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We narrowed to 69 results for: pcas9

Showing: 21 - 40 of 69 results

  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...genomes that lack the 3’-NGG PAM sites favored by SpCas9. Since Cpf1 doesn’t require a tracrRNA, crRNA guides... Cpf1 and its guide RNAs are smaller than their SpCas9 counterparts, they will also be easier to deliver...only slightly lower than that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs...cleavage at 1-12 off-target sites; in contrast, SpCas9 may cleave at ~90 sites, according to Kim et al...off-target activity than that previously observed with SpCas9. Kleinstiver et al. suggest that AsCpf1’s off-target...

  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...85174  pBK-YE1-BE3  SpCas9 (NGG)  Editing window ~2 nt  85175  pBK-EE-BE3  SpCas9 (NGG)  Editing window...85176  pBK-YE2-BE3  SpCas9 (NGG)  Editing window ~2 nt  85177  pBK-YEE-BE3  SpCas9 (NGG)  Lower on-target...Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition (Human ...function or cause cellular growth delays. With eSpCas9(1.1) and coselection, Agudelo et al. achieved editing... deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as 1...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few spCas9s which have been evolved...of Maya Kostman. The most commonly used Cas, spCas9, has gone through multiple rounds of optimization...

  5. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation...The PAM Requirement and Expanding CRISPR Beyond SpCas9 Day 8: Repair mechanisms: CRISPR 101: Non-homologous...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...getting the desired features and cutting (note: spCas9 isn’t always the winner!).   Fig. 3 – Comparison...The PAM Requirement and Expanding CRISPR Beyond SpCas9 More resources from the Innovative Genomics Institute...

  7. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...the fact that it is smaller than the traditional SpCas9 enzyme (about a third of the size) and does not...cleave RNA through the same mechanism as traditional spCas9. If you are looking to modify RNA, Cas9 just won...The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources on the Addgene Website...

  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome engineering tool... species, and has many applications. That said, SpCas9 is not the only game in town, and other Cas proteins... can circumvent the limitations associated with SpCas9. Cas13a (previously referred to as C2c2), has several...

  10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...period of time. High fidelity Cas9s such as eSpCas9 and SpCas9-HF should be explored to reduce off-target...mutation in dystrophin exon 23. Long et al. used SpCas9 in conjunction with AAV9, whereas Nelson et al....

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...capacity of a locus. When using S. pyogenes Cas9 (SpCas9), potential target sites are both [5’-20nt-NGG]...editing, there are usually few potential gRNAs. While SpCas9, with a PAM preference of NGG, is still the most...gRNAs that are likely to be active, not only for SpCas9 but also for some other Cas enzymes. It seems to...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...used for each gene. At the moment, 13 full genome SpCas9 knockout libraries designed by different academic...Library, for example related to strategies beyond SpCas9, or species not currently included in Green Listed...libraries. As of now I have focused on human and mouse SpCas9 knockout libraries, but there are of course great...

  13. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... makes it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a particularly prominent...Institute to provide lentiviruses for delivery of SpCas9 and Cas9 nickase for genome editing. You can also...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length...sequence, tRNAs, multiple individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts...

  16. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...well characterized than the conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes...been shown to exhibit more limited activity than SpCas9 (2). In many cases this is due both to lower relative...

  18. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the ICE software...configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency (percentage...

  20. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as 1...available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site Chen et al., 2023 Td-CBE...
Showing: 21 - 40 of 69 results