...which can be toxic in living cells. BioID was derived from biotin ligase and uses far less toxic biotin for...pair of enzymes that overcome both the speed and toxicity limitations of APEX2 and BioID. In the Ting Lab...describe TurboID and miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as APEX2. What...

...important to note that the biotin-phenol reagent can be toxic to living cells thus APEX is not suitable for organoid...APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity labeling  TurboID and miniTurbo were developed...rapidly label proteins, requires H2O2 which can be toxic to living cells. To circumvent these issues, the...biotin labeling in 10 minutes without any issues in toxicity. TurboID’s signal is significantly greater than...

...recognized by effector cells of the immune system - cytotoxic T lymphocytes (CTLs), natural killer (NK) cells...programmed cell death ligand (PD-L1) that inhibits the cytotoxic activity of CTLs and NK cells. These cells would...immunotherapy. Using activated DCs ex vivo to generate cytotoxic effector T cells, and targeting specific DC subsets... targets like CD19 on tumor cells and exhibit cytotoxic activity. CAR-T cells have consistently shown ...

...live-cell imaging, the use of dyes that exhibit low toxicity and high photostability is crucial. These properties...dye's ability to enter cells without causing undue toxicity. A prominent example is DAPI, a dye that binds...in live cells can sometimes result in cellular toxicity due to the permeation process. Advanced technologies...of fluorescently tagged nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5)...

...transduction) targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate...cells showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency of biallelic mutations... by nature transient, which may lower nuclease toxicity or the potential for off-target effects. Third...

...replication in mammalian cells, for example, and a toxin that kills bacteria may not have any discernable... a cassette that will make cells resistant to a toxin), whereas negative selection would be the picking... a cassette that will make cells sensitive to a toxin). In the table below we focus on positive selection...

...core of this tool is the endoprotease Botulinum Neurotoxin B (BoNT/B), which cleaves the synaptic protein... to SspB, brings together the split botulinum neurotoxin halves to enable VAMP2 cleavage, and suppresses...photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin were also created. The depositors demonstrated...

...Long-term light exposure can also lead to cellular phototoxicity, and experimental success can be affected by...-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple subcellular...imaging, especially where photobleaching and phototoxicity are a concern, these constructs come in very...

...starting knock-in experiments. Test dTAG molecule toxicity in new cell lines Treat parental cells (or cells...biological assay of choice to ensure that there are no toxicities at the doses used to evaluate degradation of ...have evaluated to date, we observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures...

...Supplemental Information New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ...membrane, nucleus, etc.) are also available Non-toxic to living cells To learn more about this system,...

... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy...

... handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can transduce both...non-dividing cells with a low immune response and low toxicity. Although recombinant AAV does not integrate into...

... cool things about amp is that it's exclusively toxic to dividing cells. The other antibiotics listed ...across systems and species. The cost? Zeocin is genotoxic when not 100% inhibited, meaning it will induce...there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy et al...

...genetic tools to the brain because of its low cytotoxicity, low immunogenicity, and the long-term expression...of FPs can trigger protein aggregation which is toxic for the cells and affects the labeling of small ...fluorescence. Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP...

...signs of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin...

...antigens found in the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs...tumor site. ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...

...severing and has very low dark-state activity and toxicity. All of that means this light-powered sword truly... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks interested in ...

...low levels to reduce toxicity; See PI page for other recombinases that are less toxic in Drosophila hsp70...recombination is a very useful tool, Cre expression can be toxic in some systems, notably Drosophila. Read Addgene's...

...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...

...particle) and toxicity of the gene product (i.e., if the gene encoded by the transfer plasmid is toxic to mammalian...