We narrowed to 81 results for: wash

...requires optimization and often more stringent washing. Nonetheless, this tag can be purified with several...

...twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Prepare...twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Dilute...twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Immediately...multichannel pipette to add 200 µL wash buffer to each well. Washing can be done by hand but if available...

...plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over from the...sample and use magnets to isolate the beads for the wash and elution steps.  Both bead- and column-based ...

...stain within their membrane, while the stain is washed from the gram negative bacteria’s thinner membrane...

...immobilization on a solid support. This allows washing away of unbound material to calculate the amount...

...alongside your input control. Elute the DNA after washing and then incubate with Proteinase K to reverse ...

...purification. After binding YFP to the column, you wash several times to remove non-specific proteins, and...

...media from the 24-well plate. Wash each well with 500 µL of PBS, remove the wash, and dispose of it in an ...disposing of waste in the appropriate container. Wash 3x for 5 min in 500 µL PBS on a rocking platform...dispose of it in an appropriate waste container. Wash 3x for 5 min in 500 µL PBS on a rocking platform...

...expressing the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent...

...wells while the protein-antigen complexes will be washed away during the protocol. The amount of antibody...

...should be paired with dimmer dyes so they don’t “wash out” the rest of the panel and create spreading ...

...involves preparation of the tissue sample, several washes, and temperature changes to visualize the expression...

...white pellet of total RNA in the bottom of the tube. Wash the RNA by resuspending the pellet in 0.5–1 ml of...remove the supernatant. Remove as much of the ethanol wash as possible without disturbing the pellet. Air-dry...pellet and carefully remove any residual ethanol wash and add RNase-free water or TE as soon as the entire...

... incubator. This also means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline)...

...biotinylated target protein is added and after a few washes, binding is detected using a colorimetric reaction...

...which is particularly important for samples where washing is difficult, such as intact tissue. Some chemical...

...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...

...antigen binds to the capture antibody and a series of wash steps removes non-target proteins and molecules ...

...Then, the surgeon performs a heated chemotherapy wash throughout the abdominal cavity. This targeted chemotherapy...

...exactly when you fold them. The same with sheets – wash them and put them right back on the bed to save ...