...continue to accelerate research and discovery by improving access to useful research materials and information...have been lost to continue to be available to the research community.    Validation For most cloning projects...construct by Sanger sequence analysis. But for this research, it was likely that the sequence of the functional...range secondary antibodies are commonly used in research laboratories to detect the different subclasses...

...iPS cells and embryos make a case for conducting research in embryos should our society decide that germline...not being used to establish a pregnancy, the researchers were able to examine each individual blastomere...embryos and iPS models, it appears that embryo research is the only way to answer questions of efficiency...Given moral and ethical concerns surrounding such research, it is crucial that scientists proceed with caution...non-biologists alike. Given the public significance of this research, we have the obligation to discuss it as openly...

...discovery, researchers have done significant work to improve the Adv genome’s utility in research and its...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced...PMID: 19373227. 4. Mittereder, Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation of the concentration...Resources on the Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of...

...experimental tools. They certainly are very handy in research, but we’re really borrowing these tools from Mother...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA...plasmids can be dangerous, they also provide researchers with new means to deliver DNA and other molecules...tend to be small and poorly studied, but some researchers have suggested they may have relevance to human...natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid tools...

...team effort and many late nights, not unlike most research, but the urgent need for the test did catalyze...yet never hesitated to reach out to the greater research community when we needed additional expertise....rapid sharing of these tools with the Zika virus research community. Find the Zika Sensor Constructs at ... of Pharmacy at the University of Toronto. His research is focused on developing cell-free synthetic biology...Arizona State University. His group carries out research in synthetic biology, nanomaterials science, and...

... technical challenges they can present. Many researchers find themselves plagued by troubleshooting a ...sensitive to freeze thaw cycles. Depending on the researcher and the type of virus, there have been varied...virus will be used in a couple of days, some researchers prefer to store freshly harvested virus at 4 ...transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603. PubMed PMID: 12036457. 6...transfected DNA into normal human cells."Nucleic acids research 18.9 (1990): 2733-2738. PubMed PMID: 2339059. ...

...continue our mission of accelerating scientific research. To make sure that adeno-associated viral (AAV...impurities are generally considered innocuous for research-grade vectors, we’d like to make sure they are...reference sequence. Second, we perform a megaBLAST search on all the reads that did not map to the reference...clinical-grade materials, and is not commonly performed for research-grade materials. Addgene is going the extra mile...that you receive the best AAV vectors for your research. Please use the comments section below to let ...

...molecular biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression..., biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them... also be purified for use in molecular biology research (eg. polymerases and other enzymes might be purified...and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control...Resources on Addgene.org Bacterial expression systems Search for plasmids by expression system ...

...fertilization treatments and donated afterwards for ESC research purposes, with the informed consent of the donor...and adult stem cells can be used for different research and therapeutic purposes. Both types have their...skirts issues surrounding the use of embryos in research. With all these benefits, iPSCs have increasingly...increasingly become the ideal experimental system for research in regenerative medicine over the last decade....Richards, History and perspective of stem cell research. Best Pract Res Clin Obstet Gynaecol, 2004. 18...

...biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots By Emily...New recombinant antibodies for neurodegeneration research   By Ashley Waldron We recently added two antibodies...against important targets for neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both ...hope these will become valuable tools for your research! If they do (or if they don't!), please consider...consider sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  ...

...a strategy suited to their research. Find Plasmids for Stem Cell Research Delivery Method Mechanism...layers. Choosing a reprogramming method Most iPSC research falls into two categories: 1) studies focused ...at least one method that can help you with your research goals. Are you a new or experienced reprogrammer...The promise of induced pluripotent stem cells in research and therapy." Nature 481, no. 7381 (2012): 295... Cells Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate...

...acknowledge that negative data is something every researcher experiences and that it often provides incredibly...and the CLOVER Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists...regions and cell types. Power in numbers A Pubmed search for “AAV” and “brain” over the past decade gives...., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives on Data Sharing in Deep Brain Stimulation...Data Freedom: The Expansion of Data Sharing in Research Publications...

...types in the sample. The visualization helps the researcher see which of the multiplexed sgRNA was involved...browser’s Control+F or Command+F functionality to search for a particular sequence or name. You can perform...educating and supporting novice to expert-level researchers in achieving consistently successful CRISPR experiments...enthusiast, and when she is not guiding CRISPR researchers, she's either day-dreaming about exploring sci-fi...our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources ...

...selection. In a subcutaneous cancer model, the researchers showed that after 24 hours, the unstabilized ...in nature, these systems have been adapted by researchers for many uses when plasmid retention without ...for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies using...genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369. Kulakauskas....org Check out our molecular biology reference Search for a plasmid here Find plasmids from our special...

...guest bloggers Marcelle Tuttle and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa for specific gene targets CRISPR activation...scale of activation allows for exploratory-based research as well. For example, CRISPRa can be used to find...medical student at Tufts University and former researcher at the Wyss Institute for Biologically Inspired...Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and transcription...

... in Biomedical Research from Albert Einstein College of Medicine. Her doctoral research was conducted ...Figure 1). When behaving properly, FPs enable researchers to investigate the localization and dynamics ...co-mentorship Blossom Damania and Jack Griffith, her research focuses on the replication machinery of Kaposi...secretory pathway."Biochemical and biophysical research communications 430.3 (2013): 1114-1119. Pubmed...

...exist for a variety of species, cell types, and research applications. Labeling neurons with Brainbow The... used alongside common, existing tools for fly research that use Flp recombination. In addition to the...small population size of CFP++ cells permitted researchers to longitudinally map the turnover of Langerhans...such as the brain, transparent so that the 3D architecture of a fluorescent neuron is visible. Chemical...more details, check out this blog post from the researchers who developed Tetbow. Conclusion Stochastic multicolor...

...expression or silencing. To alter DNA modifications, researchers used blunt tools like histone deacetylases, but...epigenetic modifications at a user-specified locus. Researchers showed that TALE-TET1 constructs, which fused...transcription at CpG regions of various promoters. Other researchers additionally fused a TAL effector to LSD1 histone...and dCas9-based epigenome editing. Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286...system for targeted DNA methylation. Nucleic Acids Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159...

... selection marker such as thymidine kinase, a researcher may subsequently kill off any cells that still...later restores the target sequence. This allows researchers to avoid making unnecessary base pair changes...powerful technology that may be used for a variety of research applications, including making transgenic cell...molecular and cellular biology and brings 10 years of research experience with stem cells to Transposagen's cell...Mathew applies his specialized knowledge to help researchers identify the right technologies and strategies...

...on the right-hand side of the search page to narrow your keyword search to one of these organisms. Help...plasmid kits and other resources for microbial research. Kits, guides, plasmids, and more We have Plasmid...resources that we created filters just for them on our search page. Use the Expression and Insert Species filters...