We narrowed to 809 results for: MAK

...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

...through smart engineering Different FLP versions – making FLP efficient The first version of FLP discovered...

...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...

...engineering has revolutionized the gene editing field by making experimental workflows considerably easier, faster...

... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

...organism's codon bias. The idea is that scientists can make mutations throughout a gene of interest based on...

...t simply rely on chance (or random diffusion) to make this happen.  There are several different versions...

...bbdone with our bbduk process.    At first, to make sure we understood the concept of channels and implemented...

...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...

... and high specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must...

...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

...often looks like the graph on the left, it might make more sense once you realize events are plotted on...

...retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity....

... long latency period, and rarity of mesothelioma make it different from many other types of cancer. Mesothelioma...

...until 5 pm). The ability to see the questions first makes Reddit a great platform for those who aren’t so ...

... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger...

...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

... generally ensure cell line identity and purity making it easier for comparable studies to be performed...

...physiology of different cell types, however, can make this assumption incorrect. One of the earliest steps...

...reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)" and should...

...position. The “sloppy” PCR reaction is created by making some or all of the following modifications: 1) ...

...exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding...

...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging capacity...

...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch...

...iGECI and NIR-GECO2G, and its small size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano...

...allowing for stable expression of the transgene. This makes IDLVs an attractive choice for certain types of ...

...controls So how can you address these errors without making incorrect assumptions? The answer depends on the...

...gating uses the same color for different markers, making it easy to gate on the negative cell population...

.... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

...popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic mouse lines...

...model than in latent HIV-1 infection, which would make it easier for an “escape” virus to propagate and...

...adds a repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...

...Ahituv Lab and collaborators have found a way to make adipocytes fight cancer. In this method, called ...

... high degree of variability in the process could make it difficult to obtain meaningful results.  Biological...

...register at a time. If a particular application makes it difficult to isolate single registers or if it...

...overhangs that allow them to bind. Ligase is used to make bonds between the insert and backbone covalent.  ...

...Addgene’s Website and travel to meet scientists, making my job different every day. I love the freedom ...

...selective expression of opsin genes in targeted cells makes it possible to control neuronal activity with a ...

...studies, the high gene copy number found in plants makes traditional knockouts unattractive from a practical...

...-antibody interactions in a plate-based format, making it suitable for comparing relative affinities across...