We narrowed to 167 results for: ipp
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TypeBlog Post...which is limited to the hippocampus. Thus, only neurons signaling from the hippocampus to Broca’s area would...access all the neurons that signaled from the hippocampus to Broca’s area. This could be done by injecting...of a mouse specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent AAV would access ...
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Antibody Guide
TypeCollection...price, and other factors like availability and shipping time. Choose antibodies from companies that are...several to compare, or select one based on price, shipping availability, ease of purchase, or similar factors... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid... 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...this information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples...for 48 hours (to replicate how our viruses are shipped), where they would be exposed to a closed environment...function optimally at -80°C. For samples that are shipped on dry ice, this can affect the pH of the sample... -
Technical Design of a Western Blot
TypeBlog Post...nitrocellulose. PVDF is a sturdy membrane that can be stripped and reprobed multiple times. It doesn’t degrade...washes. It’s less sturdy and not recommended for stripping and reprobing, unless you buy specialty nitrocellulose...nitrocellulose membranes specifically developed to allow stripping. And as a safety note, nitrocellulose is flammable...enzymes, they are more likely to be compatible with stripping and probing. Imaging At the final step of your... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...manipulate RNA species through exon skipping. Previous CRISPR exon-skipping approaches used two guide RNAs ...iPS-derived cortical neurons, dCasRx-mediated exon skipping improved the ratio of pathogenic to non-pathogenic... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping systems and...provide extensive logistics support for export and shipping on dry ice. The Business and Finance Teams continue... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...could have a shipping sprint and all the stories will touch different parts of the shipping code. The following...that the checkout takes the user directly to the shipping step, and no longer takes the user to a terms ... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...studies have found reduced BDNF levels within the hippocampus and frontal cortex during depression, yet there...injecting lentiviral vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior...-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like effects in rats."... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...using a simple, external video recorder and a mic clipped to her shirt. She rolled several long takes, and... videos like this would prove to be difficult. Shipping the gear, and walking everyone through the recording...record into an external recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...by chemical treatment or photobleaching or by stripping out the antibodies, then start over with the next... β1 (white) and anti-GABA-AR β3 (green). (C) Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG...that your target protein is expressed in the hippocampus, or counting the number of puncta in each cell... -
Twenty Years of Sharing Science!
TypeBlog Post...countries. Just eight days into 2024, we've already shipped 2,078 items to 764 scientists at 375 organizations...different countries. It's safe to say we keep our shipping team busy! But they're not alone: to better serve... -
New Viral Vectors - Spring 2025
TypeBlog Post...allow scientists to conduct experiments faster, skipping the preparation steps of production, purification... to the convenience of smaller sizes and quick shipping. We continue to expand the collection to meet ... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their visit for...sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post... Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51....3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61.... -
DNA Service - Cloning Grade DNA
TypeCollection...including the check out and will require separate shipping charges. Where can I find cloning resources or... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post... Their cooler boxes come with a prepaid return shipping label. To return the box, simply peel your address...details on what they accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a ... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post... student at the time, got involved. Along with Philipp Pfingstag, a visiting graduate student from TU ... rapidly. References 1. Kunjapur, Aditya M., Philipp Pfingstag, and Neil C. Thompson. "Gene synthesis... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...coronavirus-related deposits. Our valiant lab and shipping staff agreed to come in and start getting plasmid...plasmid deposits into the collection and to start shipping. They created the best safety protocols they could... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at UCSF. Our team members...Foussard, and Dave Gordon) have been quite busy shipping and sending plasmids to all who request them -...