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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 650 results for: CHI;

Showing: 381 - 400 of 650 results

  3. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....experience.  Can combines cons of multiple medias. Switching from one media type to another is cognitively ...

  5. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...limited in the level of resolution that they can achieve. This means that regardless of how powerful of ...evenly in all directions, aka isotropically. But achieving isotropic expansion depends on how well the gel...

  6. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...opportunities for his students. But he also found that his teaching responsibilities left him with limited time for...the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent training...

  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA...publicly available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins...

  8. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...when interpreting findings, particularly when researching ancestry groups. And, of course, it’s imperative...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...

  12. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... yourself, “I’m not married,” or “I don’t have children, so my life will be under control.” Think again...starve while you are away a few days. Also, your children will enjoy day care and school if you tell them...

  13. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...hope that a similar reversal may be possible for children and adults with Rett Syndrome.  Since these discoveries...these approaches, such as delivery of the editing machinery to the brain, minimizing off-target editing, and...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    ...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review...Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your...

  16. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...plants." Plant Biology 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed...

  18. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...efficient base rewriting in the genome became achievable in 2016 when two independent groups (Komor et...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun ...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366...rate of full‐length human proteins expressed in Escherichia coli." Protein Science 19.7 (2010): 1312-1326...
Showing: 381 - 400 of 650 results