with requests. The easier you make...the wrong approach. It is very likely you know individuals in your circle that will also benefit from these...

  14. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post
    ...exciting new tool that allows researchers to individually label millions of mammalian cells through lentiviral...ClonTracer library allows a researcher to label individual members of a starting population of cells with...multiplicity of infection (MOI) such that each individual cell receives only one barcode. The abundance...

  15. Engaging with science and society at pgEd

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    Blog Post
    ...make their own decisions about genetics, both as individual consumers of technologies and as societal participants...three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines, and...is to liaise with different organizations and individuals, to identify ways of collaborating, and to organize...

  16. Cloning Mammalian Cells with the Agarose Method

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    Blog Post
    ...antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual colonies by (carefully) holding plates up to ...identified from the plate circling- are then individually punched out, gently sucked up into the pipettor...useful to scientists who wish to rapidly isolate individual cell clones with different expression characteristics...

  17. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post
    ...lines, looking to uncover genetic dependencies of individual tumors, which can lead to new cancer targets ...analysis. Their work revealed that cell lines from individuals of African descent have approximately 20% more...particularly in cancers that are more common in individuals of African descent.Correcting the bias  While...

  18. Data Freedom: The Expansion of Data Sharing in Research Publications

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    Blog Post
    ...of the experiment would require access to each individual video. To PLOS’s credit, the organization subsequently...recording are highly variable and usually left to individual researchers. Yet accurate and comprehensible ...appears to leave the scope of data to be shared to individual authors. Minimally, raw but sufficiently annotated...

  19. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ...After that, the next gates fully depend on your individual experiment. Practical example: lymphocytes in...strategy, I can, of course, also draw multiple individual gates around the populations of interest (Figure...of a quadrant gate, I am free to use multiple individual gates. Note that in this case, square gates were...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post
    ...immunodeficiency syndrome (AIDS) in untreated individuals. Symptoms of AIDS include rapid weight loss and...opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate...CRISPR. CD4+ T-cells were isolated from healthy individuals, expanded, and infected with HIV-1. For two HIV...
Showing: 21 - 40 of 318 results