We narrowed to 183 results for: tTA

...irreversibly with a small substrate ligand motif attached to a fluorophore (Figure 1b). Engineered ligases...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure...bioorthogonal chemical reactions (i.e., click chemistry) to attach a fluorophore at the specific site of incorporation... the speed and selectivity of the fluorophore attachment, the brightness of the resulting conjugate, and...

...cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt fox1 (a2bp1l) OZ519 and OZ520 OPEN OPEN...aCGTTCACTCggtaaTGGGCCGACt Atg7 OZ557 and OZ558 OPEN OPEN tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg...gCTCATCTCCACCGAGCTGGAGAAg atp2c1 OZ567 and OZ568 CODA CODA tTACCTCCACGATCTTGCTGATGGAg dio2 OZ569 and OZ570 CODA OPEN aAGCTGCTCCAAACTCTAAGGTGGTg...gTCCAGCAGCGGTGAGGATGAAGACg unc119b OZ581 and OZ582 CODA CODA tTGCTGCCGCGGATGCTGTGGCGGTTa cx52.9 OZ583 and OZ584 OPEN OPEN cTTCATCTTCCGCATGCTGGTGCTg...

...specifically at ‘TTAA’ sites randomly dispersed in the genome. The predicted frequency of ‘TTAA’ in the genome...sequence from the transposon vector to one of many TTAA sequences distributed throughout the genome. We ...donor-transposon (carrying your cargo of interest) into random ‘TTAA’ sites throughout the genome. When using piggyBac...

...Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green algae (Soo et al., 2015...parasite that enters fish through its gills. It attaches there until they mature and changes sex from male..."Tongue replacement in a marine fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." ...

...characterized Argonaute from Thermus thermophilus (TtAgo), showing that this protein could cleave DNA in ...Unfortunately, Swarts et al. were unable to show TtAgo-mediated cleavage in mammalian cells, likely due...al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute. They ...

...to our guest blogger Brittany L. Uhlorn from the University of Arizona. Brittany L. Uhlorn is a fifth ...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps...

...transposase. In rtTA-cell lines, it is better to first engineer the cell line with rtTA, then integrate...can be used directly in transient transfection in rtTA+ cell lines (such as HEK293-TETOn cells), or they...

...even decades after it is initially isolated. Regrettably this is not usually the case if you obtain a ...Rebecca, and Bahni Turpin. The immortal life of Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold...Walter A. Nelson-Rees, and Grover M. Hutchins. "Henrietta Lacks, HeLa cells, and cell culture contamination...

...looper moth (Potter et al., 1976). Its target site is TTAA and unlike other transposons, it does not leave ... its preferred target integration site: TNA(C/G)TTATAA(G/C)TNA. Tol2 can deliver 10-11 kB to mammalian...have a specific integration target site, such as TTAA for Sleeping Beauty, they can insert at any number...

... strategies include using engineered ligases to attach a ligand to a peptide tag on your protein of interest...standard confocal microscopy. Image adapted from (Bottanelli et al., 2016) under CC-BY license. Combining...experiments!   References and resources References Bottanelli, F., Kromann, E. B., Allgeyer, E. S., Erdmann...

...2. The Immortal Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the African American...

...virion surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence... of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking...

...fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome to Montana...

...integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed information about...promoter integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry...

...lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve ...serve as controls in a variety of experiments, while rtTA can be used to activate expression of a gene under...

...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol...

...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

...detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody is done through...direct ELISAs, an antigen or protein of interest is attached to the well of a 96-well plate. A conjugated primary...recognize the protein in its native conformation. Attachment of the antibody to the beads is typically done...

...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...bacterium to make a small “bridge” or sex pilus that attaches to the recipient cell drawing it close. Once in...

...separate them. Most proteins are not likely to remain attached to the DNA throughout the sample processing and...sample may need more or less cross-linking to firmly attach the proteins to the DNA, depending on how tightly...