We narrowed to 32 results for: shRNA construct

...integrated by the virus. If an oncogene overexpression construct is being delivered, that is a higher risk cargo... GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, this ...

...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...such as microRNAs (miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger...

...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...promoters, enhancers and untranslated regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing...introns Anthony Leung Return to Top Expression Constructs Luciferase driven by a strong constitutive promoter...luciferase). Feng Zhang Return to Top Reporter Constructs Already know what gene or regulatory element ...reporter for? Browse or use the search box to find a construct that contains your regulatory element of interest...rapamycin-inducible interaction. nLuc and cLuc : Constructs for split firefly luciferase complementation ...and Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag...

...., 2008). 2. Transient expression of transgenes/shRNAs If you want your cells to express a protein or ...plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change...

...the different vectors used, lentiviral vector constructs have proven very useful due to their ability ...lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors...

...Howarth lab created the MBPx-SpyCatcher construct. This construct is used to produce a maltose binding protein...However, the laborious construction of repair templates and guide RNA constructs, as well as the difficult... Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed through a large-scale... that results in the creation of many E. coli constructs with greatly varied levels of expression. The...can be used to easily create protein expression constructs fused with epitopes or fluorophores, generate...platform for generating both simple and complex DNA constructs Have you ever wished you could design a gene...overexpression experiment, generate the necessary constructs, and complete screening in just 3 days? Thanks...

...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done...linearized with the restriction enzyme PmeI . This construct is then transformed into AdEasier-1 cells (Addgene...linearized with PacI to create a linear dsDNA construct flanked by ITRs. Cells from the packaging HEK293...expressing E1 are transfected with the linearized construct, and rAdV vectors can be harvested about 7–10 ...purposes. The pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene...adding 2.7 kb of packaging space. However, these constructs must be transfected into HER911 E4+ cells for...Ad2 are the most commonly used for rAdV vector construction, due to their high gene transfer efficiency....

...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging capacity...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done...envelope, and transfer plasmids, and that resulting construct is packaged into a viral particle, it is not possible...

...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging capacity...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done...include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and other disease-associated...envelope, and transfer plasmids, and the resulting construct is packaged into a viral particle, it is not possible...

...interested in. Self-replicate — Once you have constructed a plasmid, you can easily make an endless number...frequently accomplished through expression of an shRNA targeting the mRNA of the gene of interest. These...that can drive expression of short RNAs. Mammalian shRNA Tools Genome Engineering Plasmids Used to target...

...deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions...Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid can be done... most commonly used serotype for rAAV vector construction, as it was the first to be well-characterized...host cells. The full-length transgene is later reconstructed within the cell via different mechanisms. One... they form concatemers and splice together, reconstructing the full-length transgene that can then be ...

...Parkinson's David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335...pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1) NR4A2 GFP CMV Parkinson's Glenn Schafe 233274 pAAV-CMV-GFP-pA U6 shRNA (Rat ...ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin...28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP CMV ALS...28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP CMV ALS...28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP CMV ALS...28201 tdp43-EGFP construct8 TARDBP GFP CMV ALS Zuoshang Xu 28202 tdp43-EGFP construct9 TARDBP GFP CMV ALS...