We narrowed to 903 results for: nes

...widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively simple and robust...

...comparison of the same experiment from different cell lines/model systems ReFigure allows you to make meaningful...

...neurons using the Gal4/UAS system. This created fish lines with different groups of neurons that could be activated...

...vectors (shapes defined by 2D points connected by lines and curves) that you’ve drawn. Layers are useful...

...Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background with...

...were invented by synthetic biologists. An sgRNA combines a crRNA and a tracrRNA into a single strand that...

...Camelids. The declaration is intended to raise awareness of the economic and cultural importance of these...

... the road to keep being at the very top of the business.” Caitlin’s advice for budding science communicators...

...bacteria and plants. Her research involves cloning cry genes from the soil bacterium Bacillus thuringiensis and...

...where we are often trained to follow protocols, guidelines, and standards. Bricking science reminded us ...

...Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression...

... support for a range of different scientific disciplines, as well as social and networking events. Imperial...

...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

...science really works. 2)   Read science writing in magazines or newspapers or other places online, and consider...

...condition known to the plant community as ‘plant blindness’ - the tendency for students to be more interested...

...affinity for the ligand, especially if the modified amines are located near the ligand binding site. A selective...

...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...

..., we hope we can help all researchers achieve happiness in their careers regardless of their end goals...

...the spectrum (above 600 nm) and increasing the brightness. Arne Fabritius and colleagues started with the...

...enhance the therapeutic potential of antibody medicines. In this process, antibodies derived from non-...

...achieving knowledge. Or, as we in the storytelling business like to say, science is all about navigating conflict...

...plasmid is expected to be >12 kb. However if it recombines at the 5’ and 3’ LTR regions, it splits into ...

...plasmid which contains the structural and packaging genes, the adenoviral helper plasmid which contains the...

...deposits. We were excited to deliver the very first ones in person by our own Tech Transfer Team! Why MTAs...

...including his zinc sensor and his genetically encoded magnesium sensor, the first of its kind. “I hope by depositing...

...track progress, adjust expectations, and record milestones in reaching goals. The more time and thought ...

...with a large range of characteristics (color, brightness, photostability, maturation, oligomerization)...

...lot of AAV particles are needed to get a few good ones into a cell to produce expression. The higher the...

...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...

...cells in the inner ear in a mouse model of human deafness (György et al., 2017), exo-AAV2 in mouse retina...

...read and follow the journal’s specific author guidelines for reporting your materials. If you deposit ...

...as informed consent (How do we prevent stem cell lines from becoming the new HeLa cells?), genome engineering...

...Agreement (UBMTA) is one of the most commonly used ones for transfers between academic institutions.  What...

...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

... lower its aggregation tendency, increase its brightness, and decrease the amount of light required for...

...of non-plasmid DNA or heterogeneous and/or mixed clones present in the original sample. At seqWell, we ...

...heard. Here's a template for team meetings. One-on-Ones: Managers should set up regular check-ins with their...

...complicated (and again, expensive) the different machines are, and how many regulations are in place to ...

...many of them focused more on social media or the phones hiding under their desks than listening to me. ...

...-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic...