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  1. General Transfection

    Type
    Protocol
    ...µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

  2. Lentivirus Production

    Type
    Protocol
    ... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...efficiency of viral infection. However, polybrene is toxic to some cell lines. In these cell lines, substitute... media 24 hours after infection. TIP: If viral toxicity is observed in your cell line, you may decrease...

  7. AAV Production in HEK293 Cells

    Type
    Protocol
    ...post transfection. Changing the media can reduce toxicity associated with PEI and allow for the addition...
Showing: 1 - 7 of 7 results