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...sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target...

...) antigens. Unfortunately, antibodies frequently do not work as assumed (Ayoubi et al., 2023). Additionally...may not be available to every scientist looking to do a flow cytometry experiment. But validation is critical...

...domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor...

...biology circuits. What is a molecular biologist to do? Let’s take a look at how plasmids recently deposited...JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL strains." Journal of biological...

... posts are not comprehensive, so just because you do not see a name or gene on this list, that does not...

... mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and...

...with the microscope that you will be using. If you do a head-to-head comparison right, it will be valuable...

...Central PMCID: PMC5120861. While there is much work to do, these early pilot studies have demonstrated technical...

...we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years...

...communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells, ion-based...

...interest into a bacterial cell or a host’s genome. To do this scientists commonly use phagemids, a DNA cloning...

...spatio-temporal dynamics of apoptosis in living animals. To do this they modified a different self-assembling split...

...go to grad school. But, I didn’t know what else to do. Fortunately, the head of software product management...

...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...

...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...

...broader community, and preprints are a great way to do that. We are happy to receive deposits of plasmids...

...strength of staining and background noise. If you do use different lots of your polyclonal antibody, you...

...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: ...

...sensors in terms of response and brightness. They also do not spectrally separate from green sensors and blue-light-activated.... The dosing required to achieve these efficiencies was also between 1013 - 1014 vg/kg, a dosage comparable...

...blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For...

...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...

...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...

...and Validation Group and says that she decided to do a post-doc at Novartis because she wanted first-hand...

... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

... 2025. Catalog items are updated more frequently. Do you have suggestions for other plasmids that should...

... us played with LEGO as a child (some of us still do), so we are used to the idea of general interfaces...

...RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help answer?...

...References: Monticelli, M., Ferro, T., Jaeken, J., dos Reis Ferreira, V. & Videira, P. A. Immunological ...

...invaluable resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” ...