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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 174 results for: tub

Showing: 21 - 40 of 174 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...Streptococcus pneumoniae. Robert Husson lab's M. tuberculosis CRISPRi plasmids A group from the Husson lab... used CRISPRi to study essential genes in M. tuberculosis. dCas9 and a gRNA are expressed on pRH2502 and... (Singh et al. 2016). Sarah Fortune's lab M. tuberculosis CRISPRi plasmids Noting that the more common... for transcriptional silencing Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition to ...Investigating essential gene function inMycobacterium tuberculosisusing an efficient CRISPR interference system. Nucleic...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...our guest blogger Kutubuddin Molla from the Pennsylvania State University Kutubuddin Molla is an Ag-biotechnologist...This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State... Institute, Cuttack, Odisha, India. Currently Kutubuddin is a Fulbright Visiting Scholar at the Pennsylvania...

  3. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...way! Many thanks to guest blogger Kaustubh Kishor Jadhav! Kaustubh Kishor Jadhav, MSc is a Research Assistant...This post was contributed by guest blogger, Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute...lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain in the...

  4. Clear and Accessible Writing

    Type
    Blog Post
    ...used to convey the difference between a gradient tube before and after ultracentrifugation so that a scientist...each layer. The right panel illustrates a gradient tube before and after ultracentrifugation. The viral ...the bevel pointed up. A syringe at the top of the tube allows air to enter the column so the virus will...

  5. Antibody Guide

    Type
    Collection
    ...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). ...can also be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting...

  6. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...method procedure The procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant...are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60 mm dishes only 1.5 ml of each are used)....agarose plug by rapid pipetting, as shown in the YouTube video. Before returning the plate to the incubator...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun 6:7933. https... start re-conceptualizing actin filaments or microtubules. This issue is shared by other SRM techniques...

  8. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...same tube. You can clone up to 4 DNA fragments, in a specific order and orientation, in one tube, into...possible to set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    ...growing tips of microtubuli and plasmids tagged with MapTau to label entire microtubules. Find these fluorescent...best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found that too...

  11. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel, 2023. ...to protocols. In 2013, we launched the Addgene YouTube channel to add a strong visual component to our...

  12. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ... — check out Addgene’s new Quick Tip series on YouTube! In this series, we share quick tips to help you...video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols, animations, ...

  13. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...to sit down and talk with us. Tom McFadden is a YouTuber and science educator looking for creative ways...communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com.   Special thanks...

  14. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation...video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog...

  15. Deck the Lab 2022!

    Type
    Blog Post
    ...right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates...decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing routine...

  16. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...Coast where her first job was to wash Eppendorf tubes which were to be used for 3 months before they could...

  17. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer of many copies of monomeric...ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia...

  19. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...time needed to prep for an experiment (labeling tubes never takes longer than 5 minutes, right?) As a ...   Visual Planning  Plate maps, pre-labeling tubes, and even drawing out experimental steps are worth...
Showing: 21 - 40 of 174 results