We narrowed to 650 results for: CHI;

1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ...markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown on the table ...

2. Hot Plasmids: Winter 2025

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   ...inherited the genomic edit but not the editing machinery. Together, these advances have the potential to...

3. The AAVantages of AAV in CRISPR Screens

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   ...vectors can be made at high titers equal to those achieved with lentivirus but offer an improved safety profile...

4. Antibodies 101: Flow Compensation

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   ... Inputting compensation values differs between machines or the program you’re using, but usually you can...

5. Hot Plasmids and Viral Preps - May 2021

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   ...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...

6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency...

7. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ... Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S, Graham BS, Callewaert...

8. Plasmids 101: 5 factors to help you choose the right cloning method

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   ... of the post! Speed: Your grant deadline is approaching or your advisor is demanding results. If you’...

9. 10 Basic tips for mammalian cell culture

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   .... Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent...

10. Cloning Mammalian Cells with the Agarose Method

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    ...transfection efficiency is not known, this is easily achieved by splitting transfected cells into 3 plates at...

11. Anatomy of a Plasmid Page at Addgene

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    ...know the catalog number of the plasmid you are searching for, this is the quickest and easiest way to find...

12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...dengue for many years. Since more than a thousand children died of malaria today, let's not risk a potential...

13. Tips for Using BLAST to Verify Plasmids

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    ...sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know the species that...

14. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ..., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly...

15. Viral Vectors 101: The Retroviral Lifecycle

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    ...into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...

16. MXS Chaining

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    ... 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules...

17. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...have been developed to recruit transcriptional machinery to dCas9 and its RNA directed binding site. These...

18. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...

19. DNA Purification Without a Kit

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    ...method from the Bishop Lab at the University of Chicago doesn’t use actual milk to purify DNA, but rather...

20. Negative Can Be Positive: Open AAV Data with Addgene

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    ...214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,...

21. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two...

22. Lentiviral Vector Uses and Overview

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    ...Second, Tat is eliminated through the addition of a chimeric 5’ LTR containing a Tat-independent promoter. ...

23. Cpf1: A New Tool for CRISPR Genome Editing

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    ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

24. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...Addgenies from wasting hours of valuable time searching for samples and keeps us from discarding tubes...

25. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...at the center of the structure, shielded from quenching by aqueous solvent. This tightly-packed structure...

26. Hot Plasmids: Fall 2024

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    ... abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies...

27. How to Be an Excellent Trainee

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    ...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

28. Viral Vectors 101: Preparing Pooled Libraries

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    ...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...

29. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...disrupt a catalytic residue. Good news! You can achieve your precise edit without even making a break.  ...

30. Plasmids 101: Gateway Cloning

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    ... cloning plasmids here! References 1. Chee JY, Chin CF (2015) Gateway Cloning Technology: Advantages...