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  1. Viral Production

    Type
    Collection
    ...gram-negative bacterial endotoxin is ensured to be less than 5 endotoxin units per mL. The endotoxin levels are determined...recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector preparations can ...large animal studies. Endotoxin contamination is minimized by using an endotoxin-free plasmid purification... determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity...addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources Browse...

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...RIFLE, TAL LTA lymphotoxin alpha (TNF superfamily, member 1) LT, TNFB, TNFSF1 LTB lymphotoxin beta (TNF superfamily...member 1 CD94 LGMN legumain AEP, LGMN1, PRSC1 LTA lymphotoxin alpha (TNF superfamily, member 1) LT, TNFB, TNFSF1...SHP-1, SHP-1L, SHP1 RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein..., TC-25, p21-Rac1 RAC2 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein...EN-7, Gx, HSPC022 RAC3 ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein... RNase A family, 2 (liver, eosinophil-derived neurotoxin) EDN, RNS2 ROBO1 roundabout, axon guidance receptor...12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) CLMF, IL-...

  3. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness...PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide...

  4. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Supplemental Information New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ...membrane, nucleus, etc.) are also available Non-toxic to living cells To learn more about this system,...

  5. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can transduce both...non-dividing cells with a low immune response and low toxicity. Although recombinant AAV does not integrate into...

  6. Cre-lox system

    Type
    Collection
    ...low levels to reduce toxicity; See PI page for other recombinases that are less toxic in Drosophila hsp70...recombination is a very useful tool, Cre expression can be toxic in some systems, notably Drosophila. Read Addgene's...

  7. Lentivirus Plasmids

    Type
    Collection
    ...particle) and toxicity of the gene product (i.e., if the gene encoded by the transfer plasmid is toxic to mammalian...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...tagRFP Ebert pSAG1::CAS9-U6::sgUPRT 54467 Other/Toxoplasma gondii none, Q5 mutagenesis yes, cut S. pyogenes...pyogenes Sibley pU6-Universal 52694 Other/Toxoplasma gondii BsaI yes, cut S. pyogenes Lourido pRGE31 50929...

  10. AAV Molecular Tools

    Type
    Collection
    ...Cre-dependent Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation...

  12. Microbiology Resources

    Type
    Collection
    ...Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii Trypanosoma sp. Plasmids for Viruses Species...

  13. Ras Pathway

    Type
    Collection
    ...homolog RAC RAC1 RAC2 RAC3 Ras-related C3 botulinum toxin substrate (rho family, small GTP binding protein...

  14. CRISPR Guide

    Type
    Collection
    ...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...

  15. Tetracycline Inducible Expression

    Type
    Collection
    ...systems. Dox also has good tissue distribution, low toxicity, a known half-life (24 hours), and is compararably...

  17. Plan Your Experiment

    Type
    Collection
    ...dividing and non-dividing cells AAV is the least toxic method for in vivo viral delivery RNA delivery of...

  18. Bacterial Expression Systems

    Type
    Collection
    ...which may aid disulfide bond formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188...

  19. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...2 plasmid) Knockout Human Moffat 3rd 4 70,948 Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A...
Showing: 1 - 20 of 22 results