We narrowed to 801 results for: MAK

1. Analyzing CRISPR Editing Results with ICE from Synthego

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   ...engineering has revolutionized the gene editing field by making experimental workflows considerably easier, faster...

2. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...through smart engineering Different FLP versions – making FLP efficient The first version of FLP discovered...

3. Plasmids 101: Plasmid Incompatibility

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   ...t simply rely on chance (or random diffusion) to make this happen.  There are several different versions...

4. CRISPR-mediated Plant Base Editors

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   ... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

5. Antibodies 101: Immunoprecipitation

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   ... and high specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must...

6. Viral Vectors 101: Pseudotyping

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   ...retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity....

7. To Codon Optimize or Not: That is the Question

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   ...organism's codon bias. The idea is that scientists can make mutations throughout a gene of interest based on...

8. Bioinformatics at Addgene

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   ...bbdone with our bbduk process.    At first, to make sure we understood the concept of channels and implemented...

9. Transferable Skills Guide: Creativity

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   ...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...

10. CRISPR Activation: A Practical Guide

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    ... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger...

11. Tips for CRISPR Gene Editing in Mice

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    ...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

12. The Challenges of Cell Culture

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    ... generally ensure cell line identity and purity making it easier for comparable studies to be performed...

13. Getting the Most from Your Lentiviral Transduction

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    ...physiology of different cell types, however, can make this assumption incorrect. One of the earliest steps...

14. Plasmids 101: Control Plasmids

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    ...reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)" and should...

15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...allowing for stable expression of the transgene. This makes IDLVs an attractive choice for certain types of ...

16. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...controls So how can you address these errors without making incorrect assumptions? The answer depends on the...

17. Antibodies 101: Beyond Surface Labeling

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    ...gating uses the same color for different markers, making it easy to gate on the negative cell population...

18. CRISPR 101: Homology Directed Repair

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    .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

19. Hot Biosensors 2022: Year-End Roundup

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    ...iGECI and NIR-GECO2G, and its small size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano...

20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...position. The “sloppy” PCR reaction is created by making some or all of the following modifications: 1) ...

21. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch...

22. Plasmids 101: Biotinylation

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    ...exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding...

23. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging capacity...

24. Evolution of Lab Techniques

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    ...popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic mouse lines...

25. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...model than in latent HIV-1 infection, which would make it easier for an “escape” virus to propagate and...

26. A Control for All Seasons

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    ... high degree of variability in the process could make it difficult to obtain meaningful results.  Biological...

27. Plasmids 101: Repressible Promoters

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    ...adds a repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...

28. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...register at a time. If a particular application makes it difficult to isolate single registers or if it...

29. Plasmids 101: Restriction Cloning

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    ...overhangs that allow them to bind. Ligase is used to make bonds between the insert and backbone covalent.  ...

30. Finding Your Perfect Job After University

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    ...Addgene’s Website and travel to meet scientists, making my job different every day. I love the freedom ...

31. Deep Dive: Statistical Tests (Comparisons)

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    ... friendly neighborhood biostatistician happy by making your statistical analysis plan when you design ...

32. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...studies, the high gene copy number found in plants makes traditional knockouts unattractive from a practical...

33. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    ...selective expression of opsin genes in targeted cells makes it possible to control neuronal activity with a ...

34. Sequencing Options for CRISPR Genotyping

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    ... and do not require high sequencing read counts making them fast and viable options for multiplex sequencing...

35. Cancer and the Immune System: Deciphering the Relationship

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    ...death receptor presentation on tumor cells thus making these cells more susceptible to elimination by ...

36. Antibodies 101: Flow Cytometry

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    ...cytometry can help you overcome these challenges and make these types of experiments easier and faster to ...

37. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    ...development. These are required for a growing organism to make the complicated transition from a clump of cells...

38. A Primer on Optogenetics: Introduction and Opsin Delivery

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    ... don’t want to spend months doing surgeries and making fiber optic cables only to hook up your lasers ...

39. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...18754011. 43. Zhou, Q. and D.A. Melton, Extreme makeover: converting one cell into another. Cell Stem Cell...

40. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    ..., 2020.   Scientists around the world have been making major improvements to CRISPR technology since its...