We narrowed to 877 results for: HA

...this meeting is to _________." Everyone in the room has likely just run in from some other commitment. An...

...endonuclease or a variant thereof. This individual protein has all the components necessary to: 1. Bind to a guide...

...author plus the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

... complement the one assay per gene approach that has been the historical standard. Specifically, in our...

...as an emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays ...

...dual-vector system as opposed to a single-vector system) has several benefits. First, the low viral titers typically...

...the most widely used CRISPR nuclease, recent work has shown that alternative enzymes may better suit specific...

...experiment check out Addgene’s protocol page, which has both protocols and videos for techniques in basic...

...workshops and lessons learned: Since early 2018 the team has grown to include experts who could help provide new...

...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...

...tools). It’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one...

...protocol to work. Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated...

...particles, the functional infection unit. Each particle has a viral envelope, which fuses with the target cell...

...temporary repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn...

... the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important ...

...review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly...

...conjugate on the antibody left exposed to light?  Has this antibody been left out, gone through a freeze-thaw-freeze...

...pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman lab has a limited quantity of AAVs that can be requested ...

...since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in...

...Collin’s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and ...

...senior mentors available to go around. My advice has been to find 5 interested peers and form your own...

...light is of particular interest since blue light has limited applications in vivo. UV and blue light is...domain from A. sativa (Bubeck et al., 2018). LOV2 has a C-terminal helix that unfolds when stimulated by...

...Department of Genetics of Harvard Medical School, has a mission of raising awareness and starting conversations...

...shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium, also known as the...

...brain and muscle development. The Gronostajski lab has generated mice wherein it's possible to delete the...

...Other Resources The National Cancer Institute (NCI) has an internship similar to Susan’s NEI internship. ...

... This all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, ...

... 2008) occasionally and certainly after a set-up has been changed (for instance after replacing a broken...

...Future of Research believe that, while the mandate has been removed, institutions should still go ahead ...

...active measures in improving cross team communication has helped Addgene move from completing 2-3 large projects...

...some logical pattern across the poster. This style has the advantage of being different and potentially ...

... nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector...

...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...

...antibodies will stick permanently to a membrane that has been dried, no matter what stripping methods you ...

... exposure to increased intracellular calcium. It has been applied to mark active populations of neurons...

...for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet, you’ll...

...all of the problem solving that I did in the lab has been invaluable in my more recent positions outside...

...or capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin, both...

...afterwards. To engineer these production pathways, MoClo has been used to assemble multigene constructs capable...

...several reasons why doing something without a kit has advantages: less plastic waste, less expense, and...