We narrowed to 849 results for: HA

1. RNA Extraction Without A Kit

   Type

   Blog Post

   ...several reasons why doing something without a kit has advantages: less plastic waste, less expense, and...

2. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   ...active measures in improving cross team communication has helped Addgene move from completing 2-3 large projects...

3. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   ...Other Resources The National Cancer Institute (NCI) has an internship similar to Susan’s NEI internship. ...

4. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   ...Future of Research believe that, while the mandate has been removed, institutions should still go ahead ...

5. Celebrating 15 Years of Scientific Sharing

   Type

   Blog Post

   ...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...

6. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   ...some logical pattern across the poster. This style has the advantage of being different and potentially ...

7. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   ... nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector...

8. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   ... This all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, ...

9. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   ... 2008) occasionally and certainly after a set-up has been changed (for instance after replacing a broken...

10. Engaging with science and society at pgEd

    Type

    Blog Post

    ...Department of Genetics of Harvard Medical School, has a mission of raising awareness and starting conversations...

11. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium, also known as the...

12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...brain and muscle development. The Gronostajski lab has generated mice wherein it's possible to delete the...

13. Developing Lab Management Software for Biology

    Type

    Blog Post

    ...to the right (# this will make you sad),  someone has put in a request for a particular plasmid and now...

14. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    ...availability of both wild-type and mutant loxP sites has allowed scientists to leverage this system in new...

15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    ...can’t it’s an easy search. Writing this tutorial has shown me that I’m still in the dark ages with respect...

16. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    ...assistance in the grant application process, she has faith in you! Additionally, keep in mind that universities...

17. Plan Your Experiment

    Type

    Guide

    ...NHEJ. How do you determine that your desired edit has occurred? The exact method necessary to validate ...

18. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...opposed to an individual protein. The Bethune Lab has overcome this limitation by creating Split-BioID,...

19. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    ...know this?” Over the past fifteen years, the CBM has developed a student-centered SMART Team modeling ...

20. 6 Tips for Grant Writing

    Type

    Blog Post

    ...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ...

21. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    ...platform. During the conference If the conference has a social media policy, this should be on their website...

22. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    ...fluorescent molecules, including fluorescent proteins, has led to the alternative acronym Fluorescence Resonance...

23. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    ... Recently, a fourth generation packaging system has become available through Clontech. This system improves...

24. Pooled Library Amplifications

    Type

    Blog Post

    ...replicate faster. For this reason, if your library has inserts of different sizes (such as a transcription...

25. Antibodies 101: Flow Compensation

    Type

    Blog Post

    .... This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells and cancer ...

26. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    ...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...

27. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    ... Prize in Chemistry for his work. Since then PCR has become a standard and essential practice in molecular...

28. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    ...particularly after the majority of on-target editing has occurred. Anti-CRISPR proteins can be used to limit...

29. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    ..., another method to create point mutations, also has a strict editing window about 15 nt upstream of the...

30. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    ...particular poster you are interested in viewing which has drawn a large crowd, making it difficult to see the...

31. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    ...promises and associated sequence information. Addgene has partnered with the BioBricks Foundation to distribute...

32. Design Tips for Prime Editing

    Type

    Blog Post

    ...prevents the prime editor from re-engaging with DNA it has already edited. Created with BioRender.com. ...

33. Great Results Start with Great Standard Curves

    Type

    Blog Post

    ...sample, called a standard. The ideal standard curve has at least five dilutions, with each step of the series...

34. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    ...While the method is simple and saves time, it also has several drawbacks. Direct ELISAs generally have higher...

35. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    ...titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing titering...

36. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    ...transcription template (RTT). The design of the pegRNA has been shown to play a critical role in the editing...

37. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    ...since bacteria won’t divide immediately after it has been heat-shocked or electrically zapped. As a result...

38. How to Be an Excellent Trainee

    Type

    Blog Post

    ...the bench waiting for you, prep for research work has to be done by the researchers themselves.  Be ...

39. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    ...Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious...

40. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    ... from R1 institutions to community colleges, and has helped provide original research experiences for ...