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  2. Pipetting Protocol

    Type
    Protocol
    ...molecular biology. You’ll use a pipette in many scenarios to accurately dispense small amounts of liquid...

  4. Brain Armamentarium

    Type
    Collection
    ...striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862-PHPeB AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre...striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2...striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214857-PHPeB AiP14361: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3...spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru 214856-PHPeB AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3...spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru 214853-PHPeB AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3...spiny neurons (D2 MSNs) Jonathan Ting Viviana Gradinaru 214852-PHPeB AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3...spiny neurons (D1 MSNs) Jonathan Ting Viviana Gradinaru 214851-PHPeB AiP13987: pAAV-AiE0452h_3xC2-minBG-jRGECO1a-WPRE3...

  5. Brain Initiative Collection

    Type
    Collection
    ... behavior. Long desired by researchers, the revolutionary dynamic picture of the brain generated through...loop for amplifed expression of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with...loop for amplifed expression of the tTA Viviana Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome ...mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAV2 pAAV-mDlx-NLS-mRuby2 An AAV genome ...mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome ...mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2 An AAV genome ...mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2 An AAV genome...

  6. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG... Engineered systemic constructs from Viviana Gradinaru's lab at Caltech. Viral...engineered systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link...NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center (Link opens ...CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV....Deisseroth 117382 hSyn1-eYFP Syn EYFP Control Gradinaru 135630 pAAV-S5E2-dTom-nlsdTom E2 regulatory element...H134R)-eYFP CAG Activator, Cre-dependent ChR2 Gradinaru 135633 pAAV-S5E2-C1V1-eYFP E2 regulatory element...

  7. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S AAV packaging ...controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV packaging...controlled by tTA-TRE amplification system Viviana Gradinaru All Available AAV Packaging Plasmids This table...

  8. Control AAV Preps

    Type
    Collection
    ... 5, rg*, PHP.eB Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV....NLS-mRuby2 Constitutive 1, 2, 8, 9, rg*, PHP.eB Gradinaru 100896 pAAV.GFA104.PI.eGFP.WPRE.bGH GFA104 EGFP...hSyn eYFP Constitutive 2, 5, 8, 9, rg*, PHP.eB Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet ...pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre...pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Gradinaru 137131 pAAV-Ef1a-Coff/Fon-BFP EF1a BFP Flp dependent...

  9. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...proceed with any adjustments to the original order. Turnaround Time Production of a Packaged on Request material...high and we do not expect to meet the general turnaround time, Addgene will notify you by email before...proceed with your order, or not, with a longer turnaround time. Any timing expectations communicated by...by email will supersede the general estimated turnaround time above. After starting viral production, ..., if we determine that we will not meet the turnaround time we described here or by email, Addgene will...

  10. AAV Molecular Tools

    Type
    Collection
    ...Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter...feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible...feedback loop for amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible...Cre-dependent, Tet-inducible expression of EYFP 1 Gradinaru Tools for Affinity Purification These AAV encode...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...Tissue-Specific Gene Disruption in Zebrafish - Leonard Zon Lab Programmable base editing of zebrafish ...origin in zebrafish can be found below: Optimized QF-binary expression system for use in zebrafish - Bret Pearson...localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential ...toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific biotin...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...based on the functional catagories listed below. Narrow down the items available within a category by using...99130 pAAV-mDlx-NLS-mRuby2 Dlx NLS-mRuby2 Control Gradinaru 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control ...Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)...Deisseroth 117382 hSyn1-eYFP Syn EYFP Control Gradinaru 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ..., alpha 8 - IFNAR1 interferon (alpha, beta and omega) receptor 1 AVP, IFN-alpha-REC, IFNAR, IFNBR, IFRC...IFRC IFNAR2 interferon (alpha, beta and omega) receptor 2 IFN-R, IFN-alpha-REC, IFNABR, IFNARB IFNB1 interferon...alpha 8 - IFNAR2 interferon (alpha, beta and omega) receptor 2 IFN-R, IFN-alpha-REC, IFNABR, IFNARB IFNB1 ..., TARC CCL18 chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) AMAC-1, AMAC1, CKb7,...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...subtypes based on shared characteristics and evolutionary similarity. These are grouped into two large...Oost J, Backofen R, Koonin EV. 2015. An updated evolutionary classification of CRISPR-Cas systems. Nat Rev...Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg...

  15. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...labeling observed Publications Ben-Simon Y, Hooper M, Narayan S, Daigle T, Dwivedi D, Way SW, Oster A, Stafford...Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin SM, Lein E, Levi BP, Ting JT, Zeng H...Ojemann JG, Keene CD, Silbergeld DL, Sunkin SM, Gradinaru V, Horwitz GD, Zeng H, Tasic B, Lein ES, Ting ...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia 22 Bernard Weinstein...ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia 20 Bernard Weinstein...ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human...Izaurralde 146577 pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I EIF4G His, HA, V5 Ac5 Parkinson's Elisa Izaurralde...147272 pAc5.1B-lambdaN-HA-DmeIF4Ga-4Ebind-mut-dsRNAres_Q EIF4G His, HA, V5 Ac5 Parkinson's Elisa Izaurralde...147273 pAc5.1B-lambdaN-HA-DmeIF4Ga-del205-234-dsRNAres_Q EIF4G His, HA, V5 Ac5 Parkinson's Elisa Izaurralde...Izaurralde 147274 pAc5.1B-lambdaN-HA-DmeIF4Ga-Y804A-dsRNAres_Q EIF4G1 HA, V5 Ac5 Parkinson's Elisa Izaurralde...

  18. Rett Syndrome

    Type
    Collection
    .... (Link opens in a new window) PMID: 16832102 Krishnaraj et al. 2017. RettBASE: Rett syndrome database...352. (Link opens in a new window) PMID: 16615965 Leonard et al. 2017. Clinical and biological progress over...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...deposited by the Gradinaru lab. For simplicity, in each section we’ll list the Gradinaru capsids first, ... X., Luo, Y., Einarsson, P. H., Greenbaum, A., Jang, M. J., Deverman, B. E., & Gradinaru, V. (2020). Multiplexed...capsids in our repository are deposited by the Gradinaru lab. You can visit our Caltech Systemic Capsids...not otherwise attributed were deposited by the Gradinaru lab.  AAV-PHP.B was the first systemic capsid ...capsid developed and deposited by the Gradinaru lab (Deverman et al., 2016). Compared to AAV9, it effectively...specificity than AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX will...

  20. Plasmids for Stem Cell Research

    Type
    Collection
    ...beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells induced Beta Cells Adenoviral Mouse Long-...pancreatic beta cells generated by lineage conversion of acinar cells. Nature Biotechnology. 2014 Dec;32(12):1223...
Showing: 21 - 40 of 275 results