... and Negative Selection for Plasmid Cloning by Jennifer Tsang There are many strategies for finding your...Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi...Precision and Flexibility to CRISPR Editing by Jennifer Tsang Prime editing is a new CRISPR technique... Jennifer Tsang...

...portion of optogenetics (though it does require a significant amount of equipment, most of which is relatively...point; the fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency...stimulation, the rate of movement of the animal significantly increases. Before you get your experiment started...demands of using these wireless devices are significantly higher than standard “wired” optogenetics but...

...were less significant than when non-targeting guides are used as controls. Less significant p-values lead...guides and may more accurately determine the significance of hits in non-growth screens. Off-target cutting...of on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes...

...Addgene, I was about to have a check-in with my boss Jennifer. I had finally found my footing with the company... for what we thought would be a couple months. Jennifer and I spent about an hour that morning scrambling...know cameras, but we needed someone else. Design: Jennifer thankfully volunteered, and we decided to focus...action would be to use an iPad to shoot.  Build: Jennifer created a mobile camera area that included iPad...

...for options if the recombinant band makes up a significant proportion of the DNA pool. Last Update: August...taking place during electroporation. Arcing would manifest as a loud pop often accompanied by some light ... off entirely if the column or reagents are significantly overloaded. If you find that your pellet outstrips...

...libraries also include 2,400 gRNAs without any significant hit across the E. coli genome as a negative control... fold and >3,800 fold in human cells with no significant reduction in editing efficiency. Read the paper... Jennifer Tsang...

... of twofold or greater with good statistical significance (Taylor, 2017). With the right conditions and...multiplexing is quite difficult and requires significant optimization to ensure the results are correct...multiplexing your reference gene set can save significant plate space and may be worth the initial effort...statistical analysis to determine if there are any significant differences between your experimental values ...

...annotate genetic perturbations that may play significant roles in cancer pathogenesis. Their work culminated...mutations were found to be “neutral,” having no significant effect on cell viability compared to a wild-type...Approximately ~22% of the mutations, however, significantly increased cell viability, and these “activators...

...an attempt to present only the most novel or significant findings, and combined with powerful cognitive...QRPs) can quickly lead us to find statistically significant results that are not robust. Sampling noise There...are too easy to overlook when presented with a significant result that may be the result of noise, p-hacking...

...enzymes outlined below as well. Cas12a also has significant therapeutic promise due to the fact that it is...target sequence Similar to Cas12, it is also significantly smaller in size than traditional Cas9. Another... not display collateral RNA cleavage and is significantly less toxic to mammalian cells than Cas13 (likely...

...noncovalent dimers. This property of EGFP can lead to significant artifacts. If you're using green fluorescent ... Fusion of an FP to a transmembrane protein significantly increases the effective concentration of the...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

...applications. As individual libraries can vary significantly, it’s important that we have an amplification...overtake larger plasmids, if the library varies significantly in plasmid size). On plates, each transformed...ensure that your conditions did not result in significant recombination. If you do see recombination, we...

...caudal NST. (A-C) Low-magnification images showing viral spread. (D-F)  High-magnification images immuno-stained...minimum n = 2, and well labeled high- and low-magnification images demonstrating vector performance) will...

...allows you to physically magnify a sample so you don’t have to optically magnify quite so much. Created ...Klimas A, Gallagher BR, Wijesekara P, et al (2023) Magnify is a universal molecular anchoring strategy for...

...enzyme can lead to off targets and result in significant, negative effects in RNA targeting experiments...non-specific activity. Its major advantage is the significantly reduced amount of off-target toxicity compared...using Prime editing alongside some creative (and significant!) protein engineering. They combined Prime editing...

...you! Many thanks to our Guest Blogger, Jennifer Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research ...This post was contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in...

...development happening at Addgene under the hood. A significant portion of our development may not be visible... been updated in several years) or they make significant changes that make it incompatible with our software...software from Python 2.7 to Python 3.5. There are significant changes to the language and it took us a total...

... the cytoplasm? Or more troubling, what if a significant fraction of the fusion protein molecules fail...resident proteins within these compartments undergo significant post-translational modifications including glycosylation...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. DNA dissolved in water is ...

...vastly expanded axolotl research. One of the most significant advances is the sequencing of the entire axolotl...research providing insight into some of the most significant and fundamental biological processes. Many of...widespread utilization, Arabidopsis has made significant contributions to understanding plant and crop...