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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 403 results for: CAPS

Showing: 381 - 400 of 403 results

  3. Finding Your Science Policy Path

    Type
    Blog Post
    ...well-endowed philanthropy who can shift the funding landscape to focus on a neglected disease area. Depending...

  10. Protocol - How to Design Primers

    Type
    Protocol
    ... of a regular nucleotide for broader pairing capabilities. Taking into consideration the information above...

  11. Water Bath Protocol

    Type
    Protocol
    ...bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside the water bath...

  14. Pipetting Protocol

    Type
    Protocol
    ... of and gently in contact with the container (capillary action between the container and the liquid will...

  15. Kit Free RNA Extraction

    Type
    Protocol
    ...vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar product such as TRI Reagent...

  16. Antibody Guide

    Type
    Guide
    ...antibodies are capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...also be included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP...variation of IP uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and...methods. Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate...Amplification Application Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods...

  17. CRISPR Library Amplification

    Type
    Protocol
    ...overloaded. If you find that your pellet outstrips the capacity listed in the Maxiprep protocol, scale the reagent...

  18. Adenovirus Guide

    Type
    Guide
    ...viral particle and are only composed of a protein capsid and the viral genetic material inside. Adenoviral...structural proteins needed to assemble icosahedral capsids and build new virions. Figure 1: Wild-type adenovirus...physical space available inside of the adenoviral capsid and directly correlates to the size of the DNA ...vectors and further increase the transgene packaging capacity up to ~10.5 kb. The deleted E2 and E4 regions ...vectors are also referred to as "gutless" or high capacity AdV (HCAdV) vectors. Here, all viral sequences...of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector. pAdEasy-2 does not contain...producing viral vectors in combination with foreign capsid proteins. Researchers have further refined the ...

  19. CRISPR Guide

    Type
    Guide
    ... others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise DNA-targeting...programmability for different genomic loci, and the capability to detect multiple targets simultaneously. CRISPR...and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian Xu lab , utilizes... Cas12a also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single...carrying an ADAR truncation that retain RNA editing capabilities but are small enough to be packaged in AAV particles...two nuclease domains inactivated. This enzyme is capable of cleaving only one strand of target dsDNA. Off-target...
Showing: 381 - 400 of 403 results