Skip to main content

We narrowed to 119 results for: sag

Showing: 21 - 40 of 119 results
  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...initial paper, we used MUCICAT to optimize the gene dosage of glucose dehydrogenase and generated a strain...higher cargo copy numbers. (D) The effect of gene dosage on N-acetylglucosamine concentration. Image from...construct a strain library with different gene dosages to facilitate swift optimization of strains. Multiple...
  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... culture post thaw. In general, the higher the passage number of a culture the lower the transfection ...transduction efficiencies. Cultures should only be passaged 20-30 times before discarding and thawing a fresh...Type Culture Collection and freeze as many early passage vials as possible. One problem that often plagues...
  3. TALEN Plasmids and Kits

    Type
    Collection
    ...included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI domains....
  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...SERPINI1 Dementia Joan Massague 58260 pBabe-Neuroserpin oloop SERPINI1 Dementia Joan Massague 58261 pBabe-FLAG-Neuroserpin...Dementia Joan Massague 58262 pBabe-Puro-FLAG-Neuroserpin oloop SERPINI1 Flag Dementia Joan Massague 59416 pBMN-YFP-Parkin...
  5. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...with. My inbox currently stands at about 65,000 messages and dealing with email can be half of my day sometimes...translation of results to companies.” Take home message Starting a lab can be a challenging process. Each...
  6. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...like cell phone networks. Protein kinases deliver messages to the next members of their pathways, but where...their signals last all impact transduction of the message. To study signaling pathways, scientists frequently...
  7. Tips for Technical Support Calls

    Type
    Blog Post
    ...your initial query. This can reduce the number of messages in our thread or remove a good 5 minutes from ...great pick-me-up when things get crazy. Take home message Answering customer service questions is a delicate...
  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...separate non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged...
  9. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...Results of Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling...downstream editing. Overall, the real take-home message thus far, based on survey respondents "Final Verdict...
  10. CRISPR Antimicrobials

    Type
    Blog Post
    ...resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria, allowing them to...help educate the public about proper antibiotic usage. Although these steps are laudable, they likely ...
  11. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...discordance plot (right panel) shows the level of disagreement between the non-edited wild type (control) and...base-by-base, the average amount of signal that disagrees with the reference sequence derived from the control...
  12. Viral Production at Addgene

    Type
    Blog Post
    ...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...
  13. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...advocate. This is the person whose job it is to disagree with the group. They are the ones who ask for ...While the best devil’s advocate is one who truly disagrees with the group (because they will fight more adamantly...
  14. A Control for All Seasons

    Type
    Blog Post
    ...Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications, Jan. 2011, pp. 6–12. Crossref, doi...Histochemistry & Cytochemistry, vol. 62, no. 10, SAGE Publications, July 2014, pp. 693–697. Crossref, ...
  15. Adenovirus Guide

    Type
    Guide
    ...biology, recombinant adenovirus, and adenovirus usage in gene therapy . Viruses, 13 (12), 2502. https:...
Showing: 21 - 40 of 119 results