We narrowed to 919 results for: IRE;

1. From our Table to Yours: An Inside Look at Lunch at Addgene

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   Blog Post

   ...divided into smaller functional teams. Our Executive Director, Joanne Kamens, responded by starting something...

2. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

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   Blog Post

   ...the time that would be spent making and testing entirely new gRNA designs. You can now easily find many...

3. Six Spooky Science Stories and Halloween at Addgene

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   ...for the next unsuspecting ant to stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory...

4. A new platform for fast functional characterization of potentially cancerous mutations

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   ...cell viability, and these “activators” may be the direct drivers of tumor formation. Being able to parse...

5. Easing the Protein Purification Process with pCri

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   ...stressful lab activities. Working with proteins requires a substantial amount of properly folded, relatively...

6. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

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   ... own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you realistic expectations...

7. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

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   ...Publication Citing this Plasmid” - this simple act directly associates you with a network of researchers who...

8. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

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   ...AAV into the brain can usually be achieved by a directed brain injection, but some serotypes can cross ...

9. What's New in CRISPR - September 2019

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   ...gene) on the donor vector. Therefore, targeting requires two sgRNAS: one that targets the knock-in site...

10. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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    ...constructs to support SARS-CoV-2 research.  Browse the entire Ginkgo Bioworks COVID-19 plasmid collection ...

11. Scientific Sharing in the Time of COVID-19: Databases and Resources

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    Blog Post

    ...virologists who don't have the resources to get directly involved with COVID-19 to researchers in other...

12. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post

    ...for the scientific community. As the executive director of Addgene, it is my privilege to uphold the values...

13. Cre-ating New Methods for Site-specific Recombination in Drosophila

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    Blog Post

    ...toolkit for specific recombination patterns that require multiple recombinases. These tools should further...

14. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post

    ...community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael Davidson...

15. 9 Tips to Achieve Success in Academia

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    ... – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like or artistic; ...

16. In Living Color: The Skinny on In Vivo Imaging Tools

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    ...nm range, and his team is also looking in the direction of biosensors capable of revealing, for example...

17. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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    Blog Post

    ...experiment, not the paper. To get around reading through entire papers, perhaps you search google images for specific...

18. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    ...flexible. The best stuff that I do is not what I was hired to do.” Get Networking Tips Here And when asked...

19. Some Like it Hot: Thermostable GeoCas9

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    ... with in vitro molecular bio applications that require high temperature reaction conditions. Finally, ...

20. SunTag and Fluorescent Imaging

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    ...How does SunTag fix these problems? Instead of directly fusing a fluorescent protein to your protein of...

21. Bricking Science: Portraying Scientific Reality Through LEGO

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    Blog Post

    ...consuming; producing a 1-2 minute video takes an entire week of our free time. After taking almost a thousand...

22. Tips for Improving Your Next Manuscript

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    ...with a facilitator. In the focus groups, we were required to critique two of our colleagues’ manuscripts...

23. Addgene at the ASM Microbe 2016 Conference

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    ...sphaeroides!) at the ASM booth. As well as being the Director of the Great Lakes Bioenergy Research Center and...

24. Networking and Inspiring Young Scientists at Freshers Fairs

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    ...Chemical Society in University College Dublin, Ireland The Chemical Society was founded in 1944 with the...

25. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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    ... amino acid, the bacteria will only grow if it acquired the plasmid containing the gene necessary to synthesize...

26. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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    Blog Post

    ...undergraduate student at Northeastern University, I am required to complete a six month work experience called...

27. Antibodies 101: Fc Effector Functions

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    ...cellular cytotoxicity (ADCC) is a slightly more direct method than opsonization. In ADCC, IgG antibodies...

28. Cellosaurus: A Cell Line Information Database

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    ... used in some cases, but much of this work has required manually going through the pdfs of the 26,000 ...

29. We're Updating Our Hold for Publication Policy

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    ... internally. And remember, a publication isn’t required to share your plasmids! You can make your deposit...

30. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

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    ... more variety and more power to influence the direction of my work. My master’s gave me a deeper foundation...

31. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    ...Calculators Primer resuspension calculator (sign-in required.)  DailyCalcs Science Calculator app for iPhone...

32. 10 Ways to Share Your Science!

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    ...research, SciFund is really motivated more by the desire to help close the widening gap between science ...

33. Plasmids 101: Antibiotic Resistance Genes

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    ...Antibiotics can be categorized based on whether they directly kill bacteria (bactericidal) or slow growth/prevent...

34. Choosing a Good Mentor for Scientists

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    ...PI will be a good role model and teacher. Do you admire her management style? Does he have ethical behavior...

35. Science Careers: Unruly Interests Feed Many Paths

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    ...complex - and unique - adult. My initial research directions followed that avenue with projects studying developmentally...

36. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post

    ...times which limits its use for imaging a protein’s entire life-cycle. This makes it nearly impossible for...

37. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...demonstrate that these results were, in fact, due to impaired endocytosis. In parallel assays designed to test...

38. Summer SciComm: Science Storytelling

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    Blog Post

    ...opposing forces or (2) the result of competing desires or (3) the presence of obstacles that need to be...

39. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post

    ...order ready-to-use antibodies for your experiments directly from Addgene. How Addgene is Reimagining Antibody...

40. Hot Plasmids and Viral Preps - September 2021

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    ... from 36 μg/mL to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest...