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We narrowed to 1,006 results for: RING

Showing: 761 - 800 of 1006 results

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...Neighborhood.   Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance ... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    ...about. Instead of reading a paper and getting my bearings, I would read a paper, panic, and then download...think the field is going or what areas are worth exploring.  Edit and rewrite, then repeat If you’re like...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...biology because of an image that was honestly quite boring. My Bio 101 professor displayed a codon table —...mammalian cells like neurons, may not replicate during the course of your experiment, meaning you don’...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...

  12. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

  14. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...mesothelioma. Asbestos, and certain other naturally occurring asbestiform minerals such as erionite, are the...an immediate stop to the use of asbestos, thus ensuring that future generations will be protected from...

  16. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...high protein levels including:  ompT: Strains harboring this mutation are deficient in outer membrane ...recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression...

  18. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...sequence detection method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127... Detection of SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159...

  19. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...vivo probes, due to low autofluorescence, low scattering, and deep penetration of red-shifted light. The...about cellular events via  fluorescence changes occurring due to protein synthesis, degradation, or reconstitution...

  20. March for Science

    Type
    Blog Post
    ...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold... “politicized science” ABC News: White House considering gutting 38 EPA programs: draft budget  Science...

  21. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...'s about how YOU will fit into a department and bring in grants. What kind of colleague will you be? Do...

  23. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...Concordet J-P. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection...

  25. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...seems to be the case that there is no universal scoring system for selecting a gRNA, as the method of producing... is taken in the interpretation of results by requiring multiple sequences targeting a gene to score in...

  27. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional Organization Resources on...

  28. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...looking for, but there are also benefits gained by lowering the complexity of the screen. For example, the..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  29. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ... most common method is transfection of short interfering RNA (siRNA). Knockdown caused by siRNA is also...cytometry experiment. But validation is critical to ensuring accurate interpretation of your results. Therefore...

  30. CRISPR Guide

    Type
    Guide
    ...scientists have modified for a wide range of genome engineering techniques . Collectively, these techniques are...R epeat) technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) ...CRISPR rapidly became the most popular genome engineering approach. Figure 1: Overview of the basic CRISPR...first and come back to HDR . NHEJ is a naturally occurring repair pathway and is the primary repair mechanism... for DSBs. While this pathway is efficient in repairing DSB, it frequently causes small nucleotide insertions...: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve editing more ...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs...

  31. 2022 Depositor Week

    Type
    Blog Post
    ...revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

  35. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources...

  36. We’re Going Viral This Week!

    Type
    Blog Post
    ... post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also publishing research...

  40. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge...
Showing: 761 - 800 of 1006 results