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  1. Using Addgene's New Viral Service for Your Research

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    Blog Post
    ...for similarly controlling neuronal activity with light will be available soon. We’re also distributing ...controls to determine if your AAVs are getting to the right cells using your particular delivery technique and...

  8. 5 Great Apps for Lab Life

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    Blog Post
    ...videos. The search function in the manual just highlights what the user searches, which is not very helpful...

  9. Improving Transduction Efficiency with Exosome AAVs

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    Blog Post
    ...). They saw that these AAV particles come in a slightly different shape. These AAV particles are often...purified AAV vectors. They found that they have a higher transduction efficiency, i.e. the same number of...

  13. Networking Follow-up: I Met a Person. Now What?

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    Blog Post
    ...out of the incubator because I’d had a baby that night, all he said was “OK” and hung up on me). I went... 25 years since our last contact, but we were delighted to be reconnected.  Science can be a small community...

  14. 25 Work From Home Tips for Scientists

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    Blog Post
    ...great way to take a mental break, while meditating right before bed will help you prepare for sleep. At Addgene... Home. It’s normal and okay to feel overwhelmed right now, but hopefully these tips are helpful to you...

  15. Pushing the Limits of DNA Assembly

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    Blog Post
    ...makes NEBuilder HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset...covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc...

  17. Plasmids 101: Gram Negative and Positive Bacteria

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    Blog Post
    ...dry, and then view under an oil immersion using a light microscope. Gram positive bacteria will be stained...decolorization. In contrast, counterstain, safranin, is a light and water soluble dye that is able to enter the ...

  18. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...can't be too hard to apply. These papers suggest it might work well enough to consider as a therapeutic.” ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence...

  19. How to Lead a Great Meeting

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    Blog Post
    ...next steps (see “action items” below). Get the right people in the room There are few experiences more...informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When no discussion...

  20. Management for Scientists: Delegating is Key

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    Blog Post
    ...bandwidth to become an expert) the end result is high quality and the entire process is completed more... What types of issues do you want to know about right away? Provide adequate support. The manager must...

  21. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ...however, they are different viruses and thus use slightly different isoforms of these packaging components...research to reduce promoter interference and/or drive high expression of a transgene from an internal promoter...

  22. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...and their functions are more intuitive than you might think. So, if you’ve ever wondered what goes into...polymerase II promoter and terminator regions and are slightly different in sequence. Both LTRs do share common...

  23. Viral Vectors 101: Biosensors

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    Blog Post
    ...proteins of interest such as actinin. When tension is high vs. low in the cell the donor and acceptor of a ...on changes to the structure of actinin in low vs. high tension. These systems are powerful tools to measure...

  24. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity... Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression ...

  25. Targeted Mutagenesis with EvolvR

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    Blog Post
    ...middle) and extends from the 3' end of the nick (right), occasionally introducing mutations (red nucleotide...EvolvR with a targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard...

  27. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ... and Andrew Hempstead. What do smell, taste and sight have in common, besides being one of the five senses...Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors are activated...

  31. Addgene: The First Twenty Years

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    Blog Post
    ...viral vectors were useful for researchers wanting high quality reagents, and we realized we could use the...Addgene’s support, could fill a community need for high-quality service, open data, and increased reproducibility...

  32. Viral Vectors 101: An Introduction to AAV

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    Blog Post
    ....7 kb. Co-infection rates for AAVs are generally high enough for this approach to work, although it’s ...time. Though the time delay until expression is highly dependent on the serotype and target tissue, two...

  33. Summer SciComm: Preprints

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    Blog Post
    ... often post a preprint at the same time as, or slightly before, submitting the manuscript to a journal...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

  34. Visualizing Protein Turnover In Situ

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    Blog Post
    ...amino acids. The use of radioactive materials is highly regulated, requires designated work areas and come...passionate about communicating science to all audiences. Right now, she is a science instructor at The Innovation...

  35. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ...microenvironments and what encircles a disease is highly prone to affect it. While tumors may be the stars...surroundings and being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated...

  36. dTAG - You're it!

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    Blog Post
    ...mouse models. The dTAG system The dTAG system pairs highly selective small molecule FKBP12F36V degraders (...observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature...

  37. Plasmids 101: Modular Cloning

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    Blog Post
    ...of large-scale assembly useful, you may ask? You might be surprised by how multi-purpose it can be. Let... Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with NEB’s DAD Interview...

  38. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    Blog Post
    ...are a multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms ...AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70% among the human population. These pre-existing...
Showing: 761 - 800 of 978 results