Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 34 results for: sequence data Fluorescent Protein Guide

Showing: 21 - 34 of 34 results

  1. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    ...encodes for a small Cas protein (40-70 kDa), which is half the size of other Cas proteins found in the so-called...cleave a targeted sequence of ssDNA. Unlike Cas9, Cas14 does not require a PAM sequence. In addition to ... of a viral sequences, for instance, the enzymes are activated and can cleave fluorescent probes of dsDNA... and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay. By adding... of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But what is the function ... types of nucleic acids and required a certain sequence specificity in the middle of the gRNA to be activated...Find the Cas14 plasmids here Checkout our CRISPR guide page Browse the CRISPR collection ...

  2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...reagents to cells: a guide RNA, Cas9 protein, and a donor DNA that contains the tag sequence flanked by homology...approaches to study protein interactions is the pull-down assay, wherein a protein of interest can be ...IP/MS, a target protein is first immunoprecipitated - along with its associated protein complexes - from...using antibodies against the target protein. The captured protein complexes are then analysed by mass ...interacting proteins. Similarly, in ChIP-seq assays, chromatin fragments that are bound by a protein of interest...high-throughput sequencing to identify genome-wide binding patterns of the target protein. Both IP/MS and...pre-validated antibody for each target protein. To study 20,000 human proteins, this would be time-consuming, ...

  3. Deep Dive: qPCR

    Type
    Blog Post
    ...TipđŸ”„ The ABI machines automatically record fluorescent data on all channels - helpful if you forget to.... al No Raw Data researcher defines log-linear phase Liu and Saint No Raw Data Reference and...Reporting your data  Once you’ve done the work to produce excellent and reliable qPCR data, you’ll of course... be used as a proxy measure for differences in protein expression. (If you're just getting started, check...fluorogenic primers Low Single self-quenching fluorescent molecule connected to a primer  No Yes ...curve. If relying on previously-generated validation data, be aware that different machines, samples, and ...product on an agarose gel, then cutting out and sequencing the band for verification. Primer dimers will...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...along with the guide RNA. For most applications, we recommend using Cas9 with the single guide RNA (sgRNA)...target site sequence (20bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found...target site sequence (30bp) and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 ...PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9...2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461;...nickase) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...stemloop - Contains BbsI sites for insertion of guide sequences Addgene plasmids #61425 - #61427 are intended...

  5. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, ...SnapGene file for the sequences listed. You can then analyze the sequence in your sequence viewer of choice...SnapGene’s sequence viewer software and extensive feature library, our updated plasmid and sequence displays...differ by only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features, ... our sequence analyzer will allow you to dive into the details. Click on the “View all sequences” button...find all available sequence information from Addgene depositors and our own sequencing results: On this ...paste functionality. Sequence Similar to the plasmid maps, the linear sequences hosted here are now ...

  6. Worm Expression Resources

    Type
    Collection
    ... includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...Genomes - Cold Spring Harbor's guide to RNAi in C. elegans WormAtlas - A database featuring behavioral and ... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...worm ( C. elegans, C. briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene...ordered individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology....Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...step: protein degradation, incomplete lysing/insoluble proteins, and protein aggregation. Protein degradation...isolating proteins from a specific compartment. If your proteins are nuclear- or DNA-binding proteins, you ...control protein (if available) and note in analysis Plasmid-based proteins: plasmid sequence not accurate...two types of gradients. Protein gradients use dilutions of your sample or protein of interest to understand...  Figure 1: A protein gradient. The density of the bands, which increase as protein concentration increases...in order to release the proteins from their binding. If you’re seeing protein aggregation, you may want...you need very accurate protein measurements, consider using an unstained protein ladder, which will run...

  8. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ... from Addgene’s sequencing reactions (‘Addgene Sequences’).  These sequences are provided in FASTA format... your research! Sequence information: Depositors may provide full or partial sequence information for ...be made available as ‘Depositor Sequences’. Additional partial sequence information is also available from... such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can find...most of these pages, we've written this post to guide you through them and make the best use of all the...auto-generate a plasmid map based off of the plasmid sequence and feature information given by our depositors...example) and can be analyzed using Addgene’s Analyze Sequence tool. Excellent, how can I check if the plasmid...

  9. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...unreliability: cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs...technology, it is important to show through qualitative data that they are a long-term solution to reproducibility...atlases/literature and is unrelated to the target protein, we categorize the antibody as having non-specific...atlas will enable scientists to have an intuitive guide for checking their staining and neuroanatomy without...you are interested to get involved by submitting data, sending antibodies, and/or working with Dr. Wu ... dedicated to creating a public and accessible database for low-cost, high-quality antibodies for IHC....

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc...BBB via interactions with a similar protein called LY6C1. This protein is expressed in both BALB/cJ mice...psst
 don't forget to submit your validation data to our Data Hub once you’re finished!) Good luck and happy...exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While... miRNA target sites, and recombination target sequences, denoted by triangles] facilitate tissue or cell...option for your experiment? Look it up in Addgene’s Data Hub and the CLOVER repository of published systemic... it, cross the BBB through an endothelial cell protein called LY6A (Hordeaux et al., 2019; Huang et al...

  11. Immunology Research Plasmids and Resources

    Type
    Collection
    ... domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2,...morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic protein 7 OP...TYROBP TYRO protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I...assay data for translational and clinical research. Sci Data. Feb 27;5:180015. doi: 10.1038/sdata.2018.15... You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories...shock 70kDa protein 1B FLJ54328, HSP70-1B, HSP70-2, HSPA1A HSPA1L heat shock 70kDa protein 1-like HSP70...

  12. Antibody Guide

    Type
    Guide
    ...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...

  13. Molecular Biology Reference

    Type
    Guide
    ...Antibiotics DNA Sequencing Sanger Sequencing Next-generation Sequencing Long-read Sequencing Reference Tables...of interest. Sequencing technologies like Sanger sequencing and next-generation sequencing (NGS) take advantage...Sanger sequencing, sometimes referred to as chain-termination sequencing or dideoxy sequencing (Figure...the instructions to make every protein within an organism. Before proteins can be translated, the DNA is...RNA (mRNA), which is then translated into proteins. Proteins are made up of amino acids. Each amino acid...control of a non-bacterial promoter or a fluorescent protein to select or sort the cells by visualization...single-stranded DNA sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid...

  14. Optogenetics Guide

    Type
    Guide
    ... Science Guides Optogenetics Guide Optogenetics Guide Jump To: Optogenetics Guide Microbial Opsins...release proteins from cellular membranes using light. light-induced protein dissociation using protein A fragments...Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains...photoreceptors to create protein systems controlled by light. These "photoswitchable" proteins offer exquisite ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...Inhibits protein function by reversibly sequestering targets into large, optically assembled protein architectures...transcription using engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage...
Showing: 21 - 34 of 34 results