We narrowed to 470 results for: Lif

1. Plasmids 101: Cre-lox

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   Blog Post

   ...uses these components as part of its natural viral life-cycle, and researchers have adapted the components...

2. Working with Nuclear Receptors

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   Blog Post

   ...website, the new version of which will feature a simplified user interface, expanded numbers of curated molecules...

3. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   .... In short, the Fragmid system takes the heavy lifting out of CRISPR vector design, making it easier to...

4. Antibodies 101: Choosing the Right Isotype

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   Blog Post

   ...If you are lucky in lab life, you will have a plethora of antibody options for your experiment (all well...

5. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   Blog Post

   ...research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1. (2018). PMID: 29326101. Additional...

6. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

   ...resonance energy transfer), and FLIM (fluorescence lifetime imaging microscopy) assays. ShadowG is darker ...

7. Fluorescent Proteins 101: Introduction to FRET

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   Blog Post

   ...pairs with improved dynamic range for fluorescence lifetime measurements." PloS one 10.8 (2015): e0134436....

8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   Blog Post

   ...fluorescent proteins for protein labeling and signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One....

9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   ...a method that uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a ...

10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ...library in question. We will order primers that can amplify the insert of the library. This PCR step also adds...

11. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post

    ...reproduction include embryonic lethality, a short lifespan, or infertility and can arise due to copy number...

12. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post

    ... resources and tools. That message was fully exemplified in Cori Bargmann’s keynote speech and in the ...

13. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post

    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

14. Tips for Titering Your Lentiviral Preps

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    Blog Post

    ...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...

15. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post

    ... skills can only assist you and make you more qualified for future career opportunities. Becoming involved...

16. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...ask your local makerspace if they want to hack new life into it! If you’re in the Victoria area, please ...

17. Hot Plasmids: Summer 2025

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    Blog Post

    ...Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s, or mGold2t. Bottom: Quantification...

18. Hot Plasmids: Winter 2025

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    Blog Post

    ... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...

19. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    ...Clinic studying host-microbe interactions in early life.    Resources More resources on the Addgene blog...

20. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post

    ...critical to the experiment, they definitely made my life easier!  In this post, I’ll share some AAV production...