We narrowed to 988 results for: IGH@

...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

...actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about...

...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

...education. If you suspect that science communication might be for you, don’t wait. Start communicating now....

...for a lay readership, and working with middle and high school students. One of these organizations was ...

...species one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia ...

...western blot as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it ...

...your desired genes. Remember Punnett squares from high school biology? They will come in handy here! Crossing...

...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,...

... job appealed to us. However, since the lab was highly focused on protecting intellectual property, it...

...Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated...

...requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever...

...some classic protocols on Protocol Online that you might find useful for your research. LabGuru Website: ...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...platform. Pour off the water in the sink. Take a brightfield image of the gel with an appropriate imaging ...25 kDa corresponding to the heavy chain (HC) and light chain (LC) proteins, respectively. There should ...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...through a 0.2 µm PES filter. Aliquot and freeze upright at -20 °C Procedure Section 1: Affinity chromatography...and gently pour off the ethanol storage buffer. Tightly attach LabMate PD10 Buffer Reservoirs to the top...

...constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target...

...vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared...

...with one or more of these parameters can lead to a high number of false negatives in Y2H screens. Testing...

...bacteria deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number...

...amplification in the host cell. This enables relatively high and long-term expression of the reprogramming factors...

...hosts. Also, self-transmissible plasmids may have high copy numbers and reduced metabolic load (Meyer 2009...

...externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists can make...

...transduce multiple tissues. So how do you choose the right serotype? Keep asking questions!  Table 1: AAV Serotypes...

...bases. This is an example of a trace file from a high-quality portion of a sequencing reaction: Sequence...file from a portion of a sequencing reaction with high background: If you obtain a different sequence than...

...Distilled water Disinfectant Thermometer Water bath weights and floats Reagents None needed Procedure Ensure...be used to secure tubes in place and water bath weights can hold bottles in place. After putting your tubes...

...your plasmid and order primers (see figure to the right). When designing your plasmid, think about what ...fragments can subsequently anneal to each other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides...

...extinguisher Guidelines BSL-1 Guidelines Before You Work Right after entering the lab, put on the proper personal.... BSL-2 centrifugation steps require an aerosol-tight lid and rotors should be loaded and unloaded in ...

...reaction, incubation time can range from 45 mins to overnight. For diagnostic digests, 1-2 hours is often sufficient...and can happen for a variety of reasons, including high glycerol concentration. Learn more at (Link opens...

...Incubate at room temperature for 2hr, or at 16°C overnight (following the manufacturer’s instructions). Note...For many ligation reactions, especially if using "high concentration" ligase, 5min at room temperature ...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ... 0.2 µm PES syringe filter. Aliquot and freeze upright at -20 °C. Procedure Section 1: Seeding cells The...

...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....appropriate PCR tubes. Pipette back and forth 5 times. Lightly cap the PCR tubes. Generate the Droplets Bring ...

...C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 minutes at 10,000 x g at 4°...

...well enough to be snug to your hand, but not so tight that you cannot perform your duties. Be sure to ...

...#3. Incubate plate with newly plated bacteria overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids ...

... 6 cm diameter LB/agar plates without antibiotic High concentration (100 mg/mL, 1000x) carbenicillin stock...

...Addgene Depositing Page Career Videos Get career insight and advice from real Addgenies! (Link opens in ...

...also be used for PCR-mediated DNA mutagenesis. A higher cation concentration increases the error rate of...