We narrowed to 10 results for: wash

...twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Prepare...twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Dilute...twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Immediately...multichannel pipette to add 200 µL wash buffer to each well. Washing can be done by hand but if available...

...media from the 24-well plate. Wash each well with 500 µL of PBS, remove the wash, and dispose of it in an ...disposing of waste in the appropriate container. Wash 3x for 5 min in 500 µL PBS on a rocking platform...dispose of it in an appropriate waste container. Wash 3x for 5 min in 500 µL PBS on a rocking platform...

...white pellet of total RNA in the bottom of the tube. Wash the RNA by resuspending the pellet in 0.5–1 ml of...remove the supernatant. Remove as much of the ethanol wash as possible without disturbing the pellet. Air-dry...pellet and carefully remove any residual ethanol wash and add RNase-free water or TE as soon as the entire...

... column that will bind the now precipitated DNA. Wash the pellet or column with 70% ethanol to remove ...precipitation. Pour out the supernatant. Optional Wash the pellet with 70% ethanol. Note: This step removes...invert the tubes on a paper towel to drain them out. Wash pellet by adding 500 µL cold 70% ethanol. Note: ... well attached to the tube after the 70% ethanol wash. You can also pipet the supernatant out of the tube...

...deionized water 3x . Shake the gel gently between washes to remove residual water. Load the gel into the...blocking buffer for 1 h at RT on a shaking platform. Wash the membrane 3x for 5 min in 1X TBST at RT on a ... RT but may result in more non-specific binding. Wash the membrane 3x for 5 min in 1X TBST at RT on a ...antibody for 60 min at RT on a shaking platform. Wash the membrane 3x for 5 min in 1X TBST at RT on a ...

...media from the wells. Gently wash the cells with 1 mL of PBS and aspirate wash. Filter 0.1% crystal violet...violet for 10 min at RT. Gently remove the stain. Wash cells 3x with 1 mL of PBS, being careful not to ...

... shoes Gloves Eye protection or face shields Eye wash Safety shower Sharps container Biohazard waste container...splashed onto your face and/or get into your eyes. Wash your hands before and after working in the lab. ...into contact with hazardous materials. A sink, eyewash station, safety shower, fire blanket, and extinguisher...where these are located before you start. Use the eyewash station if unwanted or biohazardous materials are...

...of the recombinant antibody from the supernatant. Wash the column with 25 mL of Protein A/G binding buffer...exchange column. (Optional) Regenerate the column by washing with 25 mL of Protein A/G binding buffer and store...

...wells, using a new aspirating pipette for each well. Wash wells with 1 mL of 1X PBS and aspirate, using a ... 5 min at 100 x g . Gently aspirate supernatant. Wash cell pellets in 500 µL PBS. Centrifuge for 5 min...

...the bottom of the filter and pipette up/down and wash off the walls of the filter to recover as much virus...