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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 363 results for: tac

Showing: 21 - 40 of 363 results

  2. AAV Molecular Tools

    Type
    Collection
    ...cell ablation. 2 Jessell , Azim 45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, ..., bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. 5 Shah , Wells Genome...

  4. mTOR Pathway

    Type
    Collection
    ...Cancer Pathway ORF Kit Ras Pathway p53 Pathway Tackling Cancers’ Drug Resistance with a New Screening ...Resources Cancer Pathway ORF Kit (Sabatini & Wood) Tackling Cancers’ Drug Resistance with a New Screening ...

  5. Viral Production

    Type
    Collection
    ...the facility that produced the viral vector lot. Contact us to determine which facility produced your viral...experiments were performed on your lot, please contact us . Full sequencing of the Viral Genome Next-generation...

  7. COVID-19 Resources

    Type
    Collection
    ...protein, activating its membrane fusion function.( 5 ) TACE - (ADAM17) a metalloprotease that cleaves hACE2,... of Rather than Adjacent to the Fusion Peptide . TACE - Heurich A, et al , 2014, J Virol. TMPRSS2 and ...

  8. Rett Syndrome

    Type
    Collection
    ...the labs in which they were created. Find their contact information by following the link to their laboratory...

  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ... L, Lin JK, Wang M, Zhou L, Xu XH, Sun YG. 2019. Tac1-Expressing Neurons in the Periaqueductal Gray Facilitate...Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in the Periaqueductal Gray Facilitate...

  10. Retrograde AAV viral preps

    Type
    Collection
    ...pAAV-CKIIa-stGtACR2-FusionRed CamKII Inhibitor Optogenetics Yizhar 105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed...

  11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein...cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells ... Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized cells  Cy5NTAc 43 34-62 YFP   ...   Lumio Red Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly His  65 52-94 Plasma membrane protein, permeabilized...resulting in site-specific incorporation of the attached fluorophore. However, since tRNA suppression competes...cell membranes, thus enabling FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector...permeabilized cells  Cy5NTAc 59 47-85 a R0 is the donor/acceptor (D/A) distance at which 50% FRET...

  12. Brain Initiative Collection

    Type
    Collection
    ...AAV9 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and ...

  13. Plasmid Cloning by PCR

    Type
    Blog Post
    ...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

  14. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...improve resolution, use a stacking gel on top of the resolving gel. A stacking gel typically has a different...protein’s secondary and tertiary structures remain intact and the protein is separated through a matrix by...of the proteins in a sample migrate through the stacking gel at the same pace and enter the resolving gel... causing proteins to migrate out of the gel and attach to the membrane.  In a wet transfer system the ...Figure 2: The western blot dry apparatus includes a stack of filter paper, a copper anode/iblot gel matrix...

  15. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...gills. It attaches there until they mature and changes sex from male to female. Then, it detaches from the...Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green algae (Soo et al., 2015...those linked to neurotransmitter activity and geotactic behavior (Biron et al., 2005).   References Biron...fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert...

  16. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...endogenous molecular target, meaning the dye will be attached to the target through binding of the molecular...encoded tag is required. These dyes can also be attached to an antibody to detect various targets, such...or glycans. Fluorophores can also be covalently attached to self-labeling proteins. Some common examples...covalently reacts with a small substrate ligand motif attached to a fluorophore. When paired with a protein of...higher specificity, increased versatility in probe attachment, and compatibility with far-red and near-infrared...Isothiocyanate-conjugated Antibody. Salari, M., Bitounis, D., Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, ...

  17. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ... know better. Networking is not about how many contacts you have; it’s about building genuine relationships...authenticity and not discomfort: Invite the new contact to connect on LinkedIn and always include a personal...profile link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters...postdoc association. It was 25 years since our last contact, but we were delighted to be reconnected.  Science... small community and you will come across your contacts over the years in many different ways. This leads...

  18. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Research tackling questions in the fields of Cancer, Inflammation and Immunity, as well as various combinations...Researchers and Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary.... to understand cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few...are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis The conference...d like to share with the scientific community, contact [email protected] to find out more about our ...

  19. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...sequence. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites...canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does not occur naturally...

  20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ... your plasmids back to us. Our scientists will contact you if any issues arise during the QC process. ...collection that is greater than 75 plasmids, please contact us at [email protected]. These deposits are considered...strain in the “Growth Strain Other” field, and contact Addgene. We may require that you send your samples...in bacteria, select “yes” in the drop-down and contact Addgene. We can only accept plasmids that can be...to prepare and ship the samples to Addgene and contact your technology transfer office about the MTA. ...
Showing: 21 - 40 of 363 results